Humanized anti-tag-72 monoclonal antibodies using human subgroup 4 kappa light chains

ABSTRACT

Novel composite and humanized anti-TAG-72 monoclonal antibodies, antibody fragments, and derivatives thereof using human subgroup IV kappa light chain framework regions.

CROSS-REFERENCE TO RELATED APPLICATIONS

The Applicants herein claim the benefit of priority under 35 U.S.C. § 119(e) to U.S. Provisional Application No. 60/030,173 entitled, “Humanized Monoclonal Antibodies Specific to TAG-72, Methods for Their Manufacture and Usage in the Treatment or Diagnosis of Cancer,” which was filed on Oct. 31, 1996 by W. H. Kerr Anderson et al., and which is now abandoned. The present application is a Continuation-in-Part of application Ser. No. 08/261,354, filed Jun. 16, 1994 now U.S. Pat. No. 5,976,531, which is a Continuation-in-Part of application Ser. Nos. 07/510,697, filed Jul. 17, 1990, and 07/964,536, filed Oct. 20, 1992, both now abandoned.

GOVERNMENT INTEREST

The invention of HuCC49*, as described in Example 6, was made in collaboration with the United States government under CRADA CACR-0014 granted by the National Cancer Institute of the National Institutes of Health, as further amended and extended. As such, the United States government has certain rights in the invention of HuCC49*.

FIELD OF THE INVENTION

The present invention is directed to the fields of immunology and genetic engineering.

BACKGROUND OF THE INVENTION

The following information is provided for the purpose of making known information believed by the applicants to be of possible relevance to the present invention. No admission is necessarily intended, nor should be construed, that any of the following information constitutes prior art against the present invention.

Antibodies are specific immunoglobulin (Ig) polypeptides produced by the vertebrate immune system in response to challenges by foreign proteins, glyco-proteins, cells, or other antigenic foreign substances. The binding specificity of such polypeptides to a particular antigen is highly refined, with each antibody being almost exclusively directed to the particular antigen which elicited it.

Two major methods of generating vertebrate antibodies are presently utilized: generation in situ by the mammalian B lymphocytes and generation in cell culture by B cell hybrids. Antibodies are generated in situ as a result of the differentiation of immature B lymphocytes into plasma cells (see Gough (1981), Trends in Biochem Sci, 6:203). Even when only a single antigen is introduced into the immune system of a particular mammal, a uniform population of antibodies does not result, i.e., the response is polyclonal. The limited but inherent heterogeneity of polyclonal antibodies is overcome by the use of hybridoma technology to create “monoclonal” antibodies in cell cultures by B cell hybridomas (see Kohler and Milstein (1975), Nature, 256:495-497). In this process, a mammal is injected with an antigen, and its relatively short-lived, or mortal, splenocytes or lymphocytes are fused with an immortal tumor cell line. The fusion produces hybrid cells or “hybridomas” which are both immortal and capable of producing the genetically-coded antibody of the B cell.

In many applications, the use of monoclonal antibodies produced in non-human animals is severely restricted where the monoclonal antibodies are to be used in humans. Repeated injections in humans of a “foreign” antibody, such as a mouse antibody, may lead to harmful hypersensitivity reactions, i.e., an anti-idiotypic, or anti-mouse antibody (HAMA), response (see Shawler et al. (1985), Journal of Immunology, 135:1530-1535; and Sear et al., J. Biol. Resp. Modifiers, 3:138-150).

Various attempts have already been made to manufacture human-derived monoclonal antibodies by using human hybridomas (see Olsson et al. (1980), Proc. Natl. Acad. Sci. USA, 77:5429; and Roder et al. (1986), Methods in Enzymology, 121:140-167). Unfortunately, yields of monoclonal antibodies from human hybridoma cell lines are relatively low compared to mouse hybridomas. In addition, human cell lines expressing immunoglobulins are relatively unstable compared to mouse cell lines, and the antibody producing capability of these human cell lines is transient. Thus, while human immunoglobulins are highly desirable, human hybridoma techniques have not yet reached the stage where human monoclonal antibodies with required antigenic specificities can be easily obtained.

Thus, antibodies of nonhuman origin have been genetically engineered to create chimeric or humanized antibodies. Such genetic engineering results in antibodies with a reduced risk of a HAMA response compared to that expected after injection of a human patient with a mouse antibody. In a chimeric antibody, non-human regions of immunoglobulin constant sequences are replaced by corresponding human ones (see U.S. Pat. No. 4,816,567 to Cabilly et al., Genentech); in a humanized antibody, complementarity determining regions (CDRs) are grafted onto human framework regions (FR) (see European Patent Office Application (EPO) 0 239 400 to Winter). Some researchers have produced Fv antibodies (see U.S. Pat. No. 4,642,334 to Moore, DNAX) and single chain Fv (SCFV) antibodies (see U.S. Pat. No. 4,946,778 to Ladner, Genex).

The above patent publications only show the production of antibody fragments in which some portion of the variable domains is coded for by nonhuman V gene regions. Humanized antibodies to date still retain various portions of light and heavy chain variable regions of nonhuman origin: the chimeric, Fv and single chain Fv antibodies retain the entire variable region of nonhuman origin and CDR-grafted antibodies retain CDR of nonhuman origin.

Such nonhuman-derived regions are expected to elicit an immunogenic reaction when administered into a human patient (see Brüggemann et al. (1989), J. Exp. Med., 170:2153-2157; and Lo Buglio (1991), Sixth International Conference on Monoclonal Antibody Immunoconjugates for Cancer, San Diego, Calif.). Thus, it is most desirable to obtain a human variable region which is capable of binding to a selected antigen.

One known human carcinoma tumor antigen is tumor-associated glycoprotein-72 (TAG-72), as defined by monoclonal antibody B72.3 (see Thor et al. (1986) Cancer Res., 46:3118-3124; and Johnson, et al. (1986), Cancer Res., 46:850-857). TAG-72 is associated with the surface of certain tumor cells of human origin, specifically the LS174T tumor cell line (American Type Culture Collection (ATCC) No. CL 188), which is a variant of the LS180 (ATCC No. CL 187) colon adeno-carcinoma line.

Numerous murine monoclonal antibodies have been developed which have binding specificity for TAG-72. Exemplary murine monoclonal antibodies include the “CC” (colon cancer) monoclonal antibodies, which are a library of murine monoclonal antibodies developed using TAG-72 purified on an immunoaffinity column with an immobilized anti-TAG-72 antibody, B72.3 (ATCC HB-8108) (see EP 394277, to Schlom et al., National Cancer Institute). Certain CC antibodies were deposited with the ATCC: CC49 (ATCC No. HB 9459); CC83 (ATCC No. HB 9453); CC46 (ATCC No. HB 9458); CC92 (ATCC No. HB 9454); CC30 (ATCC NO. HB 9457); CC11 (ATCC No. 9455) and CC15 (ATCC No. HB 9460). Various antibodies of the CC series have been chimerized (see, for example, EPO 0 365 997 to Mezes et al., The Dow Chemical Company).

It is thus of great interest to develop antibodies against TAG-72 containing a light and/or heavy chain variable region(s) derived from human antibodies. However, the prior art simply does not teach recombinant and immunologic techniques capable of routinely producing an anti-TAG-72 antibody in which the light chain and/or the heavy chain variable regions have specificity and affinity for TAG-72 and which are derived from human sequences so as to elicit expectedly low or no HAMA response. It is known that the function of an immunoglobulin molecule is dependent on its three dimensional structure, which in turn is dependent on its primary amino acid sequence. A change of a few or even one amino acid can drastically affect the binding function of the antibody, i.e., the resultant antibodies are generally presumed to be a non-specific immunoglobulin (NSI), i.e., lacking in antibody character, (see, for example, U.S. Pat. No. 4,816,567 to Cabilly et al., Genentech).

Surprisingly, the present invention is capable of meeting many of these above mentioned needs and provides a method for supplying the desired antibodies. For example, in one aspect, the present invention provides a cell capable of expressing a composite antibody having binding specificity for TAG-72, said cell being transformed with (a) a DNA sequence encoding at least a portion of a light chain variable region (V_(L)) effectively homologous to the human Subgroup IV germline gene (Hum4 V_(L)); and a DNA sequence segment encoding at least a portion of a heavy chain variable region (V_(H)) capable of combining with the V_(L) into a three dimensional structure having the ability to bind to TAG-72. As is customary in the art, the term “Hum4 V_(L)” which is itself derived from the designation of a protein—i.e. the variable domain of a light chain belonging to Subgroup IV of the class of human κ light chains—can indicate this protein and/or the gene(s) or DNA sequence(s) which encode it.

In one aspect, the present invention concerns a composite antibody or antibody fragment comprising a DNA sequence encoding at least one chain which comprises a variable region having a heavy chain (V_(H)) and a light chain (V_(L)), (A) said V_(H) being encoded by a DNA sequence comprising a subsegment effectively homologous to the V_(H)αTAG germline gene (V_(H)αTAG), and (B) said V_(L) being encoded by a DNA sequence comprising a subsegment effectively homologous to the human Subgroup IV germline gene (Hum_(k)IV).

In another aspect, the present invention provides a composite antibody or antibody fragment having binding specificity for TAG-72, comprising (a) a DNA sequence encoding at least a portion of a light chain variable region (V_(L)) effectively homologous to the human Subgroup IV germline gene (Hum4 V_(L)); and a DNA sequence segment encoding at least a portion of a heavy chain variable region (V_(H)) capable of combining with the V_(L) into a three dimensional structure having the ability to bind TAG-72.

The invention further includes the aforementioned antibody alone or conjugated to an imaging marker or therapeutic agent. The invention also includes a composition comprising the aforementioned antibody in unconjugated or conjugated form in a pharmaceutically acceptable, non-toxic, sterile carrier.

The invention is also directed to a method for in vivo diagnosis of cancer which comprises administering to an animal containing a tumor expressing TAG-72 a pharmaceutically effective amount of the aforementioned composition for the in situ detection of carcinoma lesions.

The invention is also directed to a method for intraoperative therapy which comprises (a) administering to a patient containing a tumor expressing TAG-72 a pharmaceutically effective amount of the aforementioned composition, whereby the tumor is localized, and (b) excising the localized tumors.

Additionally, the invention also concerns a process for preparing and expressing a composite antibody. Some of these processes are as follows. A process which comprises transforming a cell with a DNA sequence encoding at least a portion of a light chain variable region (V_(L)) effectively homologous to the human Subgroup IV germline gene (Hum4 V_(L)); and a DNA sequence segment encoding at least a portion of a heavy chain variable region (V_(H)) which is capable of combining with the V_(L) to form a three dimensional structure having the ability to bind to TAG-72. A process for preparing a composite antibody or antibody which comprises culturing a cell containing a DNA sequence encoding at least a portion of a light chain variable region (V_(L)) effectively homologous to the human Subgroup IV germline gene (Hum4 V_(L)); and a DNA sequence segment encoding at least a portion of a heavy chain variable region (V_(H)) capable of combining with the V_(L) into a three dimensional structure having the ability to bind to TAG-72 under sufficient conditions for the cell to express the immunoglobulin light chain and immuno-globulin heavy chain. A process for preparing an antibody conjugate comprising contacting the aforementioned antibody or antibody with an imaging marker or therapeutic agent.

DESCRIPTION OF THE DRAWINGS

FIG. 1 illustrates a basic immunoglobulin structure.

FIG. 2, i.e. FIGS. 2A-2G illustrate the nucleotide sequences of V_(H)αTAG (SEQ ID NO:40), CC46 V_(H) (SEQ ID NO:41), CC49 V_(H) (SEQ ID NO:42), CC83 V_(H) (SEQ ID NO:43) and CC92 V_(H) (SEQ ID NO:44).

FIG. 3, i.e. FIGS. 3A-3E illustrate the amino acid sequences of V_(H)αTAG (SEQ ID NO:45), CC46 V_(H) (SEQ ID NO:46), CC49 V_(H) (SEQ ID NO:47), CC83 V_(H) (SEQ ID NO:48)and CC92 V_(H) (SEQ ID NO:49).

FIG. 4, i.e. FIGS. 4A-4B illustrate the V_(H) nucleotide (SEQ ID NO:50) and amino acid (SEQ ID NO:51) sequences of antibody B17X2.

FIG. 5, i.e. FIGS. 5A-5B illustrate the mouse germline J-H genes (SEQ ID NO:52) from pNP9.

FIG. 6 illustrates the plasmid map of p49 gl-2.3.

FIG. 7 illustrates the plasmid map of p83 gl-2.3.

FIG. 8, i.e. FIGS. 8A-8B illustrate the entire sequence of HUMVL(+)(SEQ ID NO:53) and HUMVL(−)(SEQ ID NO:54).

FIG. 9 illustrates the human J4 (HJ4) nucleotide sequence (SEQ ID NO:55) and amino acid sequence (SEQ ID NO:56). The underlined DNA sequence represents a part of the sequence used for the 3′ terminal PCR primer, HUMVL(−).

FIG. 10, i.e. FIGS. 10A-10E illustrate the nucleotide sequences (SEQ ID NO:57), and the amino acid sequences (SEQ ID NO:58) of Hum4 V_(L), ClaI-HindIII segment.

FIG. 11 illustrates a schematic representation of the human germline Subgroup IV V_(L) gene (Hum4 V_(L)), as the target for the PCR, referring to this gene as the “human germline Subgroup IV gene (Hum V_(L)).” The arrowheads shown on the PCR primers indicate the direction of synthesis.

FIG. 12 shows the results of an agarose gel electrophoresis of a PCR reaction to obtain the Hum4 V_(L) gene, as visualized by ethidium bromide under long wavelength UV light. Lane 1: λ HindIII standard; Lane 2: no Taq polymerase control; Lane 3: no primers added; Lane 4: no human DNA template; Lane 5: GeneAmp kit positive control; Lane 6: 3 μg human DNA with primers and Tag polymerase; Lane 7: same as Lane 6, but with 1 μg human DNA; Lane 8: φX174-HaeIII DNA standard.

FIG. 13, i.e. FIGS. 13A-13B illustrate the restriction enzyme maps of pRL1000, and precursor plasmids pSV2neo, pSV2neo-101 and pSV2neo-102. “X” indicates where the HindIII site of pSV2neo has been destroyed.

FIG. 14 illustrates a polylinker segment made by synthesizing two oligonucleotides: CH(+)(SEQ ID NO:59) and CH(−) (SEQ ID NO:60).

FIG. 15 illustrates a primer, NEO102SEQ (SEQ ID NO:61), used for sequencing plasmid DNA from several clones of pSV2neo-102. The primer is underlined; the boxed BamHI sequence indicates the site where the polylinker was inserted in pSV2neo-101.

FIG. 16 illustrates an autoradiogram depicting the DNA sequence of the polylinker region both in pSV2neo-102, where the (+) strand of the polylinker DNA is inserted in the plasmid's sense strand, and in pSV2neo-120, where the (−) strand of the polylinker DNA is inserted in the plasmid's sense strand.

FIG. 17 illustrates a partial nucleotide sequence segment (SEQ ID NO:62) of pRL1000, reading the (+) strand from the primer NEO102SEQ. Sequence data past the HindIII site is from the human Cκ HindIII-BamHI insert.

FIG. 18 illustrates the restriction enzyme map of pRL1001.

FIG. 19 illustrates an autoradiogram of DNA sequence for pRL1001 clones.

FIG. 20, i.e. FIGS. 20A-20C illustrate a competition assay for binding to TAG-using a composite Hum4 V_(L), V_(H)αTAG antibody.

FIG. 21 illustrates a general DNA construction of a single chain, composite Hum4 V_(L), V_(H)αTAG antibody.

FIG. 22, i.e. FIGS. 22A-22C illustrate the nucleotide sequence (SEQ ID NO:63) and amino acid sequence (SEQ ID NO:64) of SCFV1.

FIG. 23, i.e. FIGS. 23A-23B illustrate the construction of plasmid pCGS515/SCFV1.

FIG. 24, i.e. FIGS. 24A-24B show the construction of plasmid pSCFV31.

FIG. 25, i.e. FIGS. 25A-25B illustrate the construction of E. coli SCFV expression plasmids containing Hum4 V_(L).

FIG. 26, i.e. FIGS. 26A-26D show the DNA sequence (SEQ ID NO:65) and amino acid sequence (SEQ ID NO:66) of Hum4 V_(L)-CC49V_(H) SCFV present in pSCFVUHH.

FIG. 27 shows the construction of plasmid pSCFV UHH and a schematic of a combinatorial library of V_(H) genes with Hum4 V_(L).

FIG. 28, i.e. FIGS. 28A-28C illustrate the nucleotide sequence (SEQ ID NO:67) of FLAG peptide adapter (SEQ ID NO:68) in pATDFLAG.

FIG. 29 illustrates the construction of pATDFLAG, pHumVL-HumVH (X) and pSC49FLAG.

FIG. 30, i.e. FIGS. 30A-30D illustrate the nucleotide (SEQ ID NO:69) and amino acid (SEQ ID NO:70) sequences of pSC49FLAG.

FIG. 31, i.e. FIGS. 31A-31B show the flow diagram for the discovery of HUM4 VL-VH combinations that compete with prototype TAG-binding antibodies or mimetics.

FIG. 32, i.e. FIGS. 32A-32B illustrate the “humanization” protocols used in Example 6 to produce the humanized antibody variable regions derived from CC49. FIG. 32A presents the amino acid sequences of the native CC49 V_(L) (SEQ ID NO:71), the V_(L) framework regions (SEQ ID NO:72) of human MAb LEN, and the humanized version of CC49 V_(L) (SEQ ID NO:73) (HuCC49) derived from nCC49 and LEN. FIG. 32B presents the amino acid sequences of the native CC49 V_(H) (SEQ ID NO:74), the V_(H) framework regions (SEQ ID NO:75) of human MAb 21/28′CL, and the humanized version of CC49 V_(H) (SEQ ID NO:76) (HuCC49) derived from nCC49 and 21/28′CL. Framework residues deemed important for maintaining the combining-site structure of CC49, as based on the known structures of the antigen-binding regions of McPC603 (for V_(L)) and 36-71 (for V_(H) ), are marked with an asterisk.

FIG. 33, i.e. FIGS. 33A-33B illustrate the nucleotide sequences of the humanized CC49 (HuCC49*) V_(L) (SEQ ID NO:77) and V_(H) (SEQ ID NO:78) variable regions genes, respectively, at nucleotides 74-412 and nucleotides 70-415. Lowercase letters indicate sequences that do not encode the variable region domains or their leader peptides.

FIG. 34, i.e. FIGS. 34A-34B present a schematic illustration of the process used in Example 6 to form the eukaryotic expression constructs of the humanized light (A) and heavy (B) chains of HuCC49*. Thin lines represent sequences derived from the prokaryotic vectors pBR322, pBluescript SK⁺, or pCR II. Thick lines depict human κ or γ1 constant regions. Densely hatched boxes represent the humanized variable regions, while sparsely hatched boxes indicate murine variable regions. Boxes with vertical, horizontal, or vertical-horizontal cross bars show neomycin, mycophenolic acid, or hygromycin resistance genes, and thin arrows show their transcriptional direction.

FIG. 35, i.e. FIGS. 35A-35B illustrate SDS-PAGE analyses of purified HuCC49* (1) and cCC49 (2) under non-reducing (A) and reducing (B) conditions. Numbers beneath the “M” represent sizes of molecular weight markers.

FIG. 36, i.e. FIGS. 36A-36B illustrate HPCL analyses of (A) radioiodinated HuCC49* (¹³¹I-labeled) and (B) radioiodinated cCC49 (¹²⁵I-labeled) MAbs.

FIG. 37 shows the reactivity of HuCC49* (closed circles), cCC49 (open circles), and nCC49 (closed squares) in a competition RIA against ¹²⁵I-labeled nCC49 bound to BSM-immobilized TAG-72. The controls were human IgG (closed triangles) and MOPC-21 (open triangles).

FIG. 38 shows the clearance of radioiodinated HuCC49* and cCC49 MAbs from the serum of mice.

DETAILED DESCRIPTION OF THE INVENTION

Prior to setting forth the invention, definitions of certain terms which are used in this disclosure are set forth below:

Antibody—This refers to single chain, two-chain, and multi-chain proteins and glycoproteins belonging to the classes of polyclonal, monoclonal, chimeric, and hetero immunoglobulins (monoclonal antibodies being preferred); it also includes synthetic and genetically engineered variants of these immunoglobulins. “Antibody fragment” includes Fab, Fab′, F(ab′)₂, and Fv fragments, as well as any portion of an antibody having specificity toward a desired target epitope or epitopes.

Humanized antibody—This will refer to an antibody derived from a non-human antibody, typically murine, that retains or substantially retains the antigen-binding proper-ties of the parent antibody but which is less immunogenic in humans. This may be achieved by various methods including (a) grafting only the non-human CDRs onto human framework and constant regions with or without retention of critical framework residues, or (b) transplanting the entire non-human variable domains, but “cloaking” them with a human-like section by replacement of surface residues. Such methods as are useful in practicing the present invention include those disclosed in Jones et al., Morrison et al., Proc. Natl. Acad. Sci. USA, 81:6851-6855 (1984); Morrison and Oi, Adv. Immunol., 44:65-92 (1988); Verhoeyen et al., Science, 239:1534-1536 (1988); Padlan, Molec. Immun., 28:489-498 (1991); Padlan, Molec. Immun., 31(3):169-217 (1994).

Complementarity Determining Region, or CDR—The term CDR, as used herein, refers to amino acid sequences which together define the binding affinity and specificity of the natural Fv region of a native immunoglobulin binding site as delineated by Kabat et al (1991).

Framework Region—The term FR, as used herein, refers to amino acid sequences interposed between CDRs. These portions of the antibody serve to hold the CDRs in an appropriate orientation for antigen binding.

Constant Region—The portion of the antibody molecule which confers effector functions. In the present invention, murine constant regions are substituted with human constant regions. The constant regions of the subject chimeric or humanized antibodies are derived from human immunoglobulins. The heavy chain constant region can be selected from any of the five isotypes: alpha, delta, epsilon, gamma or mu. Further, heavy chains of various subclasses (such as the IgG subclasses of heavy chains) are responsible for different effector functions and thus, by choosing the desired heavy chain constant region chimeric antibodies with desired effector function can be produced. Preferred constant regions are gamma 1 (IgG1), gamma 3 (IgG3) and gamma 4 (IgG4). More preferred is a constant region of the gamma 1 (IgG1) isotype. The light chain constant region can be of the kappa or lambda type, preferably of the kappa type.

Chimeric antibody—This is an antibody containing sequences derived from two different antibodies, which typically are of different species. Most typically chimeric antibodies comprise human and murine antibody fragments, generally human constant and murine variable regions.

Mammals—Animals that nourish their young with milk secreted by mammary glands, preferably warm blooded mammals, more preferably humans.

Immunogenicity—A measure of the ability of a targeting protein or therapeutic moiety to elicit an immune response (humoral or cellular) when administered to a recipient. The present invention is concerned with the immunogenicity of the subject humanized antibodies or fragments thereof.

Humanized antibody of reduced immunogenicity—This refers to a humanized antibody exhibiting reduced immunogenicity relative to the parent antibody.

Humanized antibody substantially retaining the binding properties of the parent antibody—This refers to a humanized antibody which retains the ability to specifically bind the antigen recognized by the parent antibody used to produce such humanized antibodies. Preferably the humanized antibody will exhibit the same or substantially the same antigen-binding affinity and avidity as the parent antibody, e.g., CC49. Preferably, the affinity of the antibody will at least about 10% of that of the parent antibody. More preferably, the affinity will be at least about 25%, i.e. at least two-fold less than the affinity of the parent antibody. Most preferably the affinity will be at least about 50% that of the parent antibody. Methods for assaying antigen-binding affinity are well known in the art and include half-maximal binding assays, competition assays, and Scatchard analysis. Suitable antigen binding assays are described in this application.

In a preferred embodiment, the antibodies and fragments of the present invention will be substantially homologous with those exemplified below and/or presented in the Figures. The phrase “substantially homologous” is used in regard to the similarity of a subject amino acid sequence (of an oligo- or poly-peptide or protein) to a related, reference amino acid sequence. This phrase is defined as at least about 75% “correspondence”—i.e. the state of identical amino acid residues being situated in parallel—between the subject and reference sequences when those sequences are in “alignment,” i.e. when a minimal number of “null” bases have been inserted in the subject and/or reference sequences so as to maximize the number of existing bases in correspondence between the sequences. “Null” bases are not part of the subject and reference sequences; also, the minimal number of “null” bases inserted in the subject sequence may differ from the minimal number inserted in the reference sequence. In this definition, a reference sequence is considered “related” to a subject sequence where both amino acid sequences make up proteins or portions of proteins which are either αTAG antibodies or antibody fragments with αTAG binding affinity. Each of the proteins comprising these αTAG antibodies or antibody fragments may independently be antibodies or antibody fragments or bi- or multi-functional proteins, e.g., such as fusion proteins, bi- and multi-specific antibodies, single chain antibodies, and the like.

Nucleic acids, amino acids, peptides, protective groups, active groups and so on, when abbreviated, are abbreviated according to the IUPAC IUB (Commission on Biological Nomenclature) or the practice in the fields concerned.

The basic immunoglobulin structural unit is set forth in FIG. 1. The terms “constant” and “variable” are used functionally. The variable regions of both light (V_(L)) and heavy (V_(H)) chains determine binding recognition and specificity to the antigen. The constant region domains of light (C_(L)) and heavy (C_(H)) chains confer important biological properties such as antibody chain association, secretion, transplacental mobility, complement binding, binding to Fc receptors and the like.

The immunoglobulins of this invention have been developed to address the problems of the prior art. The methods of this invention produce, and the invention is directed to, composite antibodies. By “composite antibodies” is meant immunoglobulins comprising variable regions not hitherto found associated with each other in nature. By “composite Hum4 V_(L), V_(H) antibody” means an antibody or immunoreactive fragment thereof which is characterized by having at least a portion of the V_(L) region encoded by DNA derived from the Hum4 V_(L) germline gene and at least a portion of a V_(H) region capable of combining with the V_(L) to form a three dimensional structure having the ability to bind to TAG-72.

The composite Hum4 V_(L), V_(H) antibodies of the present invention assume a conformation having an antigen binding site which binds specifically and with sufficient strength to TAG-72 to form a complex capable of being isolated by using standard assay techniques (e.g., enzyme-linked immunosorbent assay (ELISA), radioimmunoassay (RIA), or flourescence-activated cell sorter analysis (FACS), immunohistochemistry and the like). Preferably, the composite Hum4 V_(L), V_(H) antibodies of the present invention have an antigen binding affinity or avidity greater than 10⁵ M⁻¹, more preferably greater than 10⁶ M⁻¹ and most preferably greater than 10⁸ M^(−l). For a discussion of the techniques for generating and reviewing immunoglobulin binding affinities see Munson (1983), Methods Enzymol., 92:543-577 and Scatchard (1949), Ann. N.Y. Acad. Sci., 51:660-672.

Human antibody kappa chains have been classified into four subgroups on the basis of invariant amino acid sequences (see, for example, Kabat et al. (1991), Sequences of Proteins of Immunological Interest (4th ed.), published by The U.S. Department of Health and Human Services). There appear to be approximately 80 human V_(K) genes, but only one Subgroup IV V_(K) gene has been identified in the human genome (see Klobeck, et al. (1985), Nucleic Acids Research, 13:6516-6528). The nucleotide sequence of Hum4 V_(L) is set forth in Kabat et al. (1991), supra.

It has been found, quite surprisingly, that an immunoglobulin having a light chain with at least a portion of the V_(L) encoded by a gene derived from Hum4 V_(L) may, if combined with a suitable V_(H), have binding specificity for TAG-72. The type of J_(L) gene segment selected is not critical to the invention, in that it is expected that any J_(L), if present, can associate with the Hum4 V_(L). The present invention obviously contemplates the Hum4 V_(L) in association with a human J_(k) sequence. The five human J_(k) sequences are set forth in Heiter et al. (1982), The Journal of Biological Chemistry, 357:1516-1522. However, the present invention is not intended to be limited to the human J_(k). The present invention specifically contemplates the Hum4 V_(L) in association with any of the at least six human J_(l) genes (see. Hollis et al. (1982), Nature, 296:321-325).

An exemplary technique for engineering the Hum4 V_(L) with selected J_(L) segments includes synthesizing a primer having a so-called “wagging tail”, that does not hybridize with the target DNA; thereafter, the sequences are amplified and spliced together by overlap extension (see Horton et al. (1989), Gene, 77:61-68).

The C_(L) of the composite Hum4 V_(L), V_(H) antibodies is not critical to the invention. To date, the Hum4 V_(L) has only been reported as having been naturally rearranged with the single C_(k) gene (see Heiter et al. (1980), Cell, 22:197-207). However, the present invention is not intended to be limited to the C_(k) light chain constant domain. That is, the C_(L) gene segment may also be any of the at least six C_(l) genes (see Hollis et al., supra).

The DNA encoding the heavy chain variable region consists roughly of a heavy chain variable (V_(H)) gene sequence, a heavy chain diversity (D_(H)) gene sequence, and a heavy chain joining (J_(H)) gene sequence.

The present invention is directed to any V_(H) capable of combining with a light chain variable region effectively homologous to the light chain variable region encoded by the human Subgroup IV germline gene, to form a three dimensional structure having the ability to bind to TAG-72.

The choice of D_(H) and J_(H) segment of the composite Hum4 V_(L), V_(H) antibody are not critical to the present invention. Obviously, human and murine D_(H) and J_(H) gene segments are contemplated, provided that a given combination does not significantly decrease binding to TAG-72. Specifically, when utilizing CC46 V_(H), CC49 V_(H), CC83 V_(H) and CC92 V_(H), the composite Hum4 V_(L), V_(H) antibody will be designed to utilize the D_(H) and J_(H) segments which naturally associated with those V_(H) of the respective hybridomas (see FIGS. 2A-G and 3A-E). Exemplary murine and human D_(H) and J_(H) sequences are set forth in Kabat et al. (1991), supra. An exemplary technique for engineering such selected D_(H) and J_(H) segments with a V_(H) sequence of choice includes synthesizing selected oligonucleotides, annealing and ligating in a cloning procedure (see, Horton et al., supra).

In a specific embodiment the composite Hum4 V_(L), V_(H) antibody will be a “composite Hum4 V_(L), V_(H)αTAG antibody”, means an antibody or immunoreactive fragment thereof which is characterized by having at least a portion of the V_(L) region encoded by DNA derived from the Hum4 V_(L) germline gene and at least a portion of the V_(H) region encoded by DNA derived from the V_(H)αTAG germline gene, which is known in the art (see, for example, EPO 0 365 997 to Mezes et al., the Dow Chemical Company). FIGS. 2A-G show the nucleotide sequence of V_(H)αTAG, and the nucleotide sequences encoding the V_(H) of the CC46, CC49, CC83 and CC92 antibodies, respectively. FIG. 3 shows the corresponding amino acid sequences of V_(H)αTAG, CC46 V_(H), CC49 V_(H), CC83 V_(H) and CC92 V_(H).

A comparison of the nucleotide and amino acid sequences of V_(H)αTAG, CC46 V_(H), CC49 V_(H), CC83 V_(H) and CC92 V_(H) shows that those CC antibodies are derived from V_(H)αTAG. Somatic mutations occurring during productive rearrangement of the V_(H) derived from V_(H)αTAG in a B cell gave rise to some nucleotide changes that may or may not result in a homologous amino acid change between the productively rearranged hybridomas (see, EPO 0 365 997).

Because the nucleotide sequences of the V_(H)αTAG and Hum4 V_(L) germline genes have been provided herein, the present invention is intended to include other antibody genes which are productively rearranged from the V_(H)αTAG germline gene. Other antibodies encoded by DNA derived from V_(H)αTAG may be identified by using a hybridization probe made from the DNA or RNA of the V_(H)αTAG or rearranged genes containing the recombined V_(H)αTAG. Specifically, the probe will include of all or a part of the V_(H)αTAG germline gene and its flanking regions. By “flanking regions” is meant to include those DNA sequences from the 5′ end of the V_(H)αTAG to the 3′ end of the upstream gene, and from 3′ end of the V_(H)αTAG to the 5′ end of the downstream gene.

The CDR from the variable region of antibodies derived from V_(H)αTAG may be grafted onto the FR of selected V_(H), i.e., FR of a human antibody (see EPO 0 239 400 to Winter). For example, the cell line, B17X2, expresses an antibody utilizing a variable light chain encoded by a gene derived from Hum4 V_(L) and a variable heavy chain which makes a stable V_(L) and V_(H) combination (see Marsh et al. (1985), Nucleic Acids Research, 13:6531-6544; and Polke et al. (1982), Immunobiol. 163:95-109. The nucleotide sequence of the V_(H) chain for B17X2 is shown in FIG. 4. The B17X2 cell line is publicly available from Dr. Christine Polke, Universitäts-Kinderklinik, Josef-Schneider-Str. 2, 8700 Würzburg, FRG). B17X2 is directed to N-Acetyl-D-Glucosamine and is not specific for TAG-72.

However, consensus sequences of antibody derived from the CDR1 of V_(H)αTAG (amino acid residues 31 to 35 of FIGS. 3A-E) may be inserted into B17X2 (amino acid residues 31 to 37 of FIGS. 4A-B) and the CDR2 of V_(H)αTAG (amino residues 50 to 65 of FIGS. 3A-E) may be inserted into B17X2 (amino acid residues 52 to 67 of FIGS. 4A-B). The CDR3 may be replaced by any D_(H) and J_(H) sequence which does not affect the binding of the antibody for TAG-72 but, specifically, may be replaced by the CDR3 of an antibody having its V_(H) derived from V_(H)αTAG, e.g., CC46, CC49, CC83 and CC92. Exemplary techniques for such replacement are set forth in Horton et al., supra.

The C_(H) domains of immunoglobulin heavy chain derived from V_(H)αTAG genes, for example may be changed to a human sequence by known techniques (see, U.S. Pat. No. 4,816,567 to Cabilly, Genentech). C_(H) domains may be of various complete or shortened human isotypes, i.e., IgG (e.g., IgG₁, IgG₂, IgG₃, and IgG₄), IgA (e.g., IgA1 and IgA2), IgD, IgE, IgM, as well as the various allotypes of the individual groups (see Kabat et al. (1991), supra).

Given the teachings of the present invention, it should be apparent to the skilled artisan that human V_(H) genes can be tested for their ability to produce an anti-TAG-72 immunoglobilin combination with the Hum4 V_(L) gene. The V_(L) may be used to isolate a gene encoding for a V_(H) having the ability to bind to TAG-72 to test myriad combinations of Hum4 V_(L) and V_(H) that may not naturally occur in nature, e.g., by generating a combinatorial library using the Hum4 V_(L) gene to select a suitable V_(H). Examples of these enabling technologies include screening of combinatorial libraries of V_(L)-V_(H) combinations using an Fab or single chain antibody (SCFV) format expressed on the surfaces of fd phage (Clackson, et al. (1991), Nature, 352:624-628), or using a l phage system for expression of Fv's or Fabs (Huse, et al. (1989), Science, 246:1275-1281). However, according to the teachings set forth herein, it is now possible to clone SCFV antibodies in E. coli, and express the SCFVs as secreted soluble proteins. SCFV proteins produced in E. coli that contain a Hum4 V_(L) gene can be screened for binding to TAG-72 using, for example, a two-membrane filter screening system (Skerra, et al. (1991), Analytical Biochemistry, 196:151-155).

The desired gene repertoire can be isolated from human genetic material obtained from any suitable source, e.g., peripheral blood lymphocytes, spleen cells and lymph nodes of a patient with tumor expressing TAG-72. In some cases, it is desirable to bias the repertoire for a preselected activity, such as by using as a source of nucleic acid, cells (source cells) from vertebrates in any one of various stages of age, health and immune response.

Cells coding for the desired sequence may be isolated, and genomic DNA fragmented by one or more restriction enzymes. Tissue (e.g., primary and secondary lymph organs, neoplastic tissue, white blood cells from peripheral blood and hybridomas) from an animal exposed to TAG-72 may be probed for selected antibody producing B cells. Variability among B cells derived from a common germline gene may result from somatic mutations occurring during productive rearrangement.

Generally, a probe made from the genomic DNA of a germline gene or rearranged gene can be used by those skilled in the art to find homologous sequences from unknown cells. For example, sequence information obtained from Hum4 V_(L) and V_(H)αTAG may be used to generate hybridization probes for naturally-occurring rearranged V regions, including the 5′ and 3′ nontranslated flanking regions. The genomic DNA may include naturally-occurring introns for portions thereof, provided that functional splice donor and splice acceptor regions had been present in the case of mammalian cell sources.

Additionally, the DNA may also be obtained from a cDNA library. mRNA coding for heavy or light chain variable domain may be isolated from a suitable source, either mature B cells or a hybridoma culture, employing standard techniques of RNA isolation. The DNA or amino acids also may be synthetically synthesized and constructed by standard techniques of annealing and ligating fragments (see Jones, et al. (1986), Nature, 321:522-525; Reichmann et al., (1988), Nature, 332:323-327; Sambrook et al. (1989), supra and Merrifield et al. (1963), J. Amer. Chem. Soc., 85:2149-2154). Heavy and light chains may be combined in vitro to gain antibody activity (see Edelman, et al. (1963), Proc. Natl. Acad. Sci. USA, 50:753).

The present invention also contemplates a gene library of V_(H)αTAG homologs, preferably human homologs of V_(H)αTAG. By “homolog” is meant a gene coding for a V_(H) region (not necessarily derived from, or even effectively homologous to, the V_(H)αTAG germline gene) capable of combining with a light chain variable region effectively homologous to the light chain variable region encoded by the human Subgroup IV germline gene, to form a three dimensional structure having the ability to bind to TAG-72.

Preferably, the gene library is produced by a primer extension reaction or combination of primer extension reactions as described herein. The V_(H)αTAG homologs are preferably in an isolated form, that is, substantially free of materials such as, for example, primer extension reaction agents and/or substrates, genomic DNA segments, and the like. The present invention thus is directed to cloning the V_(H)αTAG-coding DNA homologs from a repertoire comprised of polynucleotide coding strands, such as genomic material containing the gene expressing the variable region or the messenger RNA (mRNA) which represents a transcript of the variable region. Nucleic acids coding for V_(H)αTAG-coding homologs can be derived from cells producing IgA, IgD, IgE, IgG or IgM, most preferably from IgM and IgG, producing cells.

The V_(H)αTAG-coding DNA homologs may be produced by primer extension. The term “primer” as used herein refers to a polynucleotide whether purified from a nucleic acid restriction digest or produced synthetically, which is capable of acting as a point of initiation of synthesis when placed under conditions in which synthesis of a primer extension product which is complimentary to a nucleic acid strand is induced, i.e., in the presence of nucleotides and an agent for polymer-ization such as DNA polymerase, reverse transcriptase and the like, and at a suitable temperature and pH.

Preferably, the V_(H)αTAG-coding DNA homologs may be produced by polymerase chain reaction (PCR) amplifi-cation of double stranded genomic or cDNA, wherein two primers are used for each coding strand of nucleic acid to be exponentially amplified. The first primer becomes part of the nonsense (minus or complementary) strand and hybridizes to a nucleotide sequence conserved among V_(H) (plus) strands within the repertoire. PCR is described in Mullis et al. (1987), Meth. Enz., 155:335-350; and PCR Technology, Erlich (ed.) (1989). PCR amplification of the mRNA from antibody-producing cells is set forth in Orlandi et al. (1989), Proc. Natl. Acad. Sci., USA, 86:3387-3837.

According to a preferred method, the V_(H)αTAG-coding DNA homologs are connected via linker to form a SCFV having a three dimensional structure capable of binding TAG-72. The SCFV construct can be in a V_(L)-L-V_(H) or V_(H)-L-V_(L) configuration. For a discussion of SCFV see Bird et al. (1988), Science, 242:423-426. The design of suitable peptide linker regions is described in U.S. Pat. No. 4,704,692 to Ladner et al., Genex.

The nucleotide sequence of a primer is selected to hybridize with a plurality of immunoglobulin heavy chain genes at a site substantially adjacent to the V_(H)αTAG-coding DNA homolog so that a nucleotide sequence coding for a functional (capable of binding) polypeptide is obtained. The choice of a primer's nucleotide sequence depends on factors such as the distance on the nucleic acid from the region coding for the desired receptor, its hybridization site on the nucleic acid relative to any second primer to be used, the number of genes in the repertoire it is to hybridize to, and the like. To hybridize to a plurality of different nucleic acid strands of V_(H)αTAG-coding DNA homolog, the primer must be a substantial complement of a nucleotide sequence conserved among the different strands.

The peptide linker may be coded for by the nucleic acid sequences that are part of the poly-nucleotide primers used to prepare the various gene libraries. The nucleic acid sequence coding for the peptide linker can be made up of nucleic acids attached to one of the primers or the nucleic acid sequence coding for the peptide linker may be derived from nucleic acid sequences that are attached to several polynucleotide primers used to create the gene libraries. Additionally, noncomplementary bases or longer sequences can be interspersed into the primer, provided the primer sequence has sufficient complemen-tarily with the sequence of the strand to be synthesized or amplified to non-randomly hybridize therewith and thereby form an extension product under polynucleotide synthesizing conditions (see Horton et al. (1989), Gene, 77:61-68).

Exemplary human V_(H) sequences from which complementary primers may be synthesized are set forth in Kabat et al. (1991), supra; Humphries et al. (1988), Nature, 331:446-449; Schroeder et al. (1990), Proc. Natl. Acad. Sci. USA, 87:6146-6150; Berman et al. (1988), EMBO Journal, 7:727-738; Lee et al. (1987), J. Mol. Biol., 195:761-768); Marks et al. (1991), Eur. J. Immunol., 21:985-991; Willems, et al. (1991), J. Immunol., 146:3646-3651; and Person et al. (1991), Proc Natl. Acad. Sci. USA, 88:2432-2436. To produce V_(H) coding DNA homologs, first primers are therefore chosen to hybridize to (i.e. be complementary to) conserved regions within the J region, CH1 region, hinge region, CH2 region, or CH3 region of immunoglobulin genes and the like. Second primers are therefore chosen to hydribidize with a conserved nucleotide sequence at the 5′ end of the V_(H)αTAG-coding DNA homolog such as in that area coding for the leader or first framework region.

Alternatively, the nucleic acid sequences coding for the peptide linker may be designed as part of a suitable vector. As used herein, the term “expression vector” refers to a nucleic acid molecule capable of directing the expression of genes to which they are operatively linked. The choice of vector to which a V_(H)αTAG-coding DNA homologs is operatively linked depends directly, as is well known in the art, on the functional properties desired, e.g., replication or protein expression, and the host cell (either procaryotic or eucaryotic) to be transformed, these being limitations inherent in the art of constructing recombinant DNA molecules. In preferred embodiments, the eucaryotic cell expression vectors used include a selection marker that is effective in an eucaryotic cell, preferably a drug resistant selection marker.

Expression vectors compatible with procaryotic cells are well known in the art and are available from several commercial sources. Typical of vector plasmids suitable for procaryotic cells are pUC8, pUC9, pBR322, and pBR329 available from BioRad Laboratories, (Richmond, Calif.), and pPL and pKK223 available from Pharmacia, (Piscataway, N.J.).

Expression vectors compatible with eucaryotic cells, preferably those compatible with vertebrate cells, can also be used. Eucaryotic cell expression vectors are well known in the art and are available from several commercial sources. Typically, such vectors are provided containing convenient restriction sites for insertion of the desired DNA homologue. Typical of vector plasmids suitable for eucaryotic cells are pSV2neo and pSV2gpt (ATCC), pSVL and pKSV-10 (Pharmacia), pBPV-1PML2d (International Biotechnologies, Inc.), and pTDT1 (ATCC).

The use of viral expression vectors to express the genes of the V_(H)αTAG-coding DNA homologs is also contemplated. As used herein, the term “viral expression vector” refers to a DNA molecule that includes a promoter sequences derived from the long terminal repeat (LTR) region of a viral genome. Exemplary phage include l phage and fd phage (see, Sambrook, et al. (1989), Molecular Cloning: A Laboratory Manual, (2nd ed.), and McCafferty et al. (1990), Nature, 6301:552-554. The population of V_(H)αTAG-coding DNA homologs and vectors are then cleaved with an endonuclease at shared restriction sites. A variety of methods have been developed to operatively link DNA to vectors via complementary cohesive termini. For instance, complementary cohesive termini can be engineered into the V_(H)αTAG-coding DNA homologs during the primer extension reaction by use of an appropriately designed polynucleotide synthesis primer, as previously discussed. The complementary cohesive termini of the vector and the DNA homolog are then operatively linked (ligated) to produce a unitary double stranded DNA molecule.

The restriction fragments of Hum4 V_(L)-coding DNA and the V_(H)αTAG-coding DNA homologs population are randomly ligated to the cleaved vector. A diverse, random population is produced with each vector having a V_(H)αTAG-coding DNA homolog and Hum4 V_(L)-coding DNA located in the same reading frame and under the control of the vector's promoter.

The resulting single chain construct is then introduced into an appropriate host to provide amplification and/or expression of a composite Hum4 V_(L), V_(H)αTAG homolog single chain antibody. Transformation of appropriate cell hosts with a recombinant DNA molecule of the present invention is accomplished by methods that typically depend on the type of vector used. With regard to transformation of procaryotic host cells, see, for example, Cohen et al. (1972), Proceedings National Academy of Science, USA, 69:2110; and Sambrook, et al. (1989), supra. With regard to the transformation of vertebrate cells with retroviral vectors containing rDNAs, see for example, Sorge et al. (1984), Mol. Cell. Biol., 4:1730-1737; Graham et al. (1973), Virol., 52:456; and Wigler et al. (1979), Proceedings National Academy of Sciences, USA,76:1373-1376.

Exemplary prokaryotic strains that may be used as hosts include E. coli, Bacilli, and other entero-bacteriaceae such as Salmonella typhimurium, and various Pseudomonas. Common eukaryotic microbes include S. cerevisiae and Pichia pastoris. Common higher eukaryotic host cells include Sp2/0, VERO and HeLa cells, Chinese hamster ovary (CHO) cell lines, and W138, BHK, COS-7 and MDCK cell lines. Furthermore, it is now also evident that any cell line producing Hum4 V_(L), e.g., the B17X2 human cell line, can be used as a recipient human cell line for introduction of a V_(H) gene complementary to the Hum4 V_(L) which allows binding to TAG-72. For example, the B17X2 heavy chain may be genetically modified to not produce the endogenous heavy chain by well known methods; in this way, glycosylation patterns of the antibody produced would be human and not non-human derived.

Successfully transformed cells, i.e., cells containing a gene encoding a composite Hum4 V_(L), V_(H)αTAG homolog single chain antibody operatively linked to a vector, can be identified by any suitable well known technique for detecting the binding of a receptor to a ligand. Preferred screening assays are those where the binding of the composite Hum4 V_(L), V_(H)αTAG homolog single chain antibody to TAG-72 produces a detectable signal, either directly or indirectly. Screening for productive Hum4 V_(L) and V_(H)αTAG homolog combinations, or in other words, testing for effective antigen binding sites to TAG-72 is possible by using for example, a radiolabeled or biotinylated screening agent, e.g., antigens, anti-bodies (e.g., B72.3, CC49, CC83, CC46, CC92, CC30, CC11 and CC15) or anti-idiotypic antibodies (see Huse et al., supra, and Sambrook et al., supra); or the use of marker peptides to the NH₂- or COOH-terminus of the SCFV construct (see Hopp et al. (1988), Biotechnology, 6:1204-1210).

Of course, the Hum4 V_(L)-coding DNA and the V_(H)αTAG-coding DNA homologs may be expressed as individual polypeptide chains (e.g., Fv) or with whole or fragmented constant regions (e.g., Fab, and F(ab′)₂). Accordingly, the Hum4 V_(L)-coding DNA and the V_(H)αTAG-coding DNA homologs may be individually inserted into a vector containing a C_(L) or C_(H) or fragment thereof, respectively. For a teaching of how to prepare suitable vectors see EPO 0 365 997 to Mezes et al., The Dow Chemical Company.

DNA sequences encoding the light chain and heavy chain of the composite Hum4 V_(L), V_(H) antibody may be inserted into separate expression vehicles, or into the same expression vehicle. When coexpressed within the same organism, either on the same or the different vectors, a functionally active Fv is produced. When the V_(H)αTAG-coding DNA homolog and Hum4 V_(L) polypeptides are expressed in different organisms, the respective polypeptides are isolated and then combined in an appropriate medium to form a Fv. See Greene et al., Methods in Molecular Biology, Vol. 9, Wickner et al. (ed.); and Sambrook et al., supra).

Subsequent recombinations can be effected through cleavage and removal of the Hum4 V_(L)-coding DNA sequence to use the V_(H)αTAG-coding DNA homologs to produce Hum4 V_(L)-coding DNA homologs. To produce a Hum4 V_(L)-coding DNA homolog, first primers are chosen to hybridize with (i.e. be complementary to) a conserved region within the J region or constant region of immunoglobulin light chain genes and the like. Second primers become part of the coding (plus) strand and hybridize to a nucleotide sequence conserved among minus strands. Hum4 V_(L)-coding DNA homologs are ligated into the vector containing the V_(H)αTAG-coding DNA homolog, thereby creating a second population of expression vectors. The present invention thus is directed to cloning the Hum4 V_(L)-coding DNA homologs from a repertoire comprised of polynucleotide coding strands, such as genomic material containing the gene expressing the variable region or the messenger RNA (mRNA) which represents a transcript of the variable region. It is thus possible to use an iterative process to define yet further, composite antibodies, using later generation V_(H)αTAG-coding DNA homologs and Hum4 V_(L)-coding DNA homologs.

The present invention further contemplates genetically modifying the antibody variable and constant regions to include effectively homologous variable region and constant region amino acid sequences. Generally, changes in the variable region will be made in order to improve or otherwise modify antigen binding properties of the receptor. Changes in the constant region of the antibody will, in general, be made in order to improve or otherwise modify biological properties, such as complement fixation, interaction with membranes, and other effector functions.

“Effectively homologous” refers to the concept that differences in the primary structure of the variable region may not alter the binding characteristics of the antibody. Normally, a DNA sequence is effectively homologous to a second DNA sequence if at least 70 percent, preferably at least 80 percent, and most preferably at least 90 percent of the active portions of the DNA sequence are homologous. Such changes are permissable in effectively homologous amino acid sequences so long as the resultant antibody retains its desired property.

If there is only a conservative difference between homologous positions of sequences, they can be regarded as equivalents under certain circumstances. General categories of potentially equivalent amino acids are set forth below, wherein amino acids within a group may be substituted for other amino acids in that group: (1) glutamic acid and aspartic acid; (2) hydrophobic amino acids such as alanine, valine, leucine and isoleucine; (3) asparagine and glutamine; (4) lysine and arginine and (5) threonine and serine.

Exemplary techniques for nucleotide replacement include the addition, deletion or substitution of various nucleotides, provided that the proper reading frame is maintained. Exemplary techniques include using polynucleotide-mediated, site-directed mutagenesis, i.e., using a single strand as a template for extension of the oligonucleotide to produce a strand containing the mutation (see Zoller et al. (1982), Nuc. Acids Res., 10:6487-6500; Norris et al. (1983), Nuc. Acids Res., 11:5103-5112; Zoller et al. (1984), DNA, 3:479-488; and Kramer et al. (1982), Nuc. Acids Res., 10:6475-6485) and polymerase chain reaction exponentially amplifying DNA in vitro using sequence specified oligo-nucleotides to incorporate selected changes (see PCR Technology: Principles and Applications for DNA Amplification, Erlich, (ed.) (1989); and Horton et al., supra).

Further, the antibodies may have their constant region domain modified, ie., the C_(L), CH₁, hinge, CH₂, CH₃ and/or CH₄ domains of an antibody polypeptide chain may be deleted, inserted or changed (see EPO 327 378 A1 to Morrison et al., the Trustees of Columbia University; U.S. Pat. No. 4,642,334 to Moore et al., DNAX; and U.S. Pat. No. 4,704,692 to Ladner et al., Genex). Once a final construct is obtained, the composite Hum4 V_(L), V_(H) antibodies may be produced in large quantities by injecting the host cell into the peritoneal cavity of pristane-primed mice, and after an appropriate time (about 1-2 weeks), harvesting ascites fluid from the mice, which yields a very high titer of homogeneous composite Hum4 V_(L), V_(H) antibodies, and isolating the composite Hum4 V_(L), V_(H) antibodies by methods well known in the art (see Stramignoni et al. (1983), Intl. J. Cancer, 31:543-552). The host cell are grown in vivo, as tumors in animals, the serum or ascites fluid of which can provide up to about 50 mg/mL of composite Hum4 V_(L), V_(H) antibodies. Usually, injection (preferably intraperitoneal) of about 10⁶ to 10⁷ histocompatible host cells into mice or rats will result in tumor formation after a few weeks. It is possible to obtain the composite Hum4 V_(L), V_(H) antibodies from a fermentation culture broth of procaryotic and eucaryotic cells, or from inclusion bodies of E. coli cells (see Buckholz and Gleeson (1991), BIO/TECHNOLOGY, 9:1067-1072. The composite Hum4 V_(L), V_(H) antibodies can then be collected and processed by well-known methods (see generally, Immunological Methods, vols. I & II, eds. Lefkovits, I. and Pernis, B., (1979 & 1981) Academic Press, New York, N.Y.; and Handbook of Experimental Immunology, ed. Weir, D., (1978) Blackwell Scientific Publications, St. Louis, Mo.).

The composite Hum4 V_(L), V_(H) antibodies can then be stored in various buffer solutions such as phosphate buffered saline (PBS), which gives a generally stable antibody solution for further use.

Uses

While it is possible for an antibody or fragment thereof to be administered alone—i.e. because they bear human C_(H) regions and will thus exert effector functions including complement mediated cytotoxicity and antibody dependent cell-mediated cytotoxicity—it is preferable to present it as a pharmaceutical formulation. The active ingredient may comprise, for topical administration, from 0.001% to 10% w/w, e.g., from 1% to 2% by weight of the formulation, although it may comprise as much as 10% w/w but preferably not in excess of 5% w/w and more preferably from 0.1% to 1% w/w of the formulation.

The topical formulations of the present invention, comprise an active ingredient together with one or more acceptable carrier(s) therefor and optionally any other therapeutic ingredients(s). The carrier(s) must be “acceptable” in the sense of being compatible with the other ingredients of the formulation and not deleterious to the recipient thereof.

Formulations suitable for topical administration include liquid or semi-liquid preparations suitable for penetration through the skin to the site of where treatment is required, such as liniments, lotions, creams, ointments or pastes, and drops suitable for administration to the eye, ear, or nose.

Drops according to the present invention may comprise sterile aqueous or oily solutions or suspensions and may be prepared by dissolving the active ingredient in a suitable aqueous solution of a bactericidal and/or fungicidal agent and/or any other suitable preservative, and preferably including a surface active agent. The resulting solution may then be clarified and sterilized by filtration and transferred to the container by an aseptic technique. Examples of bactericidal and fungicidal agents suitable for inclusion in the drops are phenylmercuric nitrate or acetate (0.002%), benzalkonium chloride (0.01%) and chlorhexidine acetate (0.01%). Suitable solvents for the preparation of an oily solution include glycerol, diluted alcohol and propylene glycol.

Lotions according to the present invention include those suitable for application to the skin or eye. An eye lotion may comprise a sterile aqueous solution optionally containing a bactericide and may be prepared by methods similar to those for the preparation of drops. Lotions or liniments for application to the skin may also include an agent to hasten drying and to cool the skin, such as an alcohol or acetone, and/or a moisturizer such as glycerol or an oil such as castor oil or arachis oil.

Creams, ointments or pastes according to the present invention are semi-solid formulations of the active ingredient for external application. They may be made by mixing the active ingredient in finely-divided or powdered form, alone or in solution or suspension in an aqueous or non-aqueous fluid, with the aid of suitable machinery, with a greasy or non-greasy basis. The basis may comprise hydrocarbons such as hard, soft or liquid paraffin, glycerol, beeswax, a metallic soap; a mucilage; an oil of natural origin such as almond, corn, arachis, castor or olive oil; wool fat or its derivatives, or a fatty acid such as stearic or oleic acid together with an alcohol such as propylene glycol or macrogels. The formulation may incorporate any suitable surface active agent such as an anionic, cationic or non-ionic surface active such as sorbitan esters or polyoxyethylene derivatives thereof. Suspending agents such as natural gums, cellulose derivatives or inorganic materials such as silicaceous silicas, and other ingredients such as lanolin, may also be included.

Kits according to the present invention include frozen or lyophilized humanized antibodies or humanized antibody fragments to be reconstituted, respectively, by thawing (optionally followed by further dilution) or by suspension in a (preferably buffered) liquid vehicle. The kits may also include buffer and/or excipient solutions (in liquid or frozen form)—or buffer and/or excipient powder preparations to be reconstituted with water—for the purpose of mixing with the humanized antibodies or humanized antibody fragments to produce a formulation suitable for administration. Thus, preferably the kits containing the humanized antibodies or humanized antibody fragments are frozen, lyophilized, pre-diluted, or pre-mixed at such a concentration that the addition of a predetermined amount of heat, of water, or of a solution provided in the kit will result in a formulation of sufficient concentration and pH as to be effective for in vivo or in vitro use in the treatment or diagnosis of cancer. Preferably, such a kit will also comprise instructions for reconstituting and using the humanized antibody or humanized antibody fragment composition to treat or detect cancer. The kit may also comprise two or more component parts for the reconstituted active composition. For example, a second component part—in addition to the humanized antibodies or humanized antibody fragments—may be bifunctional chelant, bifunctional chelate, or a therapeutic agent such as a radionuclide, which when mixed with the humanized antibodies or humanized antibody fragments forms a conjugated system therewith. The above-noted buffers, excipients, and other component parts can be sold separately or together with the kit.

It will be recognized by one of skill in the art that the optimal quantity and spacing of individual dosages of a humanized antibody or humanized antibody fragment of the invention will be determined by the nature and extent of the condition being treated, the form, route and site of administration, and the particular animal being treated, and that such optima can be determined by conventional techniques. It will also be appreciated by one of skill in the art that the optimal course of treatment, i.e., the number of doses of an antibody or fragment thereof of the invention given per day for a defined number of days, can be ascertained by those skilled in the art using conventional course of treatment determination tests.

The subject humanized antibodies may also be administered in combination with other anti-cancer agents, e.g., other antibodies or drugs. Also, the subject humanized antibodies or fragments may be directly or indirectly attached to effector moieties having therapeutic activity. Suitable effector moieties include by way of example cytokines (IL-2, TNF, interferons, colony stimulating factors, IL-1, etc.), cytotoxins (Pseudomonas exotoxin, ricin, abrin, etc.), radionuclides, such as ⁹⁰Y, ¹³¹I, ^(99m)Tc, ¹¹¹In, ¹²⁵I, among others, drugs (methotrexate, daunorubicin, doxorubicin, etc.), immunomodulators, therapeutic enzymes (e.g., beta-galactosidase), anti-proliferative agents, etc. The attachment of antibodies to desired effectors is well known.

See, e.g., U.S. Pat. No. 5,435,990 to Cheng et al. Moreover, bifunctional linkers for facilitating such attachment are well known and widely available. Also, chelators (chelants and chelates) providing for attachment of radionuclides are well known and available.

The composite Hum4 V_(L), V_(H) antibodies provide unique benefits for use in a variety of cancer treat-ments. In addition to the ability to bind specifically to malignant cells and to localize tumors and not bind to normal cells such as fibroblasts, endothelial cells, or epithelial cells in the major organs, the composite Hum4 V_(L), V_(H) antibodies may be used to greatly minimize or eliminate HAMA responses thereto. Moreover, TAG-72 contains a variety of epitopes and thus it may be desirable to administer several different composite Hum4 V_(L), V_(H) antibodies which utilize a variety of V_(H) in combination with Hum4 V_(L).

Specifically, the composite Hum4 V_(L), V_(H) antibodies are useful for, but not limited to, in vivo and in vitro uses in diagnostics, therapy, imaging and biosensors.

The composite Hum4 V_(L), V_(H) antibodies may be incorporated into a pharmaceutically acceptable, non-toxic, sterile carrier. Injectable compositions of the present invention may be either in suspension or solution form. In solution form the complex (or when desired the separate components) is dissolved in a pharmaceutically acceptable carrier. Such carriers comprise a suitable solvent, preservatives such as benzyl alcohol, if needed, and buffers. Useful solvents include, for example, water, aqueous alcohols, glycols, and phosphonate or carbonate esters. Such aqueous solutions generally contain no more than 50 percent of the organic solvent by volume.

Injectable suspensions require a liquid suspending medium, with or without adjuvants, as a carrier. The suspending medium can be, for example, aqueous polyvinyl-pyrrolidone, inert oils such as vegetable oils or highly refined mineral oils, or aqueous carboxymethylcellulose. Suitable physio-logically-acceptable adjuvants, if necessary to keep the complex in suspension, may be chosen from among thickeners such as carboxymethylcellulose, polyvinyl-pyrrolidone, gelatin and the alginates. Many surfactants are also useful as suspending agents, for example, lecithin, alkylphenol, polyethylene oxide adducts, naphthalenesulfonates, alkylbenzenesulfonates, and the polyoxyethylene sorbitan esters. Many substances which effect the hydrophobicity, density, and surface tension of the liquid suspension medium can assist in making injectable suspensions in individual cases. For example, silicone antifoams, sorbitol, and sugars are all useful suspending agents.

Methods of preparing and administering conjugates of the composite Hum4 V_(L), V_(H) antibody, and a therapeutic agent are well known or readily determined. Moreover, suitable dosages will depend on the age and weight of the patient and the therapeutic agent employed and are well known or readily determined.

Conjugates of a composite Hum4 V_(L), V_(H) antibody and an imaging marker may be administered in a pharma-ceutically effective amount for the in vivo diagnostic assays of human carcinomas, or metastases thereof, in a patient having a tumor that expresses TAG-72 and then detecting the presence of the imaging marker by appropriate detection means.

Administration and detection of the conjugates of the composite Hum4 V_(L), V_(H) antibody and an imaging marker, as well as methods of conjugating the composite Hum4 V_(L), V_(H) antibody to the imaging marker are accomplished by methods readily known or readily determined. The dosage of such conjugate will vary depending upon the age and weight of the patient. Generally, the dosage should be effective to visualize or detect tumor sites, distinct from normal tissues. Preferably, a one-time dosage will be between 0.1 mg to 200 mg of the conjugate of the composite Hum4 V_(L) anti-body and imaging marker per patient.

Examples of imaging markers which can be conjugated to the composite Hum4 V_(L), V_(H) antibody are well known and include substances which can be detected by diagnostic imaging using a gamma scanner or hand held gamma probe, and substances which can be detected by nuclear magnetic resonance imaging using a nuclear magnetic resonance spectrometer.

Suitable, but not limiting, examples of substances which can be detected using a gamma scanner include ¹²⁵I, ¹³¹I, ¹²³I, ¹¹¹In, ¹⁰⁵Rh, ¹⁵³Sm, ⁶⁷Cu, ⁶⁷Ga, ¹⁶⁶Ho, ¹⁷⁷Lu, ¹⁸⁶Re, ¹⁸⁸Re and ^(99m)Tc.

An example of a substance which can be detected using a nuclear magnetic resonance spectrometer is gadolinium.

Conjugates of a composite Hum4 V_(L), V_(H) anti-bodies and a therapeutic agent may be administered in a pharmaceutically effective amount for the in vivo treatment of human carcinomas, or metastases thereof, in a patient having a tumor that expresses TAG-72. A “pharmaceutically effective amount” of the composite Hum4 V_(L) antibody means the amount of said antibody (whether unconjugated, i.e., a naked antibody, or conjugated to a therapeutic agent) in the pharmaceutical composition should be sufficient to achieve effective binding to TAG-72.

Exemplary naked antibody therapy includes, for example, administering heterobifunctional composite Hum4 V_(L), V_(H) antibodies coupled or combined with another antibody so that the complex binds both to the carcinoma and effector cells, e.g., killer cells such as T cells, or monocytes. In this method, the composite Hum4 V_(L) antibody-therapeutic agent conjugate can be delivered to the carcinoma site thereby directly exposing the carcinoma tissue to the therapeutic agent. Alter-natively, naked antibody therapy is possible in which antibody dependent cellular cytoxicity or complement dependent cytotoxicity is mediated by the composite Hum4 V_(L) antibody.

Examples of the antibody-therapeutic agent conjugates which can be used in therapy include antibodies coupled to radionuclides, such as ¹³¹I, ⁹⁰Y, ¹⁰⁵Rh, ⁴⁷Sc, ⁶⁷Cu, ²¹²Bi, ²¹¹At, ⁶⁷Ga, ¹²⁵I, ¹⁸⁶Re, ¹⁸⁸Re, ¹⁷⁷Lu, ^(99m)Tc, ¹⁵³Sm, ¹²³I and ¹¹¹In; to drugs, such as methotrexate, adriamycin; to biological response modifiers, such as interferon and to toxins, such as ricin.

Methods of preparing and administering conjugates of the composite Hum4 V_(L), V_(H) antibodies and a therapeutic agent are well known or readily determined. The pharmaceutical composition may be administered in a single dosage or multiple dosage form. Moreover, suitable dosages will depend on the age and weight of the patient and the therapeutic agent employed and are well known or readily determined.

Composite Hum4 V_(L), V_(H) antibodies, and parti-cularly composite Hum4 V_(L), V_(H) single chain antibodies thereof, are particularly suitable for radioimmunoguided surgery (RIGS). In RIGS, an antibody labeled with an imaging marker is injected into a patient having a tumor that expresses TAG-72. The antibody localizes to the tumor and is detected by a hand-held gamma detecting probe (GDP). The tumor is then excised (see Martin et al. (1988), Amer. J. Surg., 156:386-392; and Martin et al. (1986), Hybridoma, 5:S97-S108). An exemplary GDP is the Neoprobe™ scanner, commercially available from Neoprobe Corporation, Columbus, Ohio. The relatively small size and human character of the composite Hum4 V_(L), V_(H) single chain antibodies will accelerate whole body clearance and thus reduce the waiting period after injection before surgery can be effectively initiated.

Administration and detection of the composite Hum4 V_(L), V_(H) antibody-imaging marker conjugate may be accomplished by methods well known or readily determined.

The dosage will vary depending upon the age and weight of the patient, but generally a one time dosage of 0.1 mg to 200 mg of the composite Hum4 V_(L) antibody-marker conjugate per patient is administered.

EXAMPLES

The following nonlimiting examples are merely for illustration of the construction and expression of composite Hum4 V_(L), V_(H) antibodies. All temperatures not otherwise indicated are Centigrade. All percents not otherwise indicated are by weight.

Example 1

CC49 and CC83 were isolated from their respective hybridomas using pNP9 as a probe (see FIGS. 5A-B). CC49 V_(H) was obtained from p49 g1-2.3 (see FIG. 6) and CC83 V_(H) was obtained from p83 g1-2.3 (see FIG. 7), following the procedures set forth in EPO 0 365 997.

DNA encoding an antibody light chain was isolated from a sample of blood from a human following the protocol of Madisen et. al. (1987), Am. J. Med. Genet., 27:379-390), with several modifications. Two 5 mL purple-cap Vacutainer tubes (containing EDTA as an anticoagulant) were filled with blood and stored at ambient temperature for 2 hours. The samples were transferred to two 4.5 mL centrifuge tubes. To each tube was added 22.5 mL of filter-sterilized erythrocycte lysate buffer (0.155 M NH₄Cl and 0.17 M Tris, pH 7.65, in a volume ratio of 9:1), and incubated at 37° C. for 6.5 minutes The tubes became dark red due to the lysed red blood cells. The samples were centrifuged at 90° C. for 10 minutes, using an SS-34 rotor and a Sorvall centrifuge at 5,300 revolutions per minute (rpm) (˜3,400×g). The resulting white cell pellets were resuspended in 25 mL of 0.15 M NaCl solution. The white blood cells were then centrifuged as before. The pellets were resuspended in 500 μL of 0.15 M NaCl and transferred to 1.5 mL microcentrifuge tubes. The cells were pelleted again for 3 minutes, this time in the microcentrifuge at 3,000 rpm. Very few red blood cells remained on the pellet. After the supernatants were decanted from the 2 microcentrifuge tubes, 0.6 mL high TE buffer (100 mM Tris, pH 8.0) was added. The tubes were hand-shaken for between 10 and 15 minutes. The resulting viscous solution was extracted with phenol, phenol-chloroform and finally with just chloroform as described in Sambrook et al., supra. To 3.9 mL of pooled extracted DNA solution were added 0.4 mL NaOAc (3 M, pH 5), and 10 mL 100 percent ethanol. A white stringy precipitate was recovered with a yellow pipette tip, transferred into a new Eppendorf tube, washed once with 70 percent ethanol, and finally washed with 100 percent ethanol. The DNA was dried in vacuo for 1 minute and dissolved in 0.75 mL deionized water. A 20 μL aliquot was diluted to 1.0 mL and the OD 260 nm value was measured and recorded. The concentration of DNA in the original solution was calculated to be 0.30 mg/mL.

Oligonucleotides (oligos) were synthesized using phosphoramidite chemistry on a 380A DNA synthesizer (Applied Biosystems, Foster, Calif.) starting on 0.2 μM solid support columns. Protecting groups on the final products were removed by heating in concentrated ammonia solution at 55° C. for 12 hours. Crude mixtures of oligonucleotides (approximately 12 OD 260 nm units) were applied to 16 percent polyacrylamide-urea gels and electrophoresed. DNA in the gels was visualized by short wave UV light. Bands were cut out and the DNA eluted by heating the gel pieces to 65° C. for 2 hours. Final purification was achieved by application of the eluted DNA solution onto C-18 Sep-Pac™ columns (Millipore) and elution of the bound oligonucleotide with a 60 percent methanol solution. The pure DNA was dissolved in deionized, distilled water (ddH₂O) and quantitated by measuring OD 260 nm.

A GeneAmp™ DNA amplification kit (Cetus Corp., Emeryville, Calif.) was used to clone the Hum4 V_(L) germline gene by the polymerase chain reaction (PCR), which was set up according to the manufacturer's directions. A thermal cycler was used for the denaturation (94° C.), annealing (45° C.) and elongation (72° C.) steps. Each of the three steps in a cycle was carried out for 4 minutes; there was a total of 30 cycles.

Upstream of the regulatory sequences in the Hum4 V_(L) germline gene, there is a unique Cla I restriction enzyme site. Therefore, the 5′ end oligonucleotide for the PCR, called HUMVL (+) (FIG. 8A), was designed to include this Cla I site.

FIG. 9 shows the human J4 (HJ4) amino acid and DNA sequences. The first two amino acids (Leu-Thr) complete the CDR3 region, the remainder make up the FR4 region. Glu is underlined in HJ4 because in CC49 J5 a somatic mutation had occured in this codon converting GAG (for Glu) to GTG (for Val). The (↓) indicates the slice site and the beginning of the intron between the J and Cκ exons. DNA sequences underlined in HJ4 represent parts of the sequence used for the 3′ end PCR oligo.

FIGS. 10A-E present the DNA and amino acid sequence of Hum4 V_(L) in human/chimeric CC49H and CC83H. Specifically, the figure shows the entire DNA sequence of the Hum4 V_(L) gene Cla I-Hind III segment in pRL1001, clone #2. A single base difference occured at position 3461 and is marked by an asterik (*). The corresponding amino acid sequences in the coding exons are shown. The site of the Leu-Pro mutation in clone #7 is boxed. An arrow (↑) indicates the site of the single base deletion in clone #11. The coding strand is underlined to designate the sites used for hybridization of complementary oligonucleotide primers. In order the primers occur from the 5′ end as follows: HUMLIN1(−); HUMLIN2(−);

HUMLCDR1(−) and Hind III Cκ(−) (not shown).

The 3′ end oligonucleotide, called HUMVL(−) (FIG. 8B), contained a unique Hind III site; sufficient mouse intron sequence past the splicing site to permit an effective splice donor function; a human J4 sequence contiguous with the 3′ end of the V_(L) exon of Hum4 V_(L) to complete the CDR3 and FR4 sequences of the V_(L) domain (see FIGS. 9 and 10A-E); nucleotides to encode a tyrosine residue at position 94 in CDR3; and 29 nucleotides close to the 3′ end of the V_(L) exon of Hum4 V_(L) (shown underlined in the oligonucleotide HUMVL(−) in FIG. 8B) to anneal with the human DNA target. In total, this 3′ end oligonucleotide for the PCR was 98 bases long with a non-annealing segment (a “wagging tail”) of 69 nucleotides. A schematic of the Hum4 V_(L) gene target and the oligonucleotides used for the PCR are shown in FIG. 11. A 5′ end oligo (HUMV_(L)(+)) and the 3′-end oligo (HUMV_(L)(−)) used to prime the elongation reactions for Taq polymerase and the target Hum4 V_(L) gene are shown.

A PCR reaction was set up with 1 μg of total human DNA in a reaction volume of 100 pL. Primers HUMVL(−) and HUMVL(+) were each present at an initial concentration of 100 pmol.

Prior to the addition of Taq polymerase (2.5 units/reaction) 100 μLs of mineral oil were used to overlay the samples. Control samples were set up as outlined below. The samples were heated to 95° C. for 3 minutes. When the PCR was complete, 20 μL samples were removed for analysis by agarose gel electrophoresis.

Based on the known size of the Hum4 V_(L) DNA fragment to be cloned, and the size of the oligonucleotides used to target the gene, a product of 1099 bp was expected. A band corresponding to this size was obtained in the reaction (shown in lane 7, FIG. 12).

To prepare a plasmid suitable for cloning and subsequently expressing the Hum4 V_(L) gene, the plasmid pSV2neo was obtained from ATCC and subsequently modified. pSV2neo was modified as set forth below (see FIGS. 13A-B).

The preparation of pSV2neo-101 was as follows. Ten micrograms of purified pSV2neo were digested with 40 units of Hind III at 37° C. for 1 hour. The linearized plasmid DNA was precipitated with ethanol, washed, dried and dissolved in 10 μL of water. Two microliters each of 10 mM dATP, dCTP, dGTP and dTTP were added, as well as 2 μL of 10×ligase buffer (Stratagene, La Jolla, Calif.). Five units (1 μL) of DNA polymerase I were added to make blunt the Hind III sticky ends. The reaction mixture was incubated at room temperature for 30 minutes. The enzyme was inactivated by heating the mixture to 65° C. for 15 minutes. The reaction mixture was then phenol extracted and ethanol precipitated into a pellet. The pellet was dissolved in 20 μL deionized, distilled water. A 2 μL aliquot (ca. 1 μg) was then added to a standard 20 μL ligation reaction, and incubated overnight at 4° C.

Competent E. coli DH1 cells (Invitrogen) were transformed with 1 μL and 10 μL aliquots of a ligation mix (Invitrogen, San Diego, Calif.) according to the manufacturer's directions. Ampicillin resistant colonies were obtained on LB plates containing 100 μg/mL ampicillin. Selected clones grown in 2.0 mL overnight cultures were prepared, samples of plasmid DNA were digested with Hind III and Bam HI separately, and a correct representative clone selected.

The resulting plasmid pSV2neo-101 was verified by size mapping and the lack of digestion with Hind III.

A sample of DNA (10 μg) from pSV2neo-101 mini-lysate was prepared by digesting with 50 units of Bam HI at 37° C. for 2 hours. The linearized plasmid was purified from a 4 percent polyacrylamide gel by electroelution. The DNA ends were made blunt by filling in the Bam HI site using dNTPs and Klenow fragment, as described earlier for the Hind III site of pSV2neo-101.

A polylinker segment containing multiple cloning sites was incorporated at the Bam HI site of pSV2neo-101 to create pSV2neo-102, as shown in FIG. 14. The arrow (←) indicates the direction of the Eco RI site in the vector. Note that the polylinker could be inserted in both orientations such that the Bam HI site on the left side could also be regenerated. The nucleotides used to fill-in the Bam HI site are shown in italics. The top synthetic oligo was called (CH(+) while the complimentary strand was CH(−). Equimolar amounts of two oligonucleotides, CH(+) and CH(−) (shown in FIG. 14) were annealed by heating for 3 minutes at 90° C. and cooling to 50° C. Annealed linker DNA and blunt ended pSV2neo-101 were added, in a 40:1 molar volume, to a standard 20 μL ligation reaction. E. coli DH1 (Invitrogen) was transformed with 0.5 μL and 5 μL aliquots of the ligation mixture (Invitrogen). Twelve ampicillin resistant colonies were selected for analysis of plasmid DNA to determine whether the linker had been incorporated.

A Hind III digest of mini-lysate plasmid DNA revealed linker incorporation in six of the clones. The plasmid DNA from several clones was sequenced, to determine the number of linker units that were blunt-end ligated to pSV2neo-101 as well as the relative orientation(s) with the linker. Clones for sequencing were selected on the basis of positive digestion with Hind III.

A Sequenase™ sequencing kit (United States Biochemical Corp, Cleveland, Ohio) was used to sequence the DNA. A primer, NEO102SEQ, was used for sequencing and is shown in FIG. 15. It is complementary to a sequence located upstream from the BamHI site in the vector. The Bam HI site where the polylinker was inserted in pSV2neo-101 is boxed. Between 3 μg and 5 μg of plasmid DNA isolated from E. coli mini-lysates were used for sequencing. The DNA was denatured and precipitated prior to annealing, as according to the manufacturer's instructions. Electrophoresis was carried out at 1500 volts; gels were dried prior to exposure to Kodak X-ray film. Data was processed using a DNASIS™ computer program (Hitachi).

From the DNA sequence data of 4 clones analyzed (see photograph of autoradiogram representing the sequence data of 2 of these clones-FIG. 16, reading the sequence (going up) is in the 5′ to 3′ direction of the (+) strand), compared to the expected sequence in FIG. 14, two clones having the desired orientation were obtained. In both cases a single 30-base linker unit was incorporated , but in opposite orientations. The panel A-sequence resulted in pSV2neo-120; and the panel B sequence resulted in pSV2neo-102. A representative clone was selected and designated pSV2neo-102.

A human Cκ gene was inserted into pSV2neo-102 to form pRL1000. The human Cκ DNA was contained in a 5.0 kb Hind III-Bam HI fragment (see Hieter et al. (1980), Cell, 22:197-207).

A 3 μg sample of DNA from a mini-lysate of pSV2neo-102 was digested with Bam HI and Hind III. The vector DNA was separated from the small Bam HI-Hind III linker fragment, generated in the reaction, by electro-phoresis on a 3.75 percent DNA polyacrylamide gel. The desired DNA fragment was recovered by electroelution. A pBR322 clone containing the 5.0 kb Hind III-Bam HI fragment of the human Cκ gene (see Hieter et al., supra) was designated phumCκ. The 5.0 kb Hind III-Bam HI fragment was ligated with pSV2neo-102 and introduced into E. coli DH1 (Invitrogen). Ampicillin resistant colonies were screened and a clone containing the human Cκ gene was designated pRL1000.

Finally, pRL1000 clones were screened by testing mini-lysate plasmid DNA from E. coli with Hind III and Bam HI. A clone producing a plasmid which gave 2 bands, one at 5.8 kb (representing the vector) and the other at 5.0 kb (representing the human Cκ insert) was selected. Further characterization of pRL1000 was achieved by sequencing downstream from the Hind III site in the intron region of the human Cκ insert. The oligonucleotide used to prime the sequencing reaction was NEO102SEQ (see FIG. 15). Two hundred and seventeen bases were determined (see FIG. 17). A new oligonucleotide corresponding to the (−) strand near the Hind III site (shown in FIG. 17) was synthesized so that clones, containing the Hum4 V_(L) gene that were cloned into the Cla I and Hind III sites in pRL1000 (see FIGS. 13A-B), could be sequenced.

A Cla I-Hind III DNA fragment containing Hum4 V_(L) obtained by PCR was cloned into the plasmid vector pRL1000. DNA of pRL1000 and the Hum4 V_(L) were treated with Cla I and Hind III and the fragments were gel purified by electrophoresis, as described earlier.

The pRL1000 DNA fragment and fragment containing Hum4 V_(L) gene were ligated, and the ligation mixture used to transform E. coli DH1 (Invitrogen), following the manufacturer's protocol. Ampicillin resistant clones were screened for the presence of the Hum4 V_(L) gene by restriction enzyme analysis and a representative clone designated pRL1001 (shown in FIG. 18). This is the expression vector to introduce the human anti-tumor L chain gene in Sp2/0 cells.

Four plasmids having the correct Cla I-Hind III restriction pattern were analyzed further by DNA sequencing of the insert region (see FIG. 19). Hind III Cκ(−) (shown by underlining on the plus strand to which it hybridizes in FIG. 17), HUMLIN1(−) (shown by underlining on the plus strand to which it hybridizes in FIGS. 10A-E), HUMLIN2(−) (shown by underlining on the plus strand to which it hybridizes in FIGS. 10A-E) and HUMLCDR1(−) (shown by underlining on the plus strand to which it hybridizesin FIG. 10) were used as the sequencing primers. Two out of the four plasmids analyzed had the expected sequence in the coding regions (FIG. 19, clones 2 and 9). The gel is read in the 5′ to 3′ direction on the (−) strand, from the Hind III Cκ(−) primer. Clones 2 and 9 were equivalent to the expected sequence, clone 7 had a single base base substitution (marked by *) and clone 11 had a single base deletion (marked by →).

Clone 2 was chosen and used for generating sufficient plasmid DNA for cell transformations and other analysis. This plasmid was used for sequencing through the Hum4 V_(L), and the upstream region to the Cla I site. Only one change at nucleotide position 83 from a C to a G (FIGS. 10A-E) was observed, compared to a published sequence (Klobeck et al. (1985), supra). The DNA sequence data also indicates that the oligonucleotides used for PCR had been correctly incorporated into the target sequence.

A Biorad Gene Pulser™ apparatus was used to transfect Sp2/0 cells with linearized plasmid DNAs containing the light or heavy chain constructs. The Hum4 V_(L) was introduced into Sp2/O cells along with corresponding heavy chains by the co-transfection scheme indicated in Table 1.

TABLE 1 Cell Line DNA Added Designation L Chain H Chain H Chain MP1-44H 20 μg 15 μg  0 μg MP1-84H 20 μg  0 μg 15 μg

A total of 8.0×10⁶ Sp2/0 cells were washed in sterile PBS buffer (0.8 mL at 1×10⁷ viable cells/mL) and held on ice for 10 minutes. DNA of pRL1001, linearized at the Cla I site, and DNA of either p49 g1-2.3 or p83 g1-2.3, linearized at their respective Nde I sites, were added, in sterile PBS, to the cells (see protocol—Table 2) and held at 0° C. for a further 10 minutes. A single 200 volt, 960 μF electrical pulse lasting between 20 and 30 milliseconds was used for the electroporation. After holding the perturbed cells on ice for 5 minutes, 25 mL of RPMI medium with 10 percent fetal calf serum were introduced, and 1.0 mL samples aliquoted in a 24 well tissue culture plate. The cells were incubated at 37° C. in a 5 percent CO₂ atmosphere. After 48 hours, the media was exchanged with fresh selection media, now containing both 1 mg/mL Geneticin (G418) (Difco) and 0.3 μg/ml mycophenolic acid/gpt medium. Resistant cells were cultured for between 7 and 10 days.

Supernatants from wells having drug resistant colonies were tested on ELISA plates for activity against TAG-72. A roughly 10 percent pure TAG-72 solution prepared from LSI74T tumor xenograft cells was diluted 1:40 and used to coat flexible polyvinyl chloride microtitration plates (Dynatech Laboratories, Inc.). Wells were air-dried overnight, and blocked the next day with 1 percent BSA. Supernatant samples to be tested for anti-TAG-72 antibody were added to the washed wells and incubated for between 1 and 2 hours at 37° C. Alkaline phosphatase labeled goat anti-human IgG (diluted 1:250) (Southern Biotech Associates, Birmingham, Ala.) was used as the probe antibody. Incubation was for 1 hour. The substrate used was p-nitrophenylphosphate. Color development was terminated by the addition of 1.0 N NaOH. The plates were read spectrophotometrically at 405 nm and 450 nm, and the values obtained were 405 nm-450 nm.

Those samples producing high values in the assay were subcloned from the original 24 well plate onto 96 well plates. Plating was done at a cell density of half a cell per well (nominally 50 cells) to get pure monoclonal cell lines. Antibody producing cell lines were frozen down in media containing 10 percent DMSO.

Two cell lines were procured having the designations: MP1-44H and MP1-84H. MP1-44H has the chimeric CC49 γ1 heavy chain with the Hum4 V_(L) light chain; and MP1-84H has the chimeric CC83 γ1 heavy-chain with the Hum4 V_(L) light chain. A 1.0 L spinner culture of the cell line of the cell line MP1-44H was grown at 37° C. for 5 days for antibody production. The culture supernatant was obtained free of cells by centrifugation and filtration through a 0.22 micron filter apparatus. The clarified supernatant was passed over a Protein A cartridge (Nygene, N.Y.). Immunoglobulin was eluted using 0.1 M sodium citrate buffer, pH 3.0. The pH of the eluting fractions containing the antibody was raised to neutrality by the addition of Tris base, pH 9.0. The antibody-containing fractions were concentrated and passed over a Pharmacia Superose 12 HR 10/30 gel filtration column. A protein was judged to be homogeneous by SDS polyacrylamide gel electrophoresis. Isoelectric focusing further demonstrated the purity of MP1-44H.

The biological performance of the human composite antibody, MP1-44H, was evaluated by comparing immunohistochemistry results with two other anti-TAG-72 antibodies CC49 (ATCC No. HB 9459) and Ch44 (ATCC No. HB 9884). Sections of human colorectal tumor embedded in paraffin were tested with the three antibodies by methods familiar to those skilled in this art. All three antibodies gave roughly equivalent binding recognition of the tumor antigen present on the tumor tissue sample.

A further test of the affinity and biological integrity of the human composite antibody MP1-44H was a competition assay, based on cross-competing radioiodine-labeled versions of the antibody with CC49 and Ch44 in all combinations. From the data shown in FIGS. 20A-C, it is apparent that the affinity of all 3 antibodies is equivalent and can bind effectively to tumor antigen.

MP1-44H (ATCC HB 10426) and M 1-84H (ATCC HB 10427) were deposited at the American Type Culture Collection (ATCC, P.O. Box 1549, Manassas, Va. 20108). The contract with ATCC provides for permanent availability of the cell lines to the public on the issuance of the U.S. patent describing and identifying the deposit or the publications or upon the laying open to the public of any U.S. or foreign patent application, whichever comes first, and for availability of the cell line to one determined by the U.S. Commissioner of Patents and Trademarks to be entitled thereto according to 35 CFR §122 and the Commissioner's rules pursuant thereto (including 37 CFR §1.14 with particular reference to 886 OG 638). The assignee of the present application has agreed that if the cell lines on deposit should die or be lost or destroyed when cultivated under suitable conditions for a period of thirty (30) years or five (5) years after the last request, it will be promptly replaced on notification with viable replacement cell lines.

Example 2

Single-chain antibodies consist of a V_(L), V_(H) and a peptide linker joining the V_(L) and V_(H) domains to produce SCFVs. A single chain antibody, SCFV1, was constructed to have the Hum4 V_(L) as V Domain 1 and CC49 V_(H) as V Domain 2 (see FIG. 21).

The polypeptide linker which joins the two V domains was encoded by DNA introduced at the 3′ end of the Hum4 V_(L) DNA during a PCR. The oligonucleotides SCFV1a and SCFV2 were designed to obtain the DNA segment incorporating part of the yeast invertase leader sequence, the Hum4 V_(L) and the SCFV linker.

The polypeptide linker for SCFV1 was encoded in oligonucleotide SCFV1b (see below). The underlined portions of the oligonucleotides SCFV1a and SCFV1b are complementary to sequences in the Hum4 V_(L) and linker respectively. The sequences of SCFV1 a and SCFV1b are as follows, with the hybridizing sequences underlined:

SCFV1a with the Hind III in bold:

5′-CTGCAAGCTTCCTTTTCCTTTTGGCTGGTTTTGCAGCCAAAATATCTGCAGACATCGTGATGACCCAGTC-3′ (SEQ ID NO:1)

SCFV1b with the Aat II site in bold:

5′-CGTAAGACGTCTAAGGAACGAAATTGGGCCAATT GTTCTGAGGAGACCGAACCTGACTCCTTCACCTTGGTCCCTCCGCCG-3′ (SEQ ID NO:2)

The target DNA in the PCR was pRL1001 (shown in FIG. 18). The PCR was performed pursuant to the teachings of Mullis et al., supra. A DNA fragment containing the Hum4 V_(L)-linker DNA component for the construction of SCFV1 was obtained and purified by polyacrylamide gel electrophoresis according to the teachings of Sambrook et al., supra.

p49 g1-2.3, containing CC49 V_(H), was the target DNA in the PCR. PCR was performed according to the methods of Mullis et al., supra. The oligonucleotides used for the PCR of CC49 V_(H) are as follows, with the hybridizing sequences underlined:

SCFV1c, with the Aat II site in bold:

5′-CTTAGACGTCCAGTTGCAGCAGTCTGACGC-3′ (SEQ ID NO:3)

SCFV1d, with the Hind III site in bold:

5′-GATCAAGCTTCACTAGGAGACGGTGACTGAGGTTCC-3′ (SEQ ID NO:4)

The purified Hum4 V_(L)-linker and V_(H) DNA fragments were treated with Aat II (New England Biolabs, Beverly, Mass.) according to the manufacturer's protocol, and purified from a 5 percent polyacrylamide gel after electrophoresis. An equimolar mixture of the Aat II fragments was ligated overnight. The T4 DNA ligase was heat inactivated by heating the ligation reaction mixture at 65° C. for 10 minutes. Sodium chloride was added to the mixture to give a final concentration of 50 mM and the mixture was further treated with Hind III. A Hind III DNA fragment was isolated and purified from a 4.5 percent polyacrylamide gel and cloned into a yeast expression vector (see Carter et al. (1987), In: DNA Cloning, A Practical Approach, Glover (ed.) Vol III: 141-161). The sequence of the fragment, containing the contiguous SCFV1 construct, is set forth in FIGS. 22A-C.

The anti-TAG-72 SCFV1 described herein utilized the yeast invertase leader sequence (shown as positions −19 to −1 of FIGS. 22A-C), the Hum4 V_(L) (shown as positions 1 to 113 of FIGS. 22A-C), an 18 amino acid linker (shown as positions 114 to 132 of FIGS. 22A-C) and CC49 V_(H) (shown as positions 133 to 248 of FIGS. 22A-C).

The complete DNA and amino acid sequence of SCFV1 is given in FIGS. 22A-C. The oligonucleotides used to sequence the SCFV1 are set forth below.

TPI:

5′-CAATTTTTTGTTTGTATTCTTTTC-3′ (SEQ ID NO:5).

HUVKF3:

5′-CCTGACCGATTCAGTGGCAG-3′ (SEQ ID NO:6).

DC113:

5′-TCCAATCCATTCCAGGCCCTGTTCAGG-3′ (SEQ ID NO:7).

SUC2T:

5′-CTTGAACAAAGTGATAAGTC-3′ (SEQ ID NO:8).

Example 3

A plasmid, pCGS517 (FIG. 23), containing a prorennin gene was digested with Hind III and a 6.5 kb fragment was isolated. The plasmid pCGS517 has a triosephosphate isomerase promoter, invertase [SUC2] signal sequence, the prorennin gene and a [SUC2] terminator. The Hind III-digested SCFV1 insert obtained above (see FIGS. 23A-B) was ligated overnight with the Hind III fragment of pCGS517 using T4 DNA ligase (Stratagene, La Jolla, Calif.).

The correct orientation existed when the Hind III site of the insert containing part of the invertase signal sequence ligated to the vector DNA to form a gene with a contiguous signal sequence. E. coli DHI (Invitrogen) cells were transformed and colonies screened using a filter-microwave technique (see Buluwela, et al. (1989), Nucleic Acids Research, 17:452). From a transformation plate having several hundred colonies, 3 positive clones were obtained. Digesting the candidate plasmids with Sal I and Kpn I, each a single cutter, differentiated between orientations by the size of the DNA fragments produced. A single clone, pDYSCFV1 (FIGS. 23A-B), had the correct orientation and was used for further experimentation and cloning. The probe used was derived from pRL1001, which had been digested with Kpn I and Cla I (see FIG. 18). The probe DNA was labeled with ³²P a-dCTP using a random oligonucleotide primer labeling kit (Pharmacia LKB Biotechnology, Piscataway, N.J.).

The next step was to introduce the Bgl II-Sal 1 fragment from pDYSCFV1 into the same restriction sites of another vector (ca. 9 kb), which was derived from pCGS515 (FIG. 23), to give an autonomously replicating plasmid in S. cerevisiae.

DNA from the vector and insert were digested in separate reactions with Bgl II and Sal I using 10×buffer number 3 (50 MM Tris-HCI (pH 8.0), 100 mM NaCl, BRL). The DNA fragment from pDYSCFV1 was run in and electroeluted from a 5 percent polyacrylamide gel and the insert DNA was run and electroeluted from a 3.75 percent polyacrylamide gel. A standard ligation using T4 DNA ligase (Stratagene, La Jolla, Calif.) and a transformation using E. coli DH1 (Invitrogen) was carried out. Out of 6 clones selected for screening with Bgl II and Sal I, all 6 were correctly oriented, and one was designated pCGS515/SCFV1 (FIGS. 23A-B).

DNA sequencing of pCGS515/SCFVI DNA was done using a Sequenase™ kit (U.S. Biochemical, Cleveland, Ohio) using pCGS515/SCFV1 DNA. The results have been presented in FIGS. 22A-C and confirm the sequence expected, based on the linker, the Hum4 V_(L) and the CC49 V_(H).

Transformation of yeast cells using the autonomosly replicating plasmid pCGS515/SCFV1 was carried out using the lithium acetate procedures described in Ito et al. (1983), J. Bacteriol., 153:163-168; and Treco (1987), In: Curent Protocols in Molecular Biology, Ausebel et al. (eds), 2:13.71-13.7.6. The recipient strain of S. cerevisiae was CGY1284 having the genotype—MAT a (mating strain a), ura 3-52 (uracil auxotrophy), SSC1-1 (supersecreting 1), and PEP4⁺ (peptidase 4 positive).

Transformed clones of CGY1284 carrying SCFV plasmids were selected by their ability to grow on minimal media in the absence of uracil. Transformed colonies appeared within 3 to 5 days. The colonies were transferred, grown and plated in YEPD medium. Shake flasks were used to provide culture supernatant with expressed product. An ELISA procedure was used to detect biological activity of the SCFV1. The assay was set up such that the SCFV would compete with biotinylated CC49 (biotin-CC49) for binding to the TAG-72 antigen on the ELISA plate.

SCFV1 protein was partially purified from a crude yeast culture supernatant, using a Superose 12 gel filtration column (Pharmacia LKB Biotechnology), and found to compete with biotinylated CC49 in the competition ELISA. These results demonstrate that the SCFV1 had TAG-72 binding activity.

The SCFV1 protein was detected by a standard Western protocol (see Towbin et al. (1979), Proc. Natl. Acad. Sci., USA, 76:4350-4354). The detecting agent was biotinylated FAID14 (ATCC No. CRL 10256), an anti-idiotypic monoclonal antibody prepared from mice that had been immunized with CC49. A band was visualized that had an apparent molecular weight of approximately 26,000 daltons, the expected size of SCFV1. This result demonstrated that the SCFV1 had been secreted and properly processed.

Example 4

The following example demonstrates the cloning of human V_(H) genes into a SCFV plasmid construct containing sequence coding for the Hum4 V_(L) and a 25 amino acid linker called UNIHOPE.

A vector was prepared from plasmid pRW 83 containing a chloramphenicol resistance (Cam^(r)) gene for clone selection, and a penP gene with a penP promoter and terminator (see Mezes, et al. (1983), J. Biol. Chem., 258:11211-11218) and the pel B signal sequence (see Lei, et al. (1987), supra) . The vector was designated Fragment A (see FIGS. 24A-B). The penP gene was removed with a Hind III/Sal I digest.

The penP promoter and pel B signal sequence were obtained by a PCR using pRW 83 as a template and oligonucleotides penP1 and penP2 as primers. The fragment was designated Fragment B (see FIGS. 24A-B). A Nco I enzyme restriction site was introduced at the 3′ end of the signal sequence region by the penP2 oligonucleotide.

penP1:

5′-CGATAAGCTTGAATTCCATCACTTCC-3′ (SEQ ID NO:9)

penP2:

5′-GGCCATGGCTGGTTGGGCAGCGAGTAATAACAATCCAGCG GCTGCCGTAGGCAATAGGTATTTCATCAAAATCGTCTCCCTCCGTTTGAA-3′ (SEQ ID NO:10)

A SCFV comprised of a Hum4 V_(L), a CC49 V_(H), and an 18 amino acid linker (Lys Glu Ser Gly Ser Val Ser Ser Glu Gln Leu Ala Gin Phe Arg Ser Leu Asp) was obtained from pCGS515/SCFV1 by PCR using oligonucleotides penP3 and penP6. This fragment was designated Fragment D (see FIGS. 24A-B). A Bcl I site was introduced at the 3′ end of the V_(H) region by the penP6 oligonucleotide.

penP3:

5′-GCTGCCCAACCAGCCATGGCCGACATCGTGATGACCCAGTCTCC-3′ (SEQ ID NO:11)

penP6(−):

5′-CTCTTGATCACCAAGTGACTTTATGTAAGATGATGTTTTG ACGGATTCATCGCAATGTTTTTATTTGCCGGAGACGGTGACTGAGGTTCC-3′ (SEQ ID NO:12)

Fragments B and D were joined by PCR using oligonucleotides penP1 and penP6, following the procedures of Horton et al., supra. The new fragment was designated E (see FIGS. 24A-B).

Fragment C containing the penP termination codon was isolated by digesting pRW 83 with Bcl I and Sal I, and designated Fragment C. pRW 83 was isolated from E. coli strain GM161, which is DNA methylase minus or dam⁻. Plasmid pSCFV 31 (see FIGS. 24A-B) was created with a three part ligation Fragments A, C, and E.

The Nco I restriction enzyme site within the Cam^(r) gene and the Hind III site located at the 5′ end of the penP promoter in pSCFV 31 were destroyed through a PCR DNA amplification using oligonucleotides Nco1.1 and Nco1.3(−) to generate an Eco RI-Nco I fragment and oligonucleotides Nco1.2 and Nco1.4c(−) to generate a Nco I to Eco RI fragment. These two fragments were joined by PCR-SOE using oligonucleotides Nco1.1 and Nco1.4c(−). The oligonucleotides are set forth below:

Nco1.1:

5′-TCCGGAATTCCGTATGGCAATGA-3′ (SEQ ID NO:13)

Nco1.3(−):

5′-CTTGCGTATAATATTTGCCCATCGTGAAAACGGGGGC-3′ (SEQ ID NO:14)

Nco1.2:

5′-ATGGGCAAATATTATACGCAAG-3′ (SEQ ID NO:15)

Nco1.4c(−):

5′-CACTGAATTCATCGATGATAAGCTGTCAAACATGAG-3′ (SEQ ID NO:16)

pSCFV 31 was digested with Eco RI and the larger fragment was isolated by polyacrylamide gel electrophoresis. To prevent self ligation, the DNA was dephosphorylated using calf intestinal alkaline phosphatase according to the teachings of Sambrook et al., supra.

A two part ligation of the larger pSCFV 31 digested fragment and the PCR-SOE fragment, described above, resulted in the creation of pSCFV 31b (see FIGS. 25A-B).

pSCFV 31b was digested with Nco I and Sal I and a fragment containing the Cam^(r) gene was isolated.

The Hum4 V_(L) was obtained by PCR DNA amplification using pCGS515/SCFV1 as a template and oligonucleotides 104BH1 and 104BH2(−) as primers.

104BH1:

5′-CAGCCATGGCCGACATCGTGATGACCCAGTCTCCA-3′ (SEQ ID NO:17)

104BH2(−):

5′-AAGCTTGCCCCATGCTGCTTTAACGTTAGTTTTATCTGCTGGAGACAGAGTGCCTTCTGCCTCCACCTTGGTCCCTCCGCCGAAAG-3′ (SEQ ID NO:18)

The CC49 V_(H) was obtained by PCR using p49 g1-2.3 (FIG. 6) as a template and oligonucleotides 104B3 and 104B4(−) as primers. A Nhe I enzyme restriction site was introduced just past the termination codon in the 3′ end (before the Bcl I site) by oligonucleotide 104B4(−).

104B3:

5′-GTTAAAGCAGCATGGGGCAAGCTTATGACTCAGTTGCAGCAGTCTGACGC-3′ (SEQ ID NO:19)

104B4(−):

5′-CTCTTGATCACCAAGTGACTTTATGTAAGATGATGTTTTGACGGATTCATCGCTAGCTTTTTATTTGCCATAATAAGGGGAGACGGTGACTGAGGTTCC-3′ (SEQ ID NO:20)

In the PCR which joined these two fragments using oligonucleotides 104BH1 and 104B4(−) as primers, a coding region for a 22 amino acid linker was formed.

A fragment C (same as above) containing the penP termination codon was isolated from pRW 83 digested with Bcl I and Sal I.

Plasmid pSCFV 33H (see FIGS. 25A-B) was created with a three part ligation of the vector, fragment C, and the SCFV fragment described above.

pSCFV 33H was digested with NcoI and NheI, and the DNA fragment containing the Cam^(r) gene was isolated as a vector. Hum4 V_(L) was obtained by PCR DNA amplification using pRL1001 (see FIG. 18) as a template and oligonucleotides UNIH1 and UNIH2(−) as primers. Oligonucleotides for the PCR were: UNIH1:

5′-CAGCCATGGCCGACATTGTGATGTCACAGTCTCC-3′ (SEQ ID NO:21)

The Nco I site is in bold and the hybridizing sequence is underlined.

UNIH2(−): 5′-GAGGTCCGTAAGATCTGCCTCGCTACCTAGCAAAAGGTCCTCAAGCTTGATCACCACCTTGGTCCCTCCGC-3′ (SEQ ID NO:22)

The Hind III site is in bold.

The CC49 V_(H) was obtained by a PCR using p49 g1 -2.3 (see FIG. 6) as a template and oligonucleotides UNI3 and UNI4(−) as primers.

UNI3:

5′-AGCGAGGCAGATCTTACGGACCTCGAGGTTCAGTTGCAGCAGTCTGAC-3′(SEQ ID NO:23).

The Xho I site is in bold and the hybridizing sequence is underlined.

UNI4(−):

5′-CATCGCTAGCTTTTTATGAGGAGACGGTGACTGAGGTTCC-3′ (SEQ ID NO:24).

The Nhe I site is in bold and the hybridizing sequence is underlined.

Oligonucleotides UNIH1 and UNI4(−) were used in the PCR-SOE amplification which joined the Hum4 V_(L) and CC49 V_(H) fragments and formed a coding region for a negatively charged fifteen amino acid linker. The DNA was digested with Nhe I and Nco I and ligated with the vector fragment from the Nco I-Nhe I digest of pSCFV 33H. The resultant plasmid was designated pSCFV UNIH (shown in FIGS. 25A-B).

With the construction of pSCFV UNIH, a universal vector for any SCFV was created with all the desired restriction enzyme sites in place.

pSCFV UNIH was digested with Hind III/Xho I, and the large DNA fragment containing the Cam^(r) gene, Hum4 V_(L) and CC49 V_(H) was isolated.

A fragment coding for a 25 amino acid linker, was made by annealing the two oligonucleotides shown below. The linker UNIHOPE is based on 205C SCAR™ linker (see Whitlow, (1990) Antibody Engineering: New Technology and Application Implications, IBC USA Conferences Inc, Mass.), but the first amino acid was changed from serine to leucine and the twenty-fifth amino acid were was changed from glycine to leucine, to accomodate the Hind III and Xho I restriction sites. The nucleotide sequence of the single chain portion of pSCFV Unihope H is shown in FIGS. 26A-D. Structural sequences are indicated by the amino acid sequence written above the DNA sequence. The symbols ← and → indicate the beginning and end of a given segment. The amino acid sequence of the linker is boxed.

The nucleotide sequence encoding the linker UNIHOPE is set forth below:

UNIHOPE (FIGS. 26A-D):

5′-TATAAAGCTTAGTGCGGACGATGCGAAAAAGGATGCTGCGAAGAAGGATGACGCTAAGAAAGACGATGCTAAAAAGGACCTCGAGTCTA-3′ (SEQ ID NO:25)

UNIHOPE(−) (FIGS. 26A-D):

5′-TAGACTCGAGGTCCTTTTTAGCATCGTCTTTCTTAGCGTCATCCTTCTTCGCAGCATCCTTTTTCGCATCGTCCGCACTAAGCTTTATA-3′ (SEQ ID NO:26)

The resulting strand was digested with Hind III/Xho I and ligated into the vector, thus generating the plasmid pSCFV UHH (shown in FIG. 27). Plasmid pSCFV UHH expresses a biologically active, TAG-72 binding SCFV consisting of the Hum4 V_(L) and CC49 V_(H) . The expression plasmid utilizes the β-lactamase penP promoter, pectate lyase pelB signal sequence and the penP terminator region. Different immunoglobulin light chain variable regions can be inserted in the Nco I-Hind III restriction sites, different SCFV linkers can be inserted in the Hind III-Xho I sites and different immunoglobulin heavy chain variable regions can be inserted in the Xho I-Nhe I sites.

E. coli AG1 (Stratagene) was transformed with the ligation mix, and after screening, a single chloramphenicol resistant clone, having DNA with the correct restriction map, was used for further work.

The DNA sequence and deduced amino acid sequence of the SCFV gene in the resulting plasmid are shown in FIG. 26.

E. coli AG1 containing pSCFV UHH were grown in 2 ml of LB broth with 20 μg/mL chloramphenicol (CAM 20). The culture was sonicated and assayed using a competition ELISA. The cells were found to produce anti-TAG-72 binding material. The competition assay was set up as follows: a 96 well plate was derivatized with a TAG-72 preparation from LS174T cells. The plate was blocked with 1% BSA in PBS for 1 hour at 31° C. and then washed 3 times. Twenty-five microliters of biotin CC49 (1/20,000 dilution of a 1 mg/mL solution) were added to the wells along with 25 μL of sample to be tested (competition step) and the plate was incubated for 30 minutes at 31° C. The relative amounts of TAG-72 bound to the plate, biotinylated CC49, streptavidin-alkaline phosphatase, and color development times were determined empirically in order not to have excess of either antigen or biotinylated CC49, yet have enough signal to detect competition by SCFV. Positive controls were cc49 at 5 μg/mL and CC49 Fab at 10 μL/mL. Negative controls were 1% BSA in PBS and/or concentrated LB. At the end of the competition step, unbound proteins were washed away.

Fifty microliters of a 1:1000 dilution of streptavidin conjugated with alkaline phosphatase (Southern Biotechnology Associates, Inc., Birmingham, Ala.) were added and the plate was incubated for 30 minutes at 31° C. The plate was washed 3 more times. Fifty microliters of a para-nitrophenylphosphate solution (Kirkegaard & Perry Laboratories, Inc., Gaithersburg, Md.) were added and the color reaction was allowed to develop for a minimum of 20 minutes. The relative amount of SCFV binding was measured by optical density scanning at 405-450 nm 10 using a microplate reader (Molecular Devices Corporation, Menlo Park, Calif.). Binding of the SCFV resulted in decreased binding of the biotinylated CC49 with a concomitant decrease in color development. The average value for triplicate test samples is shown in the table below:

Sample (50 μL) OD 405 nm - OD 450 nm (mixed 1:1 with CC49 Value Biotin) at 50 minutes Sonicate E. coli AG1/pSCFVUHH 0.072 clone 10 Sonicate E. coli AG1/pSCFVUHH 0.085 clone 11 CC49 at 5 mg/mL 0.076 CC49 Fab at 10 mg/mL 0.078 LB (negative control) 0.359

The data indicates that there was anti-TAG-72 activity present in the E. coli AGI/pSCFVUHH clone sonicate.

Example 5

The plasmid pSCFVUHH may be used to host other V_(H) genes on Xho I-Nhe I fragments and test in a SCFV format, following the procedures set forth below. A schematic for this process is shown in FIGS. 31A-B.

Isolating Total RNA from Peripheral Blood Lymphocytes

Blood from a normal, healthy donor is drawn into three 5 mL purple-cap Vacutainer tubes. Seven mL of blood are added to two 15 mL polypropylene tubes. An equal volume of lymphoprep (cat# AN5501, Accurate) is added and the solution is mixed by inversion. Both tubes are centrifuged at 1000 rpm and 18° C. for 20 minutes. The resulting white area near the top of the liquid (area not containing red blood cells) is removed from each sample and placed into two sterile polypropylene centrifuge tube. Ten mL of sterile PBS are added and the tube mixed by inversion. The samples are centrifuged at 1500 rpm and 18° C. for 20 minutes Total RNA is isolated from resulting pellet according to the RNAzol B Method (Chomczynski and Sacchi (1987), Analytical Biochemistry, 162:156-159). Briefly, the cell pellets are lysed in 0.4 mL RNAzol solution (cat#:CS-105, Cinna/Biotecx). RNA is solubilized by passing the cell pellet through a 1 mL pipet tip. Sixty μL of chloroform are added and the solution is shaken for 15 seconds. RNA solutions are then placed on ice for 5 minutes. Phases are separated by centrifugation at 12000×g and 4° C. for 15 minutes. The upper (aqueous) phases 30 are transferred to fresh RNase-free microcentrifuge tubes. One volume of isopropanol is added and the samples placed at −20° C. for 1 hour. The samples are then placed on dry ice for 5 minutes and finally centrifuged for 40 seconds at 14,000×g and 4° C. The resulting supernatant is removed from each sample and the pellet is dissolved in 144 μL of sterile RNase-free water. Final molarity is brought to 0.2 in NaCl. The DNA is reprecipitated by adding 2 volumes of 100% ethanol, leaving on dry ice for 10 minutes, and centrifugation at 14,000 rpm and 4° C. for 15 minutes. The supernatants are then removed, the pellets washed with 75% ethanol and centrifuged for 8 minutes at 12000×g and 4° C. The ethanol is then removed and the pellets dried under vacuum. The resulting RNA is then dissolved in 20 sterile water containing 1 μl RNasin (cat#:N2511, Promega).

cDNA Synthesis

cDNA synthesis is performed using a Gene Amp™ PCR kit (cat#:N808-0017 Perkin Elmer Cetus), RNasin™ (cat#:N2511, Promega), and AMV reverse transcriptase (cat#: M9004, Promega). The following protocol is used for each sample:

Components Amount MgCl₂ solution 4 μl 10 × PCR 2 μl buffer II dATP 2 μl dCTP 2 μl dGTP 2 μl dTTP 2 μl 3′ primer 1 μl (random hexamers) RNA sample 2 μl RNasin 1 μl AMV RT 1.5 μl

Samples are heated at 80° C. for 3 minutes then slowly cooled to 48° C. The samples are then centrifuged for 10 seconds. AMV reverse transcriptase is added to the samples which are then incubated for 30 minutes at 37° C. After incubation, 0.5 μl of each dNTP and 0.75 reverse transcriptase (cat#:109118, Boehringer Mannheim) are added. The samples are incubated for an additional 15 minutes at 37° C.

PCR Reaction

Oligonucleotides are designed to amplify human V_(H) genes by polymerase chain reaction. The 5′ oligonucleotides are set forth below:

HVH135.

5′-TATTCTCGAGGTGCA(AG)CTG(CG)TG(CG)AGTCTGG-3′ (SEQ ID NO:27)

HVH2A:

5′-TATTCTCGAGGTCAA(CG)TT(AG)A(AG)GGAGTCTGG-3′ (SEQ ID NO:28)

HVH46:

5′-TATTCTCGAGGTACAGCT(AG)CAG(CG)(AT)GTC(ACG)GG-3′(SEQ ID NO: 29)

The 3′oligonucleotides are set forth below:

JH1245:

5′-TTATGCTAGCTGAGGAGAC(AG)GTGACCAGGG-3′ (SEQ ID NO:30)

JH3:

5′-TTATGCTAGCTGAAGAGACGGTGACCATTG (SEQ ID NO:31)

JH6:

5′-TTATGCTAGCTGAGGAGACGGTGACCGTGG-3′ (SEQ ID NO:32)

PCR reactions are performed with a GeneAmp™ PCR kit (cat#:N808-0017, Perkin Elmer Cetus). Components are listed below:

Components Amount ddH₂O 75 μl 10 × buffer 10 μl dATP 2 μl dCTP 2 μl dGTP 2 μl dTTP 2 μl 1* Target DNA 1 μl 2* 5′ primer 2.5 μl 3′ primer 2.0 μl 3* AmpliTaq ™ Polymerase 1.3 μl

SUBSTANCE AMOUNT DNA 20 μl NEB Buffer #2 4.5 μl Nhe I 2 μl Xho I 2 μl ddH₂O 16.5 μl

Samples are incubated at 37° C. for one hour. After this incubation, an additional 1.5 μL Nhe I is added and samples are incubated an additional two hours at 37° C.

Purification of DNA

After the restriction enzyme digest, DNA is run on a 5 percent polyacrylamide gel (Sambrook et al. (1989), supra). Bands of 390-420 bp in size are excised from the gel. DNA is electroeluted and ethanol precipitated according to standard procedures.

PCR products resulting from oligonucleotide combinations are pooled together: JH1245 with HVH135, HVH2A and HVH46; JH3 with HVH135, HVH2A and HVH46; JH6 with HVH135, HVH2A and HVH46. The volume of the resulting pools are reduced under vacuum to 50 microliters. The pools are then purified from a 4 percent polyacrylamide gel (Sambrook et al. (1989), supra) to isolate DNA fragments. Bands resulting at 390-420 bp are excised from the gel. The DNA from excised gel slices is electroeluted according to standard protocols set forth in Sambrook, supra.

Isolation of pSCFVUHH Xho I/Nhe I Vector Fragment

Approximately 5 μg in 15 μL of pSCFVUHH plasmid is isolated using the Magic Mini-prep™ system (Promega). To this is added 5.4 μL OF 10×Buffer #2 (New England Biolabs), 45 units of Xho I (New England Biolabs), 15 units of Nhe I and 24 μL of ddH₂O. The reaction is allowed to proceed for 1 hour at 37° C. The sample is loaded on a 4% polyacrylamide gel, electrophoresed and purified by electroelution, as described earlier. The DNA pellet is dissolved in 20 μL of ddH₂O.

One hundred nanograms of pSCFVUHH digested with Xho I/Nhe I is ligated with a 1:1 molar ratio of purified human V_(H) inserts digested with Xho I and Nhe I using T4 DNA ligase (Stratagene). Aliquots are used to transform competent E. coli AG1 cells (Stratagene) according to the supplier's instructions.

GVWP hydrophilic membranes (cat# GVWP14250, Millipore) are placed on CAM 20 LB agar plates (Sambrook et al., 1989). One membrane is added to each plate. Four hundred microliters of the E. coli AG1 transformation suspension from above are evenly spread over the surface of each membrane. The plates are incubated for 16 hours at 37° C.

Preparation of TAG-72-Coated Membranes

A 1% dilution of partially purified tumor associated glycoprotein-72 (TAG-72) produced in LS174 T-cells is prepared in TBS (cat# 28376, Pierce). Ten milliliters of the TAG dilution are placed in a petri plate (cat# 8-757-14, Fisher) for future use. Immobilon-P PVDF transfer membranes (cat#SE151103, Millipore) are immersed in methanol. The membranes are then rinsed three times in sterile double distilled water. After the final wash, the excess water is allowed to drain. Each of the membranes are placed in 10 milliliters of dilute TAG-72. The membranes are incubated at ambient temperature from 1 hour with gentle shaking. After incubation, the membranes are blocked with Western blocking solution (25 mM Tris, 0.15 M NaCl, pH 7.6; 1% BSA) for about 1 hour at ambient temperature.

Blocking solution is drained from the TAG membranes. With the side exposed to TAG-72 facing up, the membranes are placed onto fresh CAM 20 plates. Resulting air pockets are removed. The bacterial membranes are then added, colony side up, to a TAG membrane. The agar plates are incubated for 24 to 96 hours at ambient temperatures.

The orientation of the TAG-72 and bacterial membranes are marked with permanent ink. Both membranes are removed from the agar surface. The TAG-72 membrane is placed in 20 ml of Western antibody buffer (TBS in 0.05% Tween-20, cat# P-1379, Sigma Chemical Co.; 1% BSA, cat#3203, Biocell Laboratories) containing 0.2 ng of CC49-Biotin probe antibody. The bacterial membranes are replaced on the agar surface in their original orientation and set aside. CC49-Biotin is allowed to bind to the TAG membranes for 1 hour at 31° C. with gentle shaking. The membranes are then washed three times with TTBS (TBS, 0.05% Tween-20) for 5 minutes on an orbital shaker at 300 rpm. Streptavidin alkaline phosphatase (cat# 7100-04, Southern Biotechnology Associates) is added to Western antibody buffer to produce a 0.1% solution. The TAG-72 membranes are each immersed in 16 milliliters of the streptavidin solution and allowed to incubate for 30 minutes at 31° C. with gentle shaking. After incubation, the membranes are washed as previously described. A final wash is then performed using Western alkaline phosphate buffer (8.4 g NaCO₃, 0.203 g MgCl₂—H₂O, pH 9.8), for 2 minutes at 200 rpm at ambient temperature. To develop the membranes, Western blue stabilized substrate (cat# S384B, Promega) is added to each membrane surface. After 30 minutes at ambient temperatures, development of the membranes is stopped by rinsing the membranes three times with ddH₂O. The membranes are then photographed and clear zones are corelated with colonies on the hydrophilic membrane, set aside earlier. Colony(ies) are isolated for growth in culture and used to prepare plasmid DNA for sequencing characterization. Also, the protein product is isolated to evaluate specificity and affinity.

Identification of Hum4 V_(L), Human V_(H) Combinations Using pATDFLAG

In a second assay system, Hum4 V_(L)—human V_(H) combinations are discovered that bind to TAG-72 according to the schematic, supra, except for the following a different plasmid vector, pATDFLAG, was used (see below): at the assay step, IBI MII antibody is used as a probe to detect any Hum4 V_(L)-V_(H) SCFV combinations that have bound to the hydrophobic membrane coated with TAG-72 and a sheep anti-mouse Ig antibody conjugated to horseradish peroxidase (Amersham, Arlington Heights, Ill.) is used to detect any binding of the MII antibody to TAG-72.

The plasmid pATDFLAG was generated from pSCFVUHH (see FIG. 29) to incorporate a Flag-coding sequence 3′ of any human V_(H) genes to be expressed contiguously with Hum4 V_(L). The plasmid pATDFLAG, when digested with Xho I and Nhe I and purified becomes the human V_(H) discovery plasmid containing Hum4 V_(L) in this SCFV format. The plasmid pATDFLAG was generated as follows. Plasmid pSCFVUHH treated with Xho I and Nhe I (isolated and described above) was used in a ligation reaction with the annealed FLAG and FLAGNC oligonucleotides.

FLAGC:

5′-TCGAGACAATGTCGCTAGCGACTACAAGGACGATGATGACAAATAAAAAC-3′ (SEQ ID NO:33)

FLAGNC:

5′-CTAGGTTTTTATTTGTCATCATCGTCCTTGTAGTCGCTAGCGACATTGTC-3′ (SEQ ID NO:34)

Equimolar amounts (1×10⁻¹⁰ moles of each of the oligonucleotides FLAGC and FLAGNC were mixed together using a ligation buffer (Stratagene). The sample is heated to 94° C. and is allowed to cool to below 35° C. before use in the ligation reaction below.

Ligation Reaction to Obtain pATDFLAG COMPONENT AMOUNT pSCFVUHH Xho I/Nhe I 1.5 μl vector ANNEALED 0.85 μl FLAGC/FLAGNC 10 × Ligation buffer 2 μl T4 DNA LIGASE 1 μl 10 MM ATP 2 μl ddH₂O 12.65 μl

This ligation reaction is carried out using the following components and amounts according the ligation protocol disclosed above. E. coli AG1 cells (Stratagene) are transformed with 3 μl of the above ligation reaction and colonies selected using CAM 20 plates. Clones having appropriate Nhe I, Xho I and Nhe I/Xho I restriction patterns are selected for DNA sequencing.

The oligonucleotide used to verify the sequence of the FLAG linker in pATDFLAG (see FIGS. 28A-C) is called PENPTSEQ: 5′-CTTTATGTAAGATGATGTTTTG-3′ (SEQ ID NO:35). This oligonucleotide is derived from the non-coding strand of the penP terminator region. DNA sequencing is performed using Sequenase™ sequencing kit (U.S. Biochemical, Cleveland, Ohio) following the manufacturer's directions. The DNA and deduced amino acid sequences of the Hum4 V_(L)-UNIHOPE linker-FLAG peptide of pATDFLAG is shown in FIGS. 28A-C.

Generation of pSC49FLAG

The CC49V_(H) is inserted into the sites of Xho I-Nhe I pATDFLAG (see FIG. 29) and evaluated for biological activity with the purpose of serving as a positive control for the FLAG assay system to detect binding to TAG-72. The new plasmid, called pSC49FLAG (see FIG. 29) is generated as follows. The plasmid pATDFLAG (5 mg, purified from a 2.5 ml culture by the Magic Miniprep™ system (Promega) is treated with Xho I and Nhe I and the large vector fragment purified as described above for pSCFVUHH. The CC49 V_(H) insert DNA fragment is obtained by PCR amplification from pSCFVUHH and oligonucleotides UNI3 as the 5′ end oligonucleotide and SC49FLAG as the 3′ end oligonucleotide. The resulting DNA and amino acid sequences of this SCFV antibody, with the FLAG peptide at the C-terminus, is shown in FIGS. 30A-D. The PCR reaction is carried out using 100 pmol each of the oligonucleotides, 0.1 ng of pSCFVUHH target DNA (uncut) and the standard protocol and reagents provided by Perkin Elmer Cetus. The DNA is first gel purfied, then treated with Xho I and Nhe I to generate sticky ends and purified from a 4% polyacrylamide gel and electroeluted as described earlier. The DNA vector (pATDFLAG treated with Xho I and Nhe I) and the insert (CC49 V_(H) PCR product from pSCFVUHH treated with Xho I and Nhe I) are ligated in a 1:1 molar ratio, using 100 ng vector DNA (Stratagene kit) and used to transform E. coli AG1 competent cells (Stratagene) according to the manufacturer's directions. A colony with the correct plasmid DNA is picked as the pSC49FLAG clone.

Ligation of pATDFLAG Vector with PCR Amplified Human V_(H) Inserts

The protocol for the ligation reaction is as follows:

COMPONENT AMOUNT DNA vector: pATDFLAG Xho I/Nhe 2.5 μL I Hum V_(H) (X) DNA inserts: Xho 6 μL I/Nhe I 10 mM ATP (Stratagene) 2 μL 10 × buffer (Stratagene) 2 μL T4 DNA ligase (Stratagene) 1 μL ddH₂O 6.5 μL

DNA vector, ATP, 10×buffer and ddH₂O are combined. DNA insert and T4 DNA ligase are then added. Ligation reactions are placed in a 4 L beaker containing H₂O at 18° C. The temperature of the water is gradually reduced by refrigeration at 4° C. overnight. This ligation reaction generates pHum4 V_(L)-hum V_(H) (X) (See FIG. 29).

Transformation of E. coli AG1 with pHum4 V_(L)-Hum V_(H) (X) Ligation Mix

Transformation of pATDFLAG into competent E. coli AG1 cells (Stratagene) is achieved following the supplier's protocol.

IBI MII Anti-FLAG Antibody Plate Assay

The first three steps, preparation of TAG-coated membranes, plating of bacterial membranes, and assembly of TAG and bacterial membranes, are the same as those described in the CC49-Biotin Competition Plate Assay.

After the 24 hour incubation at ambient temperatures, the membranes are washed with TTBS three times at 250 rpm for four minutes. The MII antibody (cat# IB13010, International Biotechnologies, Inc.) is then diluted with TBS to a concentration ranging from 10.85 μg/ml to 0.03 μg/ml. Ten millilters of the diluted antibody are added to each membrane. The membranes are then incubated for 1 hour at ambient temperatures and shaken on a rotary shaker at 70 rpm. After incubation, the MII antibody is removed and the membranes are washed three times at 250 rpm and ambient temperatures for 5 minutes. The final wash is removed and 20 milliters of a 1:2000 dilution of sheep anti-mouse horseradish peroxidase linked whole antibody (cat# NA931, Amersham) is prepared with TBS and added to each membrane. The membranes are again incubated for 1 hour at ambient temperatures and 70 rpm. Following incubation, the membranes are washed three times at 250 rpm and ambient temperature for 5 minutes each. Enzygraphic Webs (cat# IB8217051, International Biotechnologies, Inc.) are used to develop the membranes, according to the manufacturer's instructions. The membranes are then photographed.

Instead of seeing a clear zone on the developed membrane for a positive Hum4 V_(L)-V_(H) (X) clone producing an SCFV that binds to TAG-72, (as seen with the competition screening assay) in this direct FLAG-detecting assay, a blue-purple spot is indicative of a colony producing a SCFV that has bound to the TAG-72 coated membrane. The advantage of using the FLAG system is that any Hum4 V_(L)-V_(H) SCFV combination that has bound to TAG-72 will be detected. Affinities can be measured by Scatchard analysis (Scatchard (1949), supra) and specificity by immunohistochemistry. These canidates could then be checked for binding to a specific epitope by using the competition assay, supra, and a competing antibody or mimetic, if desired.

The present invention is not to be limited in scope by the cell lines deposited since the deposited embodiment is intended as two illustrations of one aspect of the invention and all cell lines which are functionally equivalent are within the scope of the invention. Indeed, while this invention has been described in detail and with reference to specific embodiments thereof, it will be apparent to one skilled in the art that various changes and modifications could be made therein without departing from the spirit and scope of the appended claims.

Example 6

Hum4 V_(L) may also be used as a source of framework regions (FRs) for grafting the complementarity determining regions (CDRs) of the light chain variable region of an antibody, such as the V_(L) of the TAG-72-specific antibody, CC49. When Hum4 V_(L) FRs are used in a humanized variable region construct (i.e. comprising non-human CDRs), the FRs may also be modified by replacing one or more of their amino acids with, e.g., murine, amino acids that may permit improvement in the functioning of the resulting antibody. Such an amino-acid-modified variable region is still considered a “humanized” region. An antibody or single chain antibody comprising a humanized light chain variable region having Hum4 V_(L) FRs is herein termed a “humanized Hum4 V_(L), V_(H) antibody,” i.e. any antibody or type of antibody in which the V_(L)(s) comprise (native or modified) Hum4 V_(L) FRs and CDRs grafted thereon which are, or are derived from, non-human CDRs.

A humanized Hum4 V_(L), V_(H) antibody may use, as the heavy chain variable region(s) thereof, a V_(H) which is entirely non-human, chimeric (partly human), humanized, or entirely human. Specifically in regard to αTAG-72 humanized Hum4 V_(L), V_(H) antibodies based on CC49, the V_(H) of such an antibody may be an entirely murine CC49 V_(H), a chimeric CC49 V_(H), or a humanized CC49 V_(H). The procedures set forth below describe production of an embodiment of the lattermost type of CC49-based αTAG-72 humanized Hum4 V_(L), V_(H) antibody: “HuCC49*” a humanized CC49 monoclonal humanized Hum4 V_(L), V_(H) antibody having CC49 V_(L) CDRs grafted upon Hum4 V_(L) FRs and having a humanized CC49 V_(H) region.

The specific light chain FRs chosen for use in humanizing the CC49 V_(L) are derived from the light chain FRs of the human MAb, LEN (the LEN light chain being a human k Subgroup IV light chain). This particular light chain was selected from among the human k Subgroup IV light chain sequences reported in Kabat et al., Sequences of Proteins of Immunological Interest (5th ed., 1991) (U.S. Department of Health and Human Services, NIH Publication No. 901-3242), based on the degree of similarity of its framework amino acid residues to certain framework residues of the native CC49 (nCC49) V_(L)—i.e. those residues potentially significant for maintenance of the combining site structure present in nCC49.

The decision as to which nCC49 amino acid residues were possibly significant, was itself based on study of a three-dimensional model of another antibody, McPC603, whose V_(L) amino acids display identity to 95 of the 113 residues of the nCC49 V_(L) (and identity to 69 of the 80 V_(L) FR residues thereof). See E. A. Padlan, Mol. Immunol., 31:169-217 (1994); however, the effects of specific amino acid residues and changes thereto are unpredictable. Based on this study, it was estimated that 43 of the nCC49 V_(L) FR residues were possibly significant (see FIG. 32A, asterisked residues), and the LEN V_(L) was selected because its FR amino acids displayed identity in 36 of these 43 residues.

The same decision-making process was used to select the specific heavy chain FRs to be used in humanizing the nCC49 V_(H). These FRs are derived from the heavy chain FRs of the human MAb, 21/28′CL, which was chosen based on a three-dimensional model of the antibody, 36-71. The V_(H) amino acids of 36-71 display identity to 84 of the 115 residues of the nCC49 V_(H), and identity to 71 of the 87 FR residues thereof. (See Padlan, ibid.) Based on the study of 36-71, it was estimated that 40 of the nCC49 V_(H) residues were possibly significant (see FIG. 32B, asterisked residues), and the 21/28′CL V_(L) was then selected because its FR amino acids displayed identity in 28 of these 40 residues.

Of the 7 remaining, non-identical “possibly significant” residues of the LEN V_(L) FRs, and the 12 of the 21/28′CL V_(L) FRs, these residues were replaced with the corresponding amino acids of CC49 V_(L) and CC49 V_(H), respectively, so as to retain in the final, humanized antibody, all of the residues estimated as being “possibly significant.” Thus, the humanized MAb, HuCC49*, was designed to comprise: 1) a humanized V_(L) comprising the three V_(L) CDRs of nCC49 and the residue-modified V_(L) FRs of the human MAb, LEN; and 2) a humanized V_(H) comprising the three V_(H) CDRs of nCC49 and the residue-modified V_(H) FRs of the human MAb, 21/28′CL. (See FIGS. 32A-B which sets forth the humanization protocols for the CC49 V_(L) and V_(H) regions.)

Based on the resulting humanization protocols, nucleotide sequences were deduced from the amino acid sequence of each of the humanized V_(L) and V_(H) regions. The projected sequences were then refined by choosing codons for high frequency usage in the murine system and also by eliminating—with the help of the programs FOLD and MAPSORT, (Devereux et al., Nucl. Acids Res., 12:387-395 (1984))—any self-annealing regions or any internal sites for the restriction endonucleases which were to be used for inserting the sequences into the appropriate vectors. The refined nucleotide sequences are shown in FIGS. 33A-B.

For the generation of humanized V_(H)- and V_(L)-coding sequences, two sets of four 121- to 126-base-pair-long oligonucleotides were synthesized. The four overlapping oligomers of a given set (depicted by long arrows in FIGS. 3A-B) encompassed the entire refined nucleotide sequence of the humanized V_(H) or V_(L) gene on alternating strands. Double-stranded coding sequences were generated from the overlapping oligomers and then amplified, by the polymerase chain reaction (PCR), according to the following procedures.

First, four 20-base-pair-long amplification end primers were purchased (Midland Certified Reagent Co., Midland, Tex.) or synthesized (using a model 8700 DNA synthesizer Milligen/Bioresearch, Burlington, Vt.), and then these end primers were chromatographically purified (on Oligo-Pak columns from Milligen/Bioresearch). The sequences of these end primers were:

1. 5′ V_(H), coding: 5′-CTAAGCTTCCACCATGGAGN-3′ (SEQ ID NO:36);

2. 3′ V_(H), noncoding: 5′-ATGGGCCCGTAGTTTTGGCG-3′ (SEQ ID NO:37);

3. 5′ V_(L), coding: 5′-GCAAGCTTCCACCATGGATA-3′ (SEQ ID NO:38); and

4. 3′ V_(L), noncoding: 5′-AGCCGCGGCCCGTTTCAGTT-3′ (SEQ ID NO:39).

Both of the 5′-primers carry a HindIII site, while the 3′ V_(H) primer has an ApaI site and the 3′ V_(L) primer carries a SacII site at the flank.

PCR was carried out separately for each of the V_(L) and V_(H) coding sequences (data not shown), according to standard PCR reaction procedures. (Daugherty et al., Nucl. Acids, Res., 19:2471-2476 (1991).) To a final volume of 50 ml of PCR buffer—containing 2.5 mM of each of the dNTPs and 2.5 units of Taq DNA polymerase (Perkin Elmer Cetus, Norwalk, Conn.)—1 pmol each of the four overlapping oligomers and 50 pmol each of the two end primers were added. Three cycles of denaturation (1 min at 94° C.), annealing (2 min at 55° C.), and polymerization (2 min at 70° C.) were followed by 17 additional cycles of denaturation (1 min at 94° C.), annealing (2 min at 55° C.), and polymerization (1 min at 72° C.). This was followed by a final primer extension for 15 min at 72° C.

The DNA was extracted with phenol/chloroform and precipitated with ethanol. The amplified DNA was gel purified either as such or after treatment with the appropriate restriction endonucleases. Then the purified, PCR-generated copies of the DNA sequence encoding the humanized V_(L) were cloned in the vector, pBluescript SK⁺ (Stratagene, La Jolla, Calif.), while those for the humanized V_(H) were separately cloned in the vector pCRII (a TA cloning vector designed for cloning PCR products, from Invitrogen, San Diego, Calif.) thereby generating pBSHuCC49*V_(L) and pTAHuCC49*V_(H), respectively. Each of the humanized variable regions was sequenced to check the fidelity of the PCR products.

After the fidelity of the PCR products was checked, eukaryotic expression vectors bearing genes comprising these variable region-encoding DNA sequences were constructed as illustrated in FIGS. 34A-B. The expression vectors bear a gene for a selectable marker. This gene for the selectable marker is driven by the 5′ long terminal repeat derived from M-MSV, while the human cytomegalovirus (HCMV) immediate early promoter drives the “target” gene, i.e. the HuCC49* light or heavy chain gene construct. A multiple cloning site is located immediately 3′ to the HCMV promoter.

For the light chain of HuCC49*, pLNCXCC49Huk—an expression construct of the cCC49 light chain—was used as a source of DNA encoding the human k constant region. Taking advantage of an internal SacII site and a ClaI site located 3¢ to the constant region DNA, a SacII/ClaI fragment encoding the human k constant region was excised therefrom. This fragment, together with the humanized V_(L)-encoding HindIII/SacII fragment excised from pBSHuCC49*VL, was inserted directionally, by three-way ligation, between HindIII and ClaI sites in the retroviral expression construct pLNCX II, a retroviral vector. This vector is essentially the vector pLNCX, (Miller et al., Biotechniques, 7:980-989 (1989)), obtained from Dr. D. Miller (Fred Hutchinson Cancer Research Center, Seattle, Wash.) and modified by destroying an EcoRI site in the backbone of the vector while retaining another EcoRI site located 45 base pairs 5′ to the neomycin resistance gene therein. pLNCX II is hereinafter referred to as pLNCX. Insertion of these two DNA fragments into pLNCX as indicated resulted in formation of pLNCXHuCC49HuK. (See FIG. 34A.)

For the heavy chain construct, an ApaI/ClaI DNA fragment encoding a human γ1 constant region was excised from pLHCXCC49HuG₁—an expression construct of the cCC49 heavy chain—by taking advantage of an ApaI site in the C_(H)1 domain of the human γ1 and a ClaI site located 3′ to the γ1 heavy chain. A HindIII/ApaI fragment encoding the humanized V_(H) region was obtained from the construct pTAHuCC49*V_(H). Again, three-way ligation was used to directionally clone the two DNA fragments between the HindIII and ClaI sites of an expression vector, pLgpCX II. pLgpCX II is a retroviral vector derived from pLNCX II by replacing a 1.2-kb BamHI fragment carrying the neomycin resistance gene with a 0.7-kb BglII/BamHI fragment carrying the Ecogpt gene which had been excised from the vector pEE6HCMVgpt, (White et al., Protein Eng., 1:499-505 (1987)). The Ecogpt gene encodes xanthine-guanine phosphoribosyltransferase which confers resistance to mycophenolic acid in mammalian cells grown in culture medium supplemented with xanthine. pLgpCX II is hereinafter referred to as pLgpCX. Insertion of these two DNA fragments into pLgpCX as indicated resulted in formation of pLgpCXHuCC49HuG₁. (See FIG. 34B.)

In order to express the HuCC49* MAb itself, the pLNCXHuCC49HuK and pLgpCXHuCC49HuG₁ expression vector constructs were sequentially transfected into host cells as follows.

First, the expression construct, pLNCXHuCC49HuK, was electroporated into SP2/0 murine myeloma cells (of the SP2/0-Ag14 cell line, obtained as a gift from Dr. S. Morrison, University of California, Los Angeles), using the Cell-Porator system (GIBCO BRL, Gaithersburg, Md.). Electroporation was carried out as previously described (Slavin-Chiorini et al., Int. J. Cancer, 53:97-103 (1993)), with minor modifications. Briefly, 40 mg of the PvuI linearized DNA was added to a polypropylene electroporation chamber containing 1×10⁷ cells suspended in 1 ml of serum-free DMEM supplemented with 4.5 g/liter glucose. The cell/DNA mixture was placed in an ice-water bath and pulsed at 650 V/cm for 13 msec at a capacitance setting of 1600 mF. After keeping the cells on ice for 10 min, they were diluted in complete RPMI-1640 medium [RPMI-1640 containing 15% (v/v) heat-inactivated fetal calf serum, 2 mM L-glutamine, 1 mM sodium pyruvate, and 50 mg gentamicin/ml] and distributed in 24-well tissue culture plates (Costar, Cambridge, Mass.) at 1×10⁵ cells/wells. After incubation at 37° C. in a 5% CO₂ incubator for 48 h, the medium was replaced with selection medium.

Selection medium consisted of complete RPMI-1640 containing 700 mg/ml of active G418 (GIBCO BRL). After 2 weeks of selection in medium supplemented with G418, approximately 20% of the wells showed cell growth. Tissue culture supernatants from approximately 50% of the wells with cell growth were positive for human k chain reactivity, indicating that these cells were expressing the k light chain of HuCC49*.

Second, the expression construct pLgpCXHuCC49HuG₁, was electroporated into a HuCC49* k chain-producing transfectant using the above-described procedure. However, after electroporation and incubation, the medium was instead replaced with a selection medium consisting of complete RPMI-1640 containing 1 mg/ml mycophenolic acid (Sigma Chemical Co., St. Louis, Mo.), 250 mg/ml xanthine, and 15 mg/ml hypoxanthine (GIBCO BRL). After selection in this mycophenolic acid-containing medium, supernatants from two transfectants were reactive with a protein extract of TAG-72-positive LS-174T human colon carcinoma xenografts, indicating that these cells were expressing a whole αTAG-72 antibody.

In addition, no reactivity was observed to a protein extract of TAG-72-negative A375 human melanoma xenografts (the A375 human melanoma cell line being obtained from Dr. S. Aaronson, National Cancer Institute, NIH, Bethesda, Md.), thereby indicating that these two transfectants were expressing an antibody specific for TAG-72. The transfectant that secreted a higher titer of TAG-72-reactive Ig was cloned by limiting dilution, and the subclone that produced the highest titer—designated HuCC49*—was adapted for growth in serum- and protein-free medium (PFHM-II, Gibco BRL).

In order to assess the purity of the HuC49* antibody, and to characterize its mobility relative to that of chimeric CC49 (cCC49), SDS-PAGE analysis was performed under reducing and non-reducing conditions. Quantities of the HuCC49* antibody sufficient for these analyses were obtained by growing the above-selected HuCC49* clone in protein-free hybridoma medium PFHM-II (Gibco BRL), followed by isolation from the tissue culture supernatants via protein G affinity chromatography and concentration of the harvested antibody as follows: 1) the tissue culture supernatants were applied to a recombinant protein G (Gibco BRL) agarose column; 2) the bound protein was eluted from the column using 0.1 M glycine hydrochloride buffer, pH 2.6; 3) the pH of the eluted material was immediately adjusted to 7.0 using 1.0 M Tris buffer, pH 8.0; 4) the pH 7.0 material was dialyzed against phosphate-buffered saline (PBS), pH 7.4; and 5) the dialyzed material was concentrated using an immersible-CX-30 ultrafilter (Millipore, Bedford, Mass.).

The HuCC49* protein concentration was determined using a Bio-Rad Microassay procedure, (M. M. Bradform, Anal. Biochem., 72:248-254 (1976)), or by the method of Lowry et al. (J. Biol. Chem., 193:265-275 (1951)). Approximately 1 mg of HuCC49* was recovered per ml of the tissue culture supernatants. cCC49 was purified from tissue culture supernatant using high-performance liquid chromatography and the protein concentration was likewise determined.

PAGE analyses of cCC49 and HuCC49* were performed on precast 4-20% SDS-polyacrylamide Tris-glycine gels (Novex, San Diego, Calif.) with and without denaturation with 2-mercaptoethanol. Proteins on the gel were visualized by staining with Coomassie Brilliant blue R250 according to the method of U. K. Laemmli (Nature (London), 227:680-685 (1970)).

The gel profiles under non-reducing conditions showed that the HuCC49* MAb (FIG. 35A, lane 2) has virtually identical mobility to that of cCC49 (FIG. 35A, lane 1), which has a molecular mass of approximately 160 kDa. Under reducing conditions, the HuCC49* MAb (FIG. 35B, lane 2) yielded two protein bands of approximately 25-28 and 50-55 kDa. This is consistent with the molecular masses of the heavy and light chains of cCC49 (FIG. 35B, lane 1).

In order to better characterize HuCC49* relative to cCC49 and nCC49, purified HuCC49*, cCC49, and nCC49 were obtained and radio-labeled for use in further analysis by PAGE, HPLC, and immunoreactivity studies (the development and reactivity of nCC49 has been previously described by Kuroki et al., Cancer Res., 48:4588-4596 (1988)). Thus, these three antibodies were labeled with Na¹²⁵I or Na¹³¹I using the Iodo-Gen (Pierce, Rockford, Ill.) method of Colcher et al. (Cancer Res., 25 43:736-742 (1983)). The iodination protocol resulted in ¹²⁵I-labeled cCC49, 125I-labeled nCC49, and ¹³¹I-labeled HuCC49* with specific activities of 2-5 mCi/mg.

These three radioiodinated antibodies were evaluated by SDS-PAGE analysis under non-reducing and reducing conditions. The radioiodinated MAbs were detected by autoradiography using KODAK XAR X-ray film (Eastman Kodak Co., Rochester, N.Y.) and LIGHTNING PLUS intensifying screens (E.I. DuPont deNemours & Co., Wilmington, Del.). Molecular weight profiles, similar to those described for the unlabeled purified MAbs, were observed.

The integrity of each of the radioiodinated CC49 molecules was then evaluated by HPLC size-exclusion chromatography. The HPLC analyses were performed using a SPHEROGEL-TSK 2000 SW, 0.75×30 cm column (Beckman Instruments, Inc., Berkeley, Calif.) equilibrated in 67 mM sodium phosphate containing 100 mM KCl, pH 6.8. Samples (250,000 cpm in 25 ml) were applied and eluted from the column at a flow rate of 0.5 ml/min. The radioactivity was measured in a flow-through gamma scintillation counter (Model 170; Beckman Instruments, Inc.). Each of ¹³¹I-labeled HuCC49*, ¹²⁵I-labeled cCC49, and ¹²⁵I-labeled nCC49 eluted from the column at 29 min. as a distinct species (see FIGS. 36A-B: data not shown for ¹²⁵I-labeled nCC49).

Finally, the immunoreactivities of the radiolabeled antibodies were assessed by a modification of a method previously described by Schott et al. (Cancer Res., 52:6413-5417 (1982)), using bovine submaxillary mucin (BSM). BSM was immobilized onto solid support gel beads (REACTI-GEL HW65F from Pierce, Rockford, Ill.) as detailed by Jonson et al. (Cancer Res., 46:850-857 (1986)), at a ratio of 2 mg BSM to 1 ml of wet-packed gel, and the TAG-72-positive BSM beads were used to perform the radioimmuno-reactivity assay. 50 ml of wet-packed BSM beads was placed in each tube of (multiple sets of) three pairs of 1.5 ml microfuge tubes. The radiolabeled antibodies were diluted to 23 nCi in 1 ml of 1% bovine serum albumin (BSA) in PBS. The radiolabeled antibodies were then added to the duplicate tubes, counted in a gamma scintillation counter, and incubated for 2 h at room temperature with end-over-end rotation. The BSM beads were then pelleted at 800×g for 5 min, and the beads in each tube were washed twice with 1 ml of 1% BSA in PBS. The radioactivity remaining in each tube was measured and the total percent bound to the BSM beads was calculated. The percent bound for each of the radiolabeled Ig forms was greater than 85, while the percent bound for a nonspecific antibody was typically <10%. Approximately 85% of the ¹³¹I-labeled HuCC49* and 90% of the ¹²⁵I-labeled cCC49 MAbs bound to BSM beads, thus indicating the immunoreactivity of the HuCC49* and cCC49 MAbs.

Next, the relative affinity constants (K_(a)) of HuCC49*, and cCC49 and nCC49, were determined using a competition radioimmunoassay (RIA) technique. Competition RIAs were performed using ¹²⁵I-labeled nCC49 as the radiolabeled antibody and BSM as the target antigen, according to the method of Hand et al. (Cancer Immunol. Immunother., 35:165-174 (1992)). In these RIAs, ¹²⁵I-labeled nCC49 was used to compete for the binding of each of the three unlabeled competitor antibodies bound to the TAG-72-positive BSM. The percentage of radiolabeled MAb bound to antigen (% bound), compared to a buffer control was calculated.

As shown in FIG. 37, all three CC49 MAbs were able to completely inhibit the binding of the ¹²⁵I-labeled nCC49 to TAG-72, although the level of competition observed with the HuCC49* MAb differed from that of the nCC49 and cCC49 MAbs. Approximately 45 mg of the HuCC49* was required for 50% competition, as compared with 1.5 and 2.0 mg of the nCC49 and cCC49 MAbs, respectively. No competition was observed when control murine IgG (MOPC-21, an IgG₁ murine myeloma protein obtained from Organon Technika, Durham, N.C.) and control human IgG (purified IgG obtained from Jackson Immuno-Research, West Grove, Pa.) were used as competitors.

The K_(as) of HuCC49*, cCC49, and nCC49 were determined using the method of G. Scatchard (Ann. NY Acad. Sci., 51:660-668 (1949)). The relative affinity constant of the cCC49 MAb was found to be 1.2×10⁸ M⁻¹ and that of the nCC49 MAb was found to be 1.8×10⁸ M⁻¹, while the K_(a) of HuCC49* was 6.0×10⁷ M⁻¹, i.e. approximately 2- to 3-fold less than those of the nCC49 and cCC49 MAbs, respectively.

Studies were then initiated to compare the plasma clearance and biodistribution of HuCC49* with that of cCC49. It has previously been shown that the pharmacokinetics of nCC49 and cCC49 in mice differ greatly, with cCC49 clearing more rapidly. This has been shown to affect in vivo tumor targeting. Thus, HuCC49* and cCC49 were compared in pharmacokinetics and in vivo targeting studies as follows. Each of 5 athymic (nu/nu) mice received one i.v. injection containing a mixture of 0.94 mCi/mouse of ¹²⁵I-labeled cCC49 and 0.98 mCi/mouse of ¹³¹I-labeled HuCC49*. Blood samples were collect at specified time intervals via the tail vein into 10-ml capillary tubes (Drummon, Broomall, Pa.). The amount of ¹³¹I and ¹²⁵I in the plasma was determined and normalized to account for differences in the rates of decay of the radionuclides. The percentage of the injected dose of each radionuclide remaining the plasma was then calculated.

The data from this experiment was used to determine the half-lives of the antibodies in plasma, using a curve-fitting program. The t_(1/2)a and t_(1/2)b of HuCC49* were 4.2 and 149 h, respectively. These values are comparable to the t_(1/2)a and t_(1/2)b values for cCC49, i.e. 4.7 and 139 h, respectively. Statistical analysis was also performed on this plasma clearance data using a 2-tailed paired Student's t test with n=5 and 4 degrees of freedom. FIG. 38 shows that both MAbs have similar blood clearance patterns with approximately 74% of the radiolabeled MAb dose clearing the blood at 24 h and 83% by 72h.

Experiments were then conducted to assess the biodistribution of these antibodies in order to compare the ability of HuCC49* to localize to human tumor xenografts with that of cCC49. The biodistribution of the HuCC49* MAb was compared with that of the cCC49 MAb as follows. Female athymic (nu/nu) mice bearing TAG-72-positive tumor xenografts were produced according a method previously described by Colcher et al. (Cancer Res., 43:736-742 (1983)), using cells from the LS-174T human colon adenocarcinoma cell line (described by Rutzki et al., In Vitro, 12:180-191 (1976)) which was obtained from the American Type Culture Collection (Rockville, Md.).

These tumor-bearing mice were injected in the tail vein with a mixture containing approximately 0.94 mCi/mouse of ¹²⁵I-labeled cCC49 and 0.98 mCi/mouse of ¹³¹I-labeled HuCC49*. Blood, tumor, and all major organs were collected and weighted (5 mice per time point). Radioactivity was measured in a gamma scintillation counter and radioactive decay was counted. The percentage of the injected dose per gram (% ID/g) for each organ was determined and the radiolocalization indices (%ID/g in tumor divided by the %ID/g in normal tissue) were calculated. Statistical analysis was also done for the biodistribution data using a 2-tailed paired Student's t test with n=5 and 4 degrees of freedom.

No statistically significant difference was observed between the %ID/g of either MAb to tumors or tissues collected at any time point (Table 2). Both antibodies showed tumor localization by 24 h; by 96 h, when there was <2% of the injected dose per ml of blood, the %ID/g in tumor was 22.6% for HuCC49* and 19.5% for cCC49 (Table 2). No specific uptake of either radiolabeled antibody was observed in any normal tissue tested. As shown in Table 3, the radiolocalization indices (RIs) (%ID/g in tumor divided by the %ID/g in normal tissue) of the two MAbs were not appreciably different for any tumor:normal tissue ratio. Thus, these data indicate that the HuCC49* and cCC49 MAbs have similar tumor-targeting properties.

TABLE 2 Radiolocalization of ¹³¹I-Labeled HuCC49* and ¹²⁵I-Labeled CCC49 in Athymic Mice Bearing LS-174T Tumors (% ID/g)^(a) Antibody, % ID/g Organ 24 h 48 h 72 h 96 h 168 h HuCC49* Tumor 15.4 ± 7.2^(b) 22.8 ± 17.1 16.1 ± 12.8 22.6 ± 5.3  12.9 ± 12.4 Blood 5.1 ± 1.4 4.1 ± 2.9 2.2 ± 1.8 1.6 ± 0.8 0.8 ± 0.7 Liver 3.3 ± 1.6 2.7 ± 0.9 1.5 ± 0.8 1.6 ± 0.2 0.6 ± 0.3 Spleen 5.7 ± 3.9 4.7 ± 0.9 2.7 ± 1.6 4.6 ± 0.8 3.3 ± 2.5 Kidneys 1.5 ± 0.4 1.1 ± 0.4 0.8 ± 0.5 0.6 ± 0.1 0.4 ± 0.2 Lungs 2.0 ± 0.3 2.0 ± 1.2 1.0 ± 0.7 1.0 ± 0.4 0.4 ± 0.3 cCC49 Tumor 16.1 ± 7.5  23.4 ± 19.0 14.9 ± 12.0 10.5 ± 6.0  12.3 ± 11.3 Blood 4.5 ± 1.6 3.6 ± 2.8 1.7 ± 1.4 1.1 ± 0.7 0.6 ± 0.4 Liver 3.8 ± 1.8 3.0 ± 0.8 1.4 ± 0.9 1.3 ± 0.2 0.4 ± 0.3 Spleen 8.5 ± 5.5 6.7 ± 1.2 3.5 ± 2.5 7.5 ± 2.7 4.8 ± 3.8 Kidneys 1.6 ± 0.4 1.0 ± 0.5 0.6 ± 0.4 0.4 ± 0.1 0.3 ± 0.1 Lungs 2.1 ± 0.3 1.9 ± 1.2 0.8 ± 0.5 0.8 ± 0.4 0.4 ± 0.2 ^(a)Athymic mice (5 per group) bearing LS-174T human colon carcinoma xenografts were injected i.v. with a mixture containing approximately 10 mCi of each radiolabeled MAb, and sacrificed at the indicated times. ^(b)Values represent the mean % ID/g ± SD of samples from 5 mice.

TABLE 3 Radiolocalization of ¹³¹I-Labeled HuCC49* and ¹²⁵I-Labeled cCC49 in Athymic Mice Bearing LS-174T Tumors (Radiolocalization Index)^(a) Antibody, Radiolocalization Index^(b) Organ 24 h 48 h 72 h 96 h 168 h HuCC49* Blood  3.1 ± 1.1^(c) 6.8 ± 4.1 8.6 ± 3.7 16.7 ± 5.5 24.7 ± 23.6 Liver 5.4 ± 3.3 9.2 ± 7.4 9.2 ± 3.9 14.1 ± 3.9 21.8 ± 14.3 Spleen 3.6 ± 2.7 5.3 ± 4.5 5.4 ± 2.9  5.0 ± 1.6 5.1 ± 5.1 Kidneys 10.2 ± 4.8  19.5 ± 8.6  18.2 ± 5.5  37.7 ± 3.2 30.6 ± 20.4 Lungs 7.7 ± 3.1 11.4 ± 4.3  14.6 ± 3.2  25.5 ± 7.3 36.6 ± 27.6 cCC49 Blood 3.8 ± 1.4 9.1 ± 7.0 12.1 ± 7.1  21.0 ± 6.9 27.3 ± 23.8 Liver 4.7 ± 2.6 8.2 ± 6.6 9.3 ± 2.6 14.6 ± 3.9 25.8 ± 17.0 Spleen 2.4 ± 1.6 3.5 ± 2.5 4.4 ± 2.4  2.9 ± 1.4 3.6 ± 3.4 Kidneys 10.2 ± 4.7  20.4 ± 8.0  21.5 ± 5.3  46.1 ± 7.5 40.6 ± 26.9 Lungs 7.9 ± 3.5 11.9 ± 4.4  16.0 ± 3.7  26.6 ± 9.1 32.2 ± 22.7 ^(a)Athymic mice (5 per group) bearing LS-174T human colon carcinoma xenografts were injected i.v. with a mixture containing approximately 1.0 μCi of each radiolabeled MAb, and sacrificed at the indicated times. ^(b)The radiolocalization index is the ratio of the % ID/g of the tumor tissue to the % ID/g of the normal tissue. ^(c)Values represent the radiolocalization index ± SD of samples from 5 mice.

Other embodiments of the invention will be apparent to those skilled in the art from a consideration of this specification or practice of the invention disclosed herein. It is intended that the specification and examples be considered as exemplary only, with the true scope and spirit of the invention being indicated by the following claims.

78 1 70 DNA Artificial Sequence Primer SCFV1a 1..70 Oligonucleotide for incorporating part of the yeast invertase leader sequence into DNA encoding SCFV1 1 ctgcaagctt ccttttcctt ttggctggtt ttgcagccaa aatatctgca gacatcgtga 60 tgacccagtc 70 2 81 DNA Artificial Sequence Primer SCFV1b 1..81 Oligonucleotide for incorporating the SCFV linker coding sequence into DNA encoding SCFV1 2 cgtaagacgt ctaaggaacg aaattgggcc aattgttctg aggagaccga acctgactcc 60 ttcaccttgg tccctccgcc g 81 3 30 DNA Artificial Sequence Primer SCFV1c 1..30 Oligonucleotide for amplifying CC49 VH 3 cttagacgtc cagttgcagc agtctgacgc 30 4 37 DNA Artificial Sequence Primer SCFV1d 1..36 Oligonucleotide for amplifying CC49 VH 4 gatcaaggct tcactaggag acggtgactg aggttcc 37 5 24 DNA Artificial Sequence Primer TPI 1..24 Oligonucleotide used to sequence DNA encoding SCFV1 5 caattttttg tttgtattct tttc 24 6 20 DNA Artificial Sequence Primer HUVKF3 1..20 Oligonucleotide used to sequence DNA encoding SCFV1 6 cctgaccgat tcagtggcag 20 7 27 DNA Artificial Sequence Primer DC113 1..27 Oligonucleotide used to sequence DNA encoding SCFV1 7 tccaatccat tccaggccct gttcagg 27 8 20 DNA Artificial Sequence Primer SUC2T 1..20 Oligonucleotide used to sequence DNA encoding SCFV1 8 cttgaacaaa gtgataagtc 20 9 26 DNA Artificial Sequence Primer penP1 1..26 Oligonucleotide for amplifying the penP promoter - pel B signal sequence of plasmid pRW83 9 cgataagctt gaattccatc acttcc 26 10 90 DNA Artificial Sequence Primer penP2 1..90 Oligonucleotide for amplifying the penP promoter - pel B signal sequence of plasmid pRW83 10 ggccatggct ggttgggcag cgagtaataa caatccagcg gctgccgtag gcaataggta 60 tttcatcaaa atcgtctccc tccgtttgaa 90 11 44 DNA Artificial Sequence Primer penP3 1..44 Forward oligonucleotide primer for amplifying DNA encoding a Hum4VL-linker-CC49VH SCFV from pCGS515/SCFV1 11 gctgcccaac cagccatggc cgacatcgtg atgacccagt ctcc 44 12 90 DNA Artificial Sequence Primer penP6(-) 1..90 Reverse oligonucleotide primer for amplifying DNA encoding a Hum4VL-linker-CC49VH SCFV from pCGS515/SCFV1 12 ctcttgatca ccaagtgact ttatgtaaga tgatgttttg acggattcat cgcaatgttt 60 ttatttgccg gagacggtga ctgaggttcc 90 13 23 DNA Artificial Sequence Primer Nco1.1 1..23 Forward oligonucleotide primer for destroying the Nco I site within the Camr gene in plasmid pSCFV31 13 tccggaattc cgtatggcaa tga 23 14 37 DNA Artificial Sequence Primer Nco1.3(-) 1..37 Reverse oligonucleotide primer for destroying the Nco I site within the Camr gene in plasmid pSCFV31 14 cttgcgtata atatttgccc atcgtgaaaa cgggggc 37 15 22 DNA Artificial Sequence Primer Nco1.2 1..22 Forward oligonucleotide primer for destroying the Hind III site at the 5′ end of the penP promoter in plasmid pSCFV31 15 atgggcaaat attatacgca ag 22 16 36 DNA Artificial Sequence Primer Nco1.4c(-) 1..36 Reverse oligonucleotide primer for destroying the Hind III site at the 5′ end of the penP promoter in plasmid pSCFV31 16 cactgaattc atcgatgata agctgtcaaa catgag 36 17 35 DNA Artificial Sequence Primer 104BH1 1..35 Forward oligonucleotide primer for amplifying DNA encoding Hum4VL from plasmid pCGS515/SCFV1 17 cagccatggc cgacatcgtg atgacccagt ctcca 35 18 86 DNA Artificial Sequence Primer 104BH2(-) 1..86 Reverse oligonucleotide primer for amplifying DNA encoding Hum4VL from plasmid pCGS515/SCFV1 18 aagcttgccc catgctgctt taacgttagt tttatctgct ggagacagag tgccttctgc 60 ctccaccttg gtccctccgc cgaaag 86 19 50 DNA Artificial Sequence Primer 104B3 1..50 Forward oligonucleotide primer for amplifying DNA encoding CC49 VH from plasmid p49g1-2.3 19 gttaaagcag catggggcaa gcttatgact cagttgcagc agtctgacgc 50 20 99 DNA Artificial Sequence Primer 104B4(-) 1..99 Reverse oligonucleotide primer for amplifying DNA encoding CC49 VH from plasmid p49g1-2.3 20 ctcttgatca ccaagtgact ttatgtaaga tgatgttttg acggattcat cgctagcttt 60 ttatttgcca taataagggg agacggtgac tgaggttcc 99 21 34 DNA Artificial Sequence Primer UNIH1 1..34 Forward oligonucleotide primer for amplifying DNA encoding Hum4VL from plasmid pRL1001 21 cagccatggc cgacattgtg atgtcacagt ctcc 34 22 72 DNA Artificial Sequence Primer UNIH2(-) 1..72 Reverse oligonucleotide primer for amplifying DNA encoding Hum4VL from plasmid pRL1001 22 gaggtccgta agatctcgcc tcgctaccta gcaaaaggtc ctcaagcttg atcaccacct 60 tggtccctcc gc 72 23 48 DNA Artificial Sequence Primer UNI3 1..48 Forward oligonucleotide primer for amplifying DNA encoding CC49 VH from plasmid p49g1-2.3 23 agcgaggcag atcttacgga cctcgaggtt cagttgcagc agtctgac 48 24 40 DNA Artificial Sequence Primer UNI4(-) 1..40 Reverse oligonucleotide primer for amplifying DNA encoding CC49 VH from plasmid p49g1-2.3 24 catcgctagc tttttatgag gagacggtga ctgaggttcc 40 25 89 DNA Artificial Sequence Primer UNIHOPE 1..89 Oligonucleotide encoding the (+) strand of the linker UNIHOPE 25 tataaagctt agtgcggacg atgcgaaaaa ggatgctgcg aagaaggatg acgctaagaa 60 agacgatgct aaaaaggacc tcgagtcta 89 26 89 DNA Artificial Sequence Primer UNIHOPE(-) 1..89 Oligonucleotide encoding the (-) strand of the linker UNIHOPE 26 tagactcgag gtccttttta gcatcgtctt tcttagcgtc atccttcttc gcagcatcct 60 ttttcgcatc gtccgcacta agctttata 89 27 30 DNA Artificial Sequence Primer HVH135 1..30 Forward oligonucleotide primer for amplifying human VH genes 27 tattctcgag gtgcarctgs tgsagtctgg 30 28 30 DNA Artificial Sequence Primer HVH2A 1..30 Forward oligonucleotide primer for amplifying human VH genes 28 tattctcgag gtcaasttra rggagtctgg 30 29 30 DNA Artificial Sequence Primer HVH46 1..30 Forward oligonucleotide primer for amplifying human VH genes 29 tattctcgag gtacagctrc agswgtcvgg 30 30 30 DNA Artificial Sequence Primer JH1245 1..30 Reverse oligonucleotide primer for amplifying human VH genes 30 ttatgctagc tgaggagacr gtgaccaggg 30 31 30 DNA Artificial Sequence Primer JH3 1..30 Reverse oligonucleotide primer for amplifying human VH genes 31 ttatgctagc tgaagagacg gtgaccattg 30 32 30 DNA Artificial Sequence Primer JH6 1..30 Reverse oligonucleotide primer for amplifying human VH genes 32 ttatgctagc tgaggagacg gtgaccgtgg 30 33 50 DNA Artificial Sequence Primer FLAGC 1..50 Forward oligonucleotide primer for generating plasmid pATDFLAG from plasmid pSCFVUHH 33 tcgagacaat gtcgctagcg actacaagga cgatgatgac aaataaaaac 50 34 50 DNA Artificial Sequence Primer FLAGNC 1..50 Reverse oligonucleotide primer for generating plasmid pATDFLAG from plasmid pSCFVUHH 34 ctaggttttt atttgtcatc atcgtccttg tagtcgctag cgacattgtc 50 35 22 DNA Artificial Sequence Primer PENPTSEQ 1..22 Oligonucleotide primer for verifying the sequence of the FLAG linker in pATDFLAG 35 ctttatgtaa gatgatgttt tg 22 36 20 DNA Artificial Sequence Primer 5 VH, coding 1..20 Forward oligonucleotide primer for generating DNA encoding a humanized VH 36 ctaagcttcc accatggagn 20 37 20 DNA Artificial Sequence Primer 3 VH, noncoding 1..20 Reverse oligonucleotide primer for generating DNA encoding a humanized VH 37 atgggcccgt agttttggcg 20 38 20 DNA Artificial Sequence Primer 5 VL, coding 1..20 Forward oligonucleotide primer for generating DNA encoding a humanized VL 38 gcaagcttcc accatggata 20 39 20 DNA Artificial Sequence Primer 3 VL, noncoding 1..20 Reverse oligonucleotide primer for generating DNA encoding a humanized VL 39 agccgcggcc cgtttcagtt 20 40 982 DNA Mus musculus VHaTAG germline coding sequence part 1 384..428 Intron 429..508 VHaTAG germline coding sequence part 2 509..813 40 ccttctcttc ctccaccacc aaatccacca tttgtaaatc aacatgttaa catatcacag 60 agtggagcaa cagaatcagg gcaaaaatat gctgagagat ttatccctgt cgttacaacc 120 aaagcatctg tctagaattc ataaaaactt tatgggatac atttcctcag agaggaatag 180 gatttggacc tgacgatcct gctgcccgag ccatgtgatg acagttcttc tccagttgaa 240 ctaggtcctt atctaagaaa tgcactgctc atgaatatgc aaatcacccg agtctatggc 300 agtaaataca gagatgttca taccataaaa acaatatatg atcagtgtct tctccgctat 360 ccctggacac actgactcta acc atg gaa tgg agc tgg gtc ttt ctc ttc ttc 413 Met Glu Trp Ser Trp Val Phe Leu Phe Phe 1 5 10 ctg tca gta act aca g gtaaggggct caccatttcc aaatctaaag tggagtcagg 469 Leu Ser Val Thr Thr 15 gcctgaggtg acaaagatat cacctttggc tttccacag gt gtc cac tcc cag gtt 525 Gly Val His Ser Gln Val 20 cag ctg cag cag tct gac gct gag ttg gtg aaa cct ggg gct tca gtg 573 Gln Leu Gln Gln Ser Asp Ala Glu Leu Val Lys Pro Gly Ala Ser Val 25 30 35 aag ata tcc tgc aag gct tct ggc tac acc ttc act gac cat gct att 621 Lys Ile Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Asp His Ala Ile 40 45 50 cac tgg gtg aag cag aag cct gaa cag ggc ctg gaa tgg att gga tat 669 His Trp Val Lys Gln Lys Pro Glu Gln Gly Leu Glu Trp Ile Gly Tyr 55 60 65 att tct ccc gga aat ggt gat att aag tac aat gag aag ttc aag ggc 717 Ile Ser Pro Gly Asn Gly Asp Ile Lys Tyr Asn Glu Lys Phe Lys Gly 70 75 80 85 aag gcc aca ctg act gca gac aaa tcc tcc agc act gcc tac atg cag 765 Lys Ala Thr Leu Thr Ala Asp Lys Ser Ser Ser Thr Ala Tyr Met Gln 90 95 100 ctc aac agc ctg aca tct gag gat tct gca gtg tat ttc tgt aaa aga 813 Leu Asn Ser Leu Thr Ser Glu Asp Ser Ala Val Thr Phe Cys Lys Arg 105 110 115 cacagtgttg taaccacatc ctgagtgtgt cagaaatcct gggggagcag aaagatacac 873 tgggactgag aagacagaaa aattaatcct tagacttgct cagaaatcgt aattttgaat 933 gcctatttat ttcatcttgc tcacacacct atattgcttt tgtaagctt 982 41 451 DNA Mus musculus CC46 VH coding sequence 49..450 41 atatgatcag tgtcttctcc gctatccctg gacacactga ctctaacc atg gaa tgg 57 Met Glu Trp 1 agc tgg gtc ttt ctc ttc ttc ctg tca gta act aca ggt gtc cac tcc 105 Ser Trp Val Phe Leu Phe Phe Leu Ser Val Thr Thr Gly Val His Ser 5 10 15 caa ttt cag cta cag cag tct gac gct gag ttg gtg aga cct ggg gct 153 Gln Phe Gln Leu Gln Gln Ser Asp Ala Glu Leu Val Arg Pro Gly Ala 20 25 30 35 tca gtg aag att tcc tgc aag gct tct ggc tac acc ttc act gac cat 201 Ser Val Lys Ile Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Asp His 40 45 50 gct att cac tgg gtg aag cag aag cct gaa cag ggc ctg gaa tgg att 249 Ala Ile His Trp Val Lys Gln Lys Pro Glu Gln Gly Leu Glu Trp Ile 55 60 65 gga tat ttt tct ccc gga aat ggt gat att aag tac aat gag aag ttc 297 Gly Tyr Phe Ser Pro Gly Asn Gly Asp Ile Lys Tyr Asn Glu Lys Phe 70 75 80 aag ggc aag gcc aca ctg act gca gac aaa tcc tcc agc act gcc tac 345 Lys Gly Lys Ala Thr Leu Thr Ala Asp Lys Ser Ser Ser Thr Ala Tyr 85 90 95 atg cag ttc aac agc ctg aca tct gag gac tct gca gtg tat ttc tgt 393 Met Gln Phe Asn Ser Leu Thr Ser Glu Asp Ser Ala Val Thr Phe Cys 100 105 110 115 acg ggc ggc tac ggg ttt gct ttc tgg ggc caa ggg act ctg gtc act 441 Thr Gly Gly Tyr Gly Phe Ala Phe Trp Gly Gln Gly Thr Leu Val Thr 120 125 130 gtc tct gca g 451 Val Ser Ala 42 865 DNA Mus musculus CC49 VH coding sequence part 1 384..428 Intron 429..508 CC49 VH coding sequence part 2 509..864 42 ccttctcttc ctccaccacc aaatccacca tttgtaaatc aacatgttaa catatcacag 60 agtggagcaa cagaatcagg gcaaaaatat gctgagagat ttttccctgt cgttacaacc 120 aaagcatctg tctagaattc ataaaaactt tatgggatac atttcctcag agaggaatag 180 gatttggacc tgacgatcct gctgcccgag ccatgtgatg acagttcttc tccagttgaa 240 ctaggtcctt atctaagaaa tgcactgctc atgaatatgc aaatcacccg agtctatggc 300 agtaaataca gagatgttca taccataaaa acaatatgtg atcagtgtct tctccgctat 360 ccctggacac actgactcta acc atg gaa tgg agc tgg gtc ttt ctc ttc ttc 413 Met Glu Trp Ser Trp Val Phe Leu Phe Phe 1 5 10 ctg tca gta act aca g gtaaggggct caccatttcc aaatctaaag tggagtcagg 469 Leu Ser Val Thr Thr 15 gcctgaggtg acaaagatgt cacctttggc tgtccacag gt gtc cac tcc cag gtt 525 Gly Val His Ser Gln Val 20 cag ttg cag cag tct gac gct gag ttg gtg aaa cct ggg gct tca gtg 573 Gln Leu Gln Gln Ser Asp Ala Glu Leu Val Lys Pro Gly Ala Ser Val 25 30 35 aag att tcc tgc aag gct tct ggc tac acc ttc act gac cat gca att 621 Lys Ile Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Asp His Ala Ile 40 45 50 cac tgg gtg aaa cag aac cct gaa cag ggc ctg gaa tgg att gga tat 669 His Trp Val Lys Gln Asn Pro Glu Gln Gly Leu Glu Trp Ile Gly Tyr 55 60 65 ttt tct ccc gga aat gat gat ttt aaa tac aat gag agg ttc aag ggc 717 Phe Ser Pro Gly Asn Asp Asp Phe Lys Tyr Asn Glu Arg Phe Lys Gly 70 75 80 85 aag gcc aca ctg act gca gac aaa tcc tcc agc act gcc tac gtg cag 765 Lys Ala Thr Leu Thr Ala Asp Lys Ser Ser Ser Thr Ala Tyr Val Gln 90 95 100 ctc aac agc ctg aca tct gag gat tct gca gtg tat ttc tgt aca aga 813 Leu Asn Ser Leu Thr Ser Glu Asp Ser Ala Val Thr Phe Cys Thr Arg 105 110 115 tcc ctg aat atg gcc tac tgg ggt caa gga acc tca gtc acc gtc tcc 861 Ser Leu Asn Met Ala Tyr Trp Gly Gln Gly Thr Leu Val Thr Val Ser 120 125 130 tca g 865 Ser 43 862 DNA Mus musculus CC83 VH coding sequence part 1 374..428 Intron 429..508 CC83 VH coding sequence part 2 509..861 43 ccttctcttc ctccaccacc aaatccacca tttgtaaatc aacatgttaa catatcacag 60 agtggagcaa cagaatcagg gcaaaaatat gctgagagat ttatccctgt cgttacaacc 120 aaagcatctg tctagaattc ataaaaactt tatgggatac atttcctcag agaggaatag 180 gatttggacc tgacgatcct gctgcccgag ccatgtgatg acagttcttc tccagttgaa 240 ctaggtcctt atctaagaaa tgcactgctc atgaatatgc aaatcacccg agtctatggc 300 agtaaataca gagatgttca taccataaaa acaatatatg atcagtgtct tctccgctat 360 ccctggacac actgactcta acc atg gaa tgg agc tgg gtc ttt ctc ttc ttc 413 Met Glu Trp Ser Trp Val Phe Leu Phe Phe 1 5 10 ctg tca gta act aca g gtaaggggct caccatttcc aaatctaaag tggagtcagg 469 Leu Ser Val Thr Thr 15 gcctgaggtg acaaagatat cacctttggc tttccacag gt gtc cac tcc cag gtt 525 Gly Val His Ser Gln Val 20 cag ttg cag cag tct gac gct gag ttg gtg aaa cct ggg gct tca gtg 573 Gln Leu Gln Gln Ser Asp Ala Glu Leu Val Lys Pro Gly Ala Ser Val 25 30 35 aag att tcc tgc aag gct tct ggc tac acc ttc act gac cat gct att 621 Lys Ile Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Asp His Ala Ile 40 45 50 cac tgg gtg aag cag aag cct gaa cag ggc ctg gaa tgg att gga tat 669 His Trp Val Lys Gln Lys Pro Glu Gln Gly Leu Glu Trp Ile Gly Tyr 55 60 65 att tct ccc gga aat gat gat att aag tac aat gag aag ttc aag ggc 717 Ile Ser Pro Gly Asn Asp Asp Ile Lys Tyr Asn Glu Lys Phe Lys Gly 70 75 80 85 aag gcc aca ctg act gca gac aaa tcc tcc agt act gcc tac atg caa 765 Lys Ala Thr Leu Thr Ala Asp Lys Ser Ser Ser Thr Ala Tyr Met Gln 90 95 100 ctc aac agc ctg aca tct gag gat tct gca gtg tat ttc tgt aga aga 813 Leu Asn Ser Leu Thr Ser Glu Asp Ser Ala Val Thr Phe Cys Arg Arg 105 110 115 tcc ttc tac ggc aac tgg ggc caa ggc acc acc ctc aca gtc tcc tca g 862 Ser Phe Tyr Gly Asn Trp Gly Gln Gly Thr Thr Leu Thr Val Ser Ser 120 125 130 44 430 DNA Mus musculus CC92 VH coding sequence 28..429 44 ctatccctgg acacactgac tctaacc atg gaa tgg agc tgg gtc ttt ctc ttc 54 Met Glu Trp Ser Trp Val Phe Leu Phe 1 5 ttc ctg tca gta act aca ggt gtc cac tcc cag gtt caa ctg cag cag 102 Phe Leu Ser Val Thr Thr Gly Val His Ser Gln Val Gln Leu Gln Gln 10 15 20 25 tct gac gct gag ttg gtg aaa cct ggg gct tca gtg aag ata tcc tgc 150 Ser Asp Ala Glu Leu Val Lys Pro Gly Ala Ser Val Lys Ile Ser Cys 30 35 40 aag gct tct ggc tac acc ttc act gac cat gct att cac tgg gtg aag 198 Lys Ala Ser Gly Tyr Thr Phe Thr Asp His Ala Ile His Trp Val Lys 45 50 55 cag aaa cct gaa cag ggc ctg gaa tgg att gga tat att tct ccc gga 246 Gln Lys Pro Glu Gln Gly Leu Glu Trp Ile Gly Tyr Ile Ser Pro Gly 60 65 70 aat gat gat att aag tac aat gag aag ttc aag ggt aag gcc aca ctg 294 Asn Asp Asp Ile Lys Tyr Asn Glu Lys Phe Lys Gly Lys Ala Thr Leu 75 80 85 act gca gac aaa ccc tcc aac act gtc tac atg cag ctc aac agc ctg 342 Thr Ala Asp Lys Pro Ser Asn Thr Val Tyr Met Gln Leu Asn Ser Leu 90 95 100 105 acc tct gag gat tct gca gtg tat ttc tgt aca aga tct cta tcc ggg 390 Thr Ser Glu Asp Ser Ala Val Thr Phe Cys Thr Arg Ser Leu Ser Gly 110 115 120 aac tcc tgg ggc cag ggc acc act ctc aca gtc tcc tca g 430 Asn Ser Trp Gly Gln Gly Thr Thr Leu Thr Val Ser Ser 125 130 45 117 PRT Mus musculus VHaTAG 1..117 45 Met Glu Trp Ser Trp Val Phe Leu Phe Phe Leu Ser Val Thr Thr Gly 1 5 10 15 Val His Ser Gln Val Gln Leu Gln Gln Ser Asp Ala Glu Leu Val Lys 20 25 30 Pro Gly Ala Ser Val Lys Ile Ser Cys Lys Ala Ser Gly Tyr Thr Phe 35 40 45 Thr Asp His Ala Ile His Trp Val Lys Gln Lys Pro Glu Gln Gly Leu 50 55 60 Glu Trp Ile Gly Tyr Ile Ser Pro Gly Asn Gly Asp Ile Lys Tyr Asn 65 70 75 80 Glu Lys Phe Lys Gly Lys Ala Thr Leu Thr Ala Asp Lys Ser Ser Ser 85 90 95 Thr Ala Tyr Met Gln Leu Asn Ser Leu Thr Ser Glu Asp Ser Ala Val 100 105 110 Thr Phe Cys Lys Arg 115 46 134 PRT Mus musculus CC46 VH 1..134 46 Met Glu Trp Ser Trp Val Phe Leu Phe Phe Leu Ser Val Thr Thr Gly 1 5 10 15 Val His Ser Gln Phe Gln Leu Gln Gln Ser Asp Ala Glu Leu Val Arg 20 25 30 Pro Gly Ala Ser Val Lys Ile Ser Cys Lys Ala Ser Gly Tyr Thr Phe 35 40 45 Thr Asp His Ala Ile His Trp Val Lys Gln Lys Pro Glu Gln Gly Leu 50 55 60 Glu Trp Ile Gly Tyr Phe Ser Pro Gly Asn Gly Asp Ile Lys Tyr Asn 65 70 75 80 Glu Lys Phe Lys Gly Lys Ala Thr Leu Thr Ala Asp Lys Ser Ser Ser 85 90 95 Thr Ala Tyr Met Gln Phe Asn Ser Leu Thr Ser Glu Asp Ser Ala Val 100 105 110 Thr Phe Cys Thr Gly Gly Tyr Gly Phe Ala Phe Trp Gly Gln Gly Thr 115 120 125 Leu Val Thr Val Ser Ala 130 47 134 PRT Mus musculus CC49 VH 1..134 47 Met Glu Trp Ser Trp Val Phe Leu Phe Phe Leu Ser Val Thr Thr Gly 1 5 10 15 Val His Ser Gln Val Gln Leu Gln Gln Ser Asp Ala Glu Leu Val Lys 20 25 30 Pro Gly Ala Ser Val Lys Ile Ser Cys Lys Ala Ser Gly Tyr Thr Phe 35 40 45 Thr Asp His Ala Ile His Trp Val Lys Gln Asn Pro Glu Gln Gly Leu 50 55 60 Glu Trp Ile Gly Tyr Phe Ser Pro Gly Asn Asp Asp Phe Lys Tyr Asn 65 70 75 80 Glu Arg Phe Lys Gly Lys Ala Thr Leu Thr Ala Asp Lys Ser Ser Ser 85 90 95 Thr Ala Tyr Val Gln Leu Asn Ser Leu Thr Ser Glu Asp Ser Ala Val 100 105 110 Thr Phe Cys Thr Arg Ser Leu Asn Met Ala Tyr Trp Gly Gln Gly Thr 115 120 125 Ser Val Thr Val Ser Ser 130 48 133 PRT Mus musculus CC83 VH 1..133 48 Met Glu Trp Ser Trp Val Phe Leu Phe Phe Leu Ser Val Thr Thr Gly 1 5 10 15 Val His Ser Gln Val Gln Leu Gln Gln Ser Asp Ala Glu Leu Val Lys 20 25 30 Pro Gly Ala Ser Val Lys Ile Ser Cys Lys Ala Ser Gly Tyr Thr Phe 35 40 45 Thr Asp His Ala Ile His Trp Val Lys Gln Lys Pro Glu Gln Gly Leu 50 55 60 Glu Trp Ile Gly Tyr Ile Ser Pro Gly Asn Asp Asp Ile Lys Tyr Asn 65 70 75 80 Glu Lys Phe Lys Gly Lys Ala Thr Leu Thr Ala Asp Lys Ser Ser Ser 85 90 95 Thr Ala Tyr Met Gln Leu Asn Ser Leu Thr Ser Glu Asp Ser Ala Val 100 105 110 Thr Phe Cys Arg Arg Ser Phe Tyr Gly Asn Trp Gly Gln Gly Thr Thr 115 120 125 Leu Thr Val Ser Ser 130 49 134 PRT Mus musculus CC92 VH 1..134 49 Met Glu Trp Ser Trp Val Phe Leu Phe Phe Leu Ser Val Thr Thr Gly 1 5 10 15 Val His Ser Gln Val Gln Leu Gln Gln Ser Asp Ala Glu Leu Val Lys 20 25 30 Pro Gly Ala Ser Val Lys Ile Ser Cys Lys Ala Ser Gly Tyr Thr Phe 35 40 45 Thr Asp His Ala Ile His Trp Val Lys Gln Lys Pro Glu Gln Gly Leu 50 55 60 Glu Trp Ile Gly Tyr Ile Ser Pro Gly Asn Asp Asp Ile Lys Tyr Asn 65 70 75 80 Glu Lys Phe Lys Gly Lys Ala Thr Leu Thr Ala Asp Lys Pro Ser Asn 85 90 95 Thr Val Tyr Met Gln Leu Asn Ser Leu Thr Ser Glu Asp Ser Ala Val 100 105 110 Thr Phe Cys Thr Arg Ser Leu Ser Gly Asn Ser Trp Gly Gln Gly Thr 115 120 125 Thr Leu Thr Val Ser Ser 130 50 423 DNA Artificial Sequence B17X2 VH coding sequence 7..423 DNA encoding the heavy chain variable region of the anti-N-acetylglucosamine antibody, B17X2 50 gaattc atg gaa aaa ctt tgg ttc ttg ctt ctg ctg ctg acc atc cct 48 Met Glu Lys Leu Trp Phe Leu Leu Leu Leu Leu Thr Ile Pro 1 5 10 tca tgg gtc ttg tcc cag atc acc ttg aag gag tct ggt cct acn ctg 96 Ser Trp Val Leu Ser Gln Ile Thr Leu Lys Glu Ser Gly Pro Thr Leu 15 20 25 30 gtg aaa ccc aca cag acc ctc acg ctg acc tgc acc ttc tct ggg ttc 144 Val Lys Pro Thr Gln Thr Leu Thr Leu Thr Cys Thr Phe Ser Gly Phe 35 40 45 tca ctc agc act cat gga gtg ggt gtg ggc tgg atc cgt nnn nnc cca 192 Ser Leu Ser Thr His Gly Val Gly Val Gly Trp Ile Arg Xaa Xaa Pro 50 55 60 gga aag gcc ctg gag tgg ctt gca ctc att tat tgg gat gat gat aag 240 Gly Lys Ala Leu Glu Trp Leu Ala Leu Ile Tyr Trp Asp Asp Asp Lys 65 70 75 cgc tac agc cca tct ctg aag agc agg ctc acc atc acc aag gac acc 288 Arg Tyr Ser Pro Ser Leu Lys Ser Arg Leu Thr Ile Thr Lys Asp Thr 80 85 90 tcc aaa aac cag gtg atc ctt aca atg acc aac atg gac cct gtg gac 336 Ser Lys Asn Gln Val Ile Leu Thr Met Thr Asn Met Asp Pro Val Asp 95 100 105 110 aca gcc aca tat tat tgt gca cac ggg ctg cca tct atg gtt aag aac 384 Thr Ala Thr Tyr Tyr Cys Ala His Gly Leu Pro Ser Met Val Lys Asn 115 120 125 tgg ggc caa ggg acc acg gtc acc gtc tcc tca ggg agt 423 Trp Gly Gln Gly Thr Thr Val Thr Val Ser Ser Gly Ser 130 135 51 139 PRT Artificial Sequence B17X2 VH 1..139 Heavy chain variable region of the anti-N-acetylglucosamine antibody, B17X2 51 Met Glu Lys Leu Trp Phe Leu Leu Leu Leu Leu Thr Ile Pro Ser Trp 1 5 10 15 Val Leu Ser Gln Ile Thr Leu Lys Glu Ser Gly Pro Thr Leu Val Lys 20 25 30 Pro Thr Gln Thr Leu Thr Leu Thr Cys Thr Phe Ser Gly Phe Ser Leu 35 40 45 Ser Thr His Gly Val Gly Val Gly Trp Ile Arg Xaa Xaa Pro Gly Lys 50 55 60 Ala Leu Glu Trp Leu Ala Leu Ile Tyr Trp Asp Asp Asp Lys Arg Tyr 65 70 75 80 Ser Pro Ser Leu Lys Ser Arg Leu Thr Ile Thr Lys Asp Thr Ser Lys 85 90 95 Asn Gln Val Ile Leu Thr Met Thr Asn Met Asp Pro Val Asp Thr Ala 100 105 110 Thr Tyr Tyr Cys Ala His Gly Leu Pro Ser Met Val Lys Asn Trp Gly 115 120 125 Gln Gly Thr Thr Val Thr Val Ser Ser Gly Ser 130 135 52 1954 DNA Mus musculus J-H germline genes 1..1954 Murine J-H germline genes obtained from pNP9 52 ggatcctggc cagcattgcc gctaggtccc tctcttctat gctttctttg tccctcactg 60 gcctccatct gagataatcc tggagcccta gccaaggatc atttattgtc aggggtctaa 120 tcattgttgt cacaatgtgc ctggtttgct tactggggcc aagggactct ggtcactgtc 180 tctgcaggtg agtcctaact tctcccattc taaatgcatg ttggggggat tctgagcctt 240 caggaccaag attctctgca aacgggaatc aagattcaac ccctttgtcc caaagttgag 300 acatgggtct gggtcaggga ctctctgcct gctggtctgt ggtgacatta gaactgaagt 360 atgatgaagg atctgccaga actgaagctt gaagtctgag gcagaatctt gtccagggtc 420 tatcggactc ttgtgagaat taggggctga cagttgatgg tgacaatttc agggtcagtg 480 actgtcaggt ttctctgagg tgaggctgga atataggtca ccttgaagac taaagagggg 540 tccaggggct tttctgcaca ggcagggaac agaatgtgga acaatgactt gaatggttga 600 ttcttgtgtg acaccaagaa ttggcataat gtctgagttg cccaagggtg atcttagcta 660 aaaacccact attgtgatta ctatgctatg gactactggg gtcaaggaac ctcagtcacc 720 gtctcctcag gtaagaatgg cctctccagg tctttatttt taacgtttgt tatggagttt 780 tctgagcatt gcagactaat cttggatatt tgccctgagg gagccggctg agagaagttg 840 ggaaataaat ctgtctaggg atctcagagc ctttaggaca gattatctcc acatctttga 900 aaaactaaga atctgtgtga tggtgttggt ggagtccctg gatgatggga tagggacttt 960 ggaggctcat ttgagggaga tgctaaaaca atcctatggc tggagggata gttggggctg 1020 tagttggaga ttttcagttt ttagaatgaa gtattagctg caatacttca aggaccacct 1080 ctgtgacaac cattttatac agtatccagg catagggaca aaaagtggag tggggcactt 1140 tctttagatt tgtgaggaat gttccacact agattgttta aaacttcatt tgttggaagg 1200 agctgtctta gtgattgagt caagggagaa aggcatctag cctcggtctc aaaagggtag 1260 ttgctgtcta gagaggtctg gtggagcctg caaaagtcca gctttcaaag gaacacagaa 1320 gtatgtgtat ggaatattag aagatgttgc ttttactctt aagttggttc ctaggaaaaa 1380 tagttaaata ctgtgacttt aaaatgtgag agggttttca agtactcatt tttttaaatg 1440 tccaaaattt ttgtcaatca atttgaggtc ttgtttgtgt agaactgaca ttacttaaag 1500 tttaaccgag gaatgggagt gaggctctct cataccctat tcagaactga cttttaacaa 1560 taataaatta agtttaaaat atttttaaat gaattgagca atgttgagtt gagtcaagat 1620 ggccgatcag aaccggaaca cctgcagcag ctggcaggaa gcaggtcatg tggcaaggct 1680 atttggggaa gggaaaataa aaccactagg taaacttgta gctgtggttt gaagaagtgg 1740 ttttgaaaca ctctgtccag ccccaccaaa ccgaaagtcc aggctgagca aaacaccacc 1800 tgggtaattt gcatttctaa aataagttga ggattcagcc gaaactggag aggtcctctt 1860 ttaacttatt gagttcaacc ttttaatttt agcttgagta gttctagttt ccccaaactt 1920 aagtttatcg acttctaaaa tgtatttaga attc 1954 53 26 DNA Artificial Sequence HUMVL(+) 1..26 Forward oligonucleotide primer for amplification of the Hum4 VL germline gene 53 gaagagtatc gataaaattt attgag 26 54 98 DNA Artificial Sequence HUMVL(-) 1..98 Reverse oligonucleotide primer for amplification of the Hum4 VL germline gene 54 cattaagctt agaaaagtgt acttacgttt gatcaccacc ttggtccctc cgccgaaagt 60 gagaggataa ctataatatt gctgacagta ataaactg 98 55 55 DNA Homo sapiens HJ4 coding sequence 1..55 Human J4 DNA sequence 55 ctc act ttc ggc gga ggg acc aag gtg gag atc aaa cgt aagtgcactt 49 Leu Thr Phe Gly Gly Gly Thr Lys Val Glu Ile Lys Arg 1 5 10 tcctaa 55 56 13 PRT Artificial Sequence HJ4 1..13 Human J4 region 56 Leu Thr Phe Gly Gly Gly Thr Lys Val Glu Ile Lys Arg 1 5 10 57 1088 DNA Homo sapiens Hum4 VL coding sequence part 1 448..495 Intron 496..715 Hum4 VL coding sequence part 2 716..1068 57 atcgataaaa tttattgaga atttgtttat tatgattaac agaggtaaaa gccagtatat 60 tactgattaa tataggtaaa aggcagttaa gaaattggga atgctttctc ttctgctttc 120 ttctacgatg cacaaggcgt ttcacattta tgcccctatg aaaattacta ggctgtccta 180 gtcattagat ctttcagcag tttgtagttt tagagcttct aagttgactt ctgtcttttc 240 tattcataca attacacatt ctgtgatgat atttttggct cttgatttac attgggtact 300 ttcacaaccc actgctcatg aaatttgctt ttgtactact ggttgttttt gcataggccc 360 ctccaggcca cgaccaggtg tttggatttt ataaacgggc cgtttgcatt gtgaactgag 420 ctacaacagg caggcagggg cagcaag atg gtg ttg cag acc cag gtc ttc att 474 Met Val Leu Gln Thr Gln Val Phe Ile 1 5 tct ctg ttg ctc tgg atc tct g gtgaggaatt aaaaagtgcc acagtctttt 526 Ser Leu Leu Leu Trp Ile Ser 10 15 cagagtaata tctgtgtaga aataaaaaaa attaagatat agttggaaat aatgactatt 586 tccaatatgg atccaattat ctgctgactt ataatactac tagaaagcaa atttaaatga 646 catatttcaa ttatatctga gacagcgtgt ataagtttat gtataatcat tgtccattac 706 tgactacag gt ccc tac ggg gac atc gtg atg acc cag tct cca gac tcc 756 Gly Ala Tyr Gly Asp Ile Val Met Thr Gln Ser Pro Asp Ser 20 25 30 ctg gct gtg tct ctg ggc gag agg gcc acc atc aac tgc aag tcc agc 804 Leu Ala Val Ser Leu Gly Glu Arg Ala Thr Ile Asn Cys Lys Ser Ser 35 40 45 cag agt gtt tta tac agc tcc aac aat aag aac tac tta gct tgg tac 852 Gln Ser Val Leu Tyr Ser Ser Asn Asn Lys Asn Tyr Leu Ala Trp Tyr 50 55 60 cag cag aaa cca gga cag cct cct aag ctg ctc att tac tgg gca tct 900 Gln Gln Lys Pro Gly Gln Pro Pro Lys Leu Leu Ile Tyr Trp Ala Ser 65 70 75 acc cgg gaa tcc ggg gtc cct gac cga ttc agt ggc agt ggg tct ggg 948 Thr Arg Glu Ser Gly Val Pro Asp Arg Phe Ser Gly Ser Gly Ser Gly 80 85 90 aca gat ttc act ctc acc atc agc agc ctg cag gct gaa gat gtg gca 996 Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln Ala Glu Asp Val Ala 95 100 105 110 gtt tat tac tgt cag caa tat tat agt tat cct ctc act ttc ggc gga 1044 Val Tyr Tyr Cys Gln Gln Tyr Tyr Ser Tyr Pro Leu Thr Phe Gly Gly 115 120 125 ggg acc aag gtg gtg aaa aaa cgt aagtacactt ttctaagctt 1088 Gly Thr Lys Val Val Ile Lys Arg 130 58 134 PRT Homo sapiens Hum4 VL 1..134 58 Met Val Leu Gln Thr Gln Val Phe Ile Ser Leu Leu Leu Trp Ile Ser 1 5 10 15 Gly Ala Tyr Gly Asp Ile Val Met Thr Gln Ser Pro Asp Ser Leu Ala 20 25 30 Val Ser Leu Gly Glu Arg Ala Thr Ile Asn Cys Lys Ser Ser Gln Ser 35 40 45 Val Leu Tyr Ser Ser Asn Asn Lys Asn Tyr Leu Ala Trp Tyr Gln Gln 50 55 60 Lys Pro Gly Gln Pro Pro Lys Leu Leu Ile Tyr Trp Ala Ser Thr Arg 65 70 75 80 Glu Ser Gly Val Pro Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp 85 90 95 Phe Thr Leu Thr Ile Ser Ser Leu Gln Ala Glu Asp Val Ala Val Tyr 100 105 110 Tyr Cys Gln Gln Tyr Tyr Ser Tyr Pro Leu Thr Phe Gly Gly Gly Thr 115 120 125 Lys Val Val Ile Lys Arg 130 59 40 DNA Artificial Sequence Primer CH(+) 1..40 Forward oligonucleotide primer for inserting DNA encoding a polylinker into plasmid pSV2neo-101 to create plasmid pSV2neo-102 59 ggatcatcga ttgatatcaa ctagtgaagc ttatggatcc 40 60 40 DNA Artificial Sequence Primer CH(-) 1..40 Reverse oligonucleotide primer for inserting DNA encoding a polylinker into plasmid pSV2neo-101 to create plasmid pSV2neo-102 60 ggatccataa gcttcactag ttgatatcaa tcgatgatcc 40 61 93 DNA Artificial Sequence Primer NEO102SEQ 4..24 Primer for sequencing plasmids pSV2neo-102 and pRL1000 61 gaggaggtta gggtttatga ggacacagag gagcttcctg gggatccaga catgataaga 60 tacattgatg agtttggaca aaccacaact aga 93 62 258 DNA Artificial Sequence Oligonucleotide Hind IIIC?(-) complementary sequence 53..80 DNA sequence complementary to the oligonucleotide primer, Hind IIICk(-), used to sequence versions of plasmid pRL1000 containing the Hum4 VL coding sequence 62 cttcctgggg atcatcgatt gatatcaact agttgaagct tttttttttt cagtgctatt 60 taattatttc aatatcctct catcaaatgt atttaaataa caaaagctca accaaaaaga 120 aagaaatatg taattctttc agagtaaaaa tcacacccat gacctggcca ctgagggctt 180 gatcaattca ctttgaattt ggcattaaat accattaagg tatattaact gattttaaaa 240 taagatatat tcgtgacc 258 63 836 DNA Artificial Sequence SCFV1 coding sequence 20..799 DNA sequence encoding the single chain anti-TAG-72 antibody SCFV1 63 aaaaactata agctccatg atg ctt ttg caa gct ttc ctt ttc ctt ttg gct 52 Met Leu Leu Gln Ala Phe Leu Phe Leu Leu Ala 1 5 10 ggt ttt gca gcc aaa ata tct gca gac atc gtg atg acc cag tct cca 100 Gly Phe Ala Ala Lys Ile Ser Ala Asp Ile Val Met Thr Gln Ser Pro 15 20 25 gac tcc ctg gct gtg tct ctg ggc gag agg gcc acc atc aac tgc aag 148 Asp Ser Leu Ala Val Ser Leu Gly Glu Arg Ala Thr Ile Asn Cys Lys 30 35 40 tcc agc tgc aag gtt tta tac agc tcc aac aat aag aac tac tta gct 196 Ser Ser Gln Ser Val Leu Tyr Ser Ser Asn Asn Lys Asn Tyr Leu Ala 45 50 55 tgg tac cag cag aaa cca gga cag cct cct aag ctg ctc att tac tgg 244 Trp Tyr Gln Gln Lys Pro Gly Gln Pro Pro Lys Leu Leu Ile Tyr Trp 60 65 70 75 gca tct acc cgg gaa tcc ggg gtc cct gac cga ttc agt ggc agc ggg 292 Ala Ser Thr Arg Glu Ser Gly Val Pro Asp Arg Phe Ser Gly Ser Gly 80 85 90 tct ggg aca gat ttc act ctc acc atc agc agc ctg cag gct gaa gat 340 Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln Ala Glu Asp 95 100 105 gtg gca gtt tat tac tgt cag caa tat tat agt tat cct ctc act ttc 388 Val Ala Val Tyr Tyr Cys Gln Gln Tyr Tyr Ser Tyr Pro Leu Thr Phe 110 115 120 ggc gga ggg acc aag gtg aag gag tca ggt tcg gtc tcc tca gaa caa 436 Gly Gly Gly Thr Lys Val Lys Glu Ser Gly Ser Val Ser Ser Glu Gln 125 130 135 ttg gcc caa ttt cgt tcc tta gac gtc cag ttg cag cag tct gac gct 484 Leu Ala Gln Phe Arg Ser Leu Asp Val Gln Leu Gln Gln Ser Asp Ala 140 145 150 155 gag ttg gtg aaa cct ggg gct tca gtg aag aat tcc tgc aag gct tct 532 Glu Leu Val Lys Pro Gly Ala Ser Val Lys Ile Ser Cys Lys Ala Ser 160 165 170 ggc tac acc ttc act gac cat gca att cac tgg gtg aaa cag aac cct 580 Gly Tyr Thr Phe Thr Asp His Ala Ile His Trp Val Lys Gln Asn Pro 175 180 185 gaa cag ggc ctg gaa tgg att gga tat ttt tct ccc gga aat gat gat 628 Glu Gln Gly Leu Glu Trp Ile Gly Tyr Phe Ser Pro Gly Asn Asp Asp 190 195 200 ttt aaa tac aat gag agg ttc aag ggc aag gcc aca ctg act gca gac 676 Phe Lys Tyr Asn Glu Arg Phe Lys Gly Lys Ala Thr Leu Thr Ala Asp 205 210 215 aaa tcc tcc agc act gcc tac gtg cag ctc aac agc ctg aca tct gag 724 Lys Ser Ser Ser Thr Ala Tyr Val Gln Leu Asn Ser Leu Thr Ser Glu 220 225 230 235 gat tct gca gtg tat ttc tgt aca aga tcc ctg aat atg gcc tac tgg 772 Asp Ser Ala Val Tyr Phe Cys Thr Arg Ser Leu Asn Met Ala Tyr Trp 240 245 250 ggt caa gga acc tca gtc acc gtc tcc tagtgaagct tggaacacca 819 Gly Gln Gly Thr Ser Val Thr Val Ser 255 260 cacaaaccat atccaaa 836 64 260 PRT Artificial Sequence SCFV1 1..260 Single chain anti-TAG-72 antibody, SCFV1 64 Met Leu Leu Gln Ala Phe Leu Phe Leu Leu Ala Gly Phe Ala Ala Lys 1 5 10 15 Ile Ser Ala Asp Ile Val Met Thr Gln Ser Pro Asp Ser Leu Ala Val 20 25 30 Ser Leu Gly Glu Arg Ala Thr Ile Asn Cys Lys Ser Ser Gln Ser Val 35 40 45 Leu Tyr Ser Ser Asn Asn Lys Asn Tyr Leu Ala Trp Tyr Gln Gln Lys 50 55 60 Pro Gly Gln Pro Pro Lys Leu Leu Ile Tyr Trp Ala Ser Thr Arg Glu 65 70 75 80 Ser Gly Val Pro Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe 85 90 95 Thr Leu Thr Ile Ser Ser Leu Gln Ala Glu Asp Val Ala Val Tyr Tyr 100 105 110 Cys Gln Gln Tyr Tyr Ser Tyr Pro Leu Thr Phe Gly Gly Gly Thr Lys 115 120 125 Val Lys Glu Ser Gly Ser Val Ser Ser Glu Gln Leu Ala Gln Phe Arg 130 135 140 Ser Leu Asp Val Gln Leu Gln Gln Ser Asp Ala Glu Leu Val Lys Pro 145 150 155 160 Gly Ala Ser Val Lys Ile Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr 165 170 175 Asp His Ala Ile His Trp Val Lys Gln Asn Pro Glu Gln Gly Leu Glu 180 185 190 Trp Ile Gly Tyr Phe Ser Pro Gly Asn Asp Asp Phe Lys Tyr Asn Glu 195 200 205 Arg Phe Lys Gly Lys Ala Thr Leu Thr Ala Asp Lys Ser Ser Ser Thr 210 215 220 Ala Tyr Val Gln Leu Asn Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr 225 230 235 240 Phe Cys Thr Arg Ser Leu Asn Met Ala Tyr Trp Gly Gln Gly Thr Ser 245 250 255 Val Thr Val Ser 260 65 1330 DNA Artificial Sequence pSCFVUHH SCFV coding sequence 293..1114 DNA in plasmid pSCFVUHH, encoding a Hum4 VL-linker-CC49 VH single chain anti-TAG-72 antibody 65 ctcatgtttg acagcttatc atcgatgaat tccatcactt ccctccgttc atttgtcccc 60 ggtggaaacg aggtcatcat ttccttccga aaaaacggtt gcatttaaat cttacatatg 120 taatactttc aaagactaca tttgtaagat ttgatgtttg agtcggctga aagatcgtac 180 gtaccaatta ttgtttcgtg attgttcaag ccataacact gtagggatag tggaaagagt 240 gcttcatctg gttacgatca atcaaatatt caaacggagg gagacgattt tg atg aaa 298 Met Lys 1 tac cta ttg cct acg gca gcc gct gga ttg tta tta ctc gct gcc caa 346 Tyr Leu Leu Pro Thr Ala Ala Ala Gly Leu Leu Leu Leu Ala Ala Gln 5 10 15 cca gcc atg gcc gac atc gtg atg acc cag tct cca gac tcc ctg gct 394 Pro Ala Met Ala Asp Ile Val Met Thr Gln Ser Pro Asp Ser Leu Ala 20 25 30 gtg tct ctg ggc gag agg gcc acc atc aac tgc aag tcc agc cag agt 442 Val Ser Leu Gly Glu Arg Ala Thr Ile Asn Cys Lys Ser Ser Gln Ser 35 40 45 50 gtt tta tac agc tcc aac aat aag aac tac tta gct tgg tac cag cag 490 Val Leu Tyr Ser Ser Asn Asn Lys Asn Tyr Leu Ala Trp Tyr Gln Gln 55 60 65 aaa cca gga cag cct cct aag ctg ctc att tac tgg gca tct acc cgg 538 Lys Pro Gly Gln Pro Pro Lys Leu Leu Ile Tyr Trp Ala Ser Thr Arg 70 75 80 gaa tcc ggg gtc cct gac cga ttc agt ggc agc ggg tct ggg aca gat 586 Glu Ser Gly Val Pro Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp 85 90 95 ttc act ctc acc atc agc agc ctg cag gct gaa gat gtg gca gtt tat 634 Phe Thr Leu Thr Ile Ser Ser Leu Gln Ala Glu Asp Val Ala Val Tyr 100 105 110 tac tgt cag caa tat tat agt tat cct ctc act ttc ggc gga ggg acc 682 Tyr Cys Gln Gln Tyr Tyr Ser Tyr Pro Leu Thr Phe Gly Gly Gly Thr 115 120 125 130 aag gtg gtg atc aag ctt agt gcg gac gat gcg aaa aag gat gct gcg 730 Lys Val Val Ile Lys Leu Ser Ala Asp Asp Ala Lys Lys Asp Ala Ala 135 140 145 aag aag gat gac gct aag aaa gac gat gct aaa aag gac ctc cag gtt 778 Lys Lys Asp Asp Ala Lys Lys Asp Asp Ala Lys Lys Asp Leu Gln Val 150 155 160 cag ttg cag cag tct gct gag ttg gtg aaa cct ggg gct tca gtg aag 826 Gln Leu Gln Gln Ser Ala Glu Leu Val Lys Pro Gly Ala Ser Val Lys 165 170 175 att tcc tgc aag gct tct ggc tac acc ttc act gac cat gca att cac 874 Ile Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Asp His Ala Ile His 180 185 190 tgg gtg aaa cag aac cct gaa cag ggc ctg gaa tgg att gga tat ttt 922 Trp Val Lys Gln Asn Pro Glu Gln Gly Leu Glu Trp Ile Gly Tyr Phe 195 200 205 210 tct ccc gga aat gat gat ttt aaa tac aat gag agg ttc aag ggc aag 970 Ser Pro Gly Asn Asp Asp Phe Lys Tyr Asn Glu Arg Phe Lys Gly Lys 215 220 225 gcc aca ctg act gca gac aaa tcc tcc agc act gcc tac gtg cag ctc 1018 Ala Thr Leu Thr Ala Asp Lys Ser Ser Ser Thr Ala Tyr Val Gln Leu 230 235 240 aac agc ctg aca tct gag gat tct gca gtg tat ttc tct aca aga tcc 1066 Asn Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Phe Cys Thr Arg Ser 245 250 255 ctg aat atg gcc tac tgg ggt caa gga acc tca gtc acc gtc tcc tca 1114 Leu Asn Met Ala Tyr Trp Gly Gln Gly Thr Ser Val Thr Val Ser Ser 260 265 270 taaaaagcta gcgatgaatc cgtcaaaaca tcatcttaca taaagtcact tggtgatcaa 1174 gctcatatca ttgtccggca atggtgtggg ctttttttgt tttctatctt taaagatcat 1234 gtgaaggaaa aaacgggaaa atcggtctgc gggaaaggac cgggtttttg tcgaaatcat 1294 aggcgaatgg gttggattgt gacaaaattc ggatcc 1330 66 274 PRT Artificial Sequence pSCFVUHH SCFV 1..274 The Hum4 VL-linker-CC49 VH single chain anti-TAG-72 antibody expressed from plasmid pSCFVUHH 66 Met Lys Tyr Leu Leu Pro Thr Ala Ala Ala Gly Leu Leu Leu Leu Ala 1 5 10 15 Ala Gln Pro Ala Met Ala Asp Ile Val Met Thr Gln Ser Pro Asp Ser 20 25 30 Leu Ala Val Ser Leu Gly Glu Arg Ala Thr Ile Asn Cys Lys Ser Ser 35 40 45 Gln Ser Val Leu Tyr Ser Ser Asn Asn Lys Asn Tyr Leu Ala Trp Tyr 50 55 60 Gln Gln Lys Pro Gly Gln Pro Pro Lys Leu Leu Ile Tyr Trp Ala Ser 65 70 75 80 Thr Arg Glu Ser Gly Val Pro Asp Arg Phe Ser Gly Ser Gly Ser Gly 85 90 95 Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln Ala Glu Asp Val Ala 100 105 110 Val Tyr Tyr Cys Gln Gln Tyr Tyr Ser Tyr Pro Leu Thr Phe Gly Gly 115 120 125 Gly Thr Lys Val Val Ile Lys Leu Ser Ala Asp Asp Ala Lys Lys Asp 130 135 140 Ala Ala Lys Lys Asp Asp Ala Lys Lys Asp Asp Ala Lys Lys Asp Leu 145 150 155 160 Gln Val Gln Leu Gln Gln Ser Ala Glu Leu Val Lys Pro Gly Ala Ser 165 170 175 Val Lys Ile Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Asp His Ala 180 185 190 Ile His Trp Val Lys Gln Asn Pro Glu Gln Gly Leu Glu Trp Ile Gly 195 200 205 Tyr Phe Ser Pro Gly Asn Asp Asp Phe Lys Tyr Asn Glu Arg Phe Lys 210 215 220 Gly Lys Ala Thr Leu Thr Ala Asp Lys Ser Ser Ser Thr Ala Tyr Val 225 230 235 240 Gln Leu Asn Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Phe Cys Thr 245 250 255 Arg Ser Leu Asn Met Ala Tyr Trp Gly Gln Gly Thr Ser Val Thr Val 260 265 270 Ser Ser 67 1028 DNA Artificial Sequence FLAG peptide coding sequence 784..813 DNA in plasmid pATDFLAG, encoding the FLAG peptide adapter 67 ctcatgtttg acagcttatc atcgatgaat tccatcactt ccctccgttc atttgtcccc 60 ggtggaaacg aggtcatcat ttccttccga aaaaacggtt gcatttaaat cttacatatg 120 taatactttc aaagactaca tttgtaagat ttgatgtttg agtcggctga aagatcgtac 180 gtaccaatta ttgtttcgtg attgttcaag ccataacact gtagggatag tggaaagagt 240 gcttcatctg gttacgatca atcaaatatt caaacggagg gagacgattt tg atg aaa 298 Met Lys 1 tac cta ttg cct acg gca gcc gct gga ttg tta tta ctc gct gcc caa 346 Tyr Leu Leu Pro Thr Ala Ala Ala Gly Leu Leu Leu Leu Ala Ala Gln 5 10 15 cca gcc atg gcc gac atc gtg atg acc cag tct cca gac tcc ctg gct 394 Pro Ala Met Ala Asp Ile Val Met Thr Gln Ser Pro Asp Ser Leu Ala 20 25 30 gtg tct ctg ggc gag agg gcc acc atc aac tgc aag tcc agc cag agt 442 Val Ser Leu Gly Glu Arg Ala Thr Ile Asn Cys Lys Ser Ser Gln Ser 35 40 45 50 gtt tta tac agc tcc aac aat aag aac tac tta gct tgg tac cag cag 490 Val Leu Tyr Ser Ser Asn Asn Lys Asn Tyr Leu Ala Trp Tyr Gln Gln 55 60 65 aaa cca gga cag cct cct aag ctg ctc att tac tgg gca tct acc cgg 538 Lys Pro Gly Gln Pro Pro Lys Leu Leu Ile Tyr Trp Ala Ser Thr Arg 70 75 80 gaa tcc ggg gtc cct gac cga ttc agt ggc agc ggg tct ggg aca gat 586 Glu Ser Gly Val Pro Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp 85 90 95 ttc act ctc acc atc agc agc ctg cag gct gaa gat gtg gca gtt tat 634 Phe Thr Leu Thr Ile Ser Ser Leu Gln Ala Glu Asp Val Ala Val Tyr 100 105 110 tac tgt cag caa tat tat agt tat cct ctc act ttc ggc gga ggg acc 682 Tyr Cys Gln Gln Tyr Tyr Ser Tyr Pro Leu Thr Phe Gly Gly Gly Thr 115 120 125 130 aag gtg gtg atc aag ctt agt gcg gac gat gcg aaa aag gat gct gcg 730 Lys Val Val Ile Lys Leu Ser Ala Asp Asp Ala Lys Lys Asp Ala Ala 135 140 145 aag aag gat gac gct aag aaa gac gat gct aaa aag gac ctc cag 775 Lys Lys Asp Asp Ala Lys Lys Asp Asp Ala Lys Lys Asp Leu Gln 150 155 160 acaatgtc gct agc gac tac aag gac gat gat gac aaa taaaaacct 822 Ala Ser Asp Tyr Lys Asp Asp Asp Asp Lys 1 5 10 agcgatgaat ccgtcaaaac atcatcttac ataaagtcac ttggtgatca agctcatatc 882 attgtccggc aatggtgtgg gctttttttg ttttcatctt taaagatcat gtgaaggaaa 942 aaacgggaaa atcggtctgc gggaaaggac cgggtttttg tcgaaatcat aggcgaatgg 1002 gttggattgt gacaaaattc ggatcc 1028 68 10 PRT Artificial Sequence FLAG peptide 1..10 The FLAG peptide adapter 68 Ala Ser Asp Tyr Lys Asp Asp Asp Asp Lys 1 5 10 69 1359 DNA Artificial Sequence pSC49FLAG SCFV coding sequence 293..1144 DNA in plasmid pSC49FLAG, encoding a Hum4 VL-linker-CC49 VH single chain anti-TAG-72 antibody 69 ctcatgtttg acagcttatc atcgatgaat tccatcactt ccctccgttc atttgtcccc 60 ggtggaaacg aggtcatcat ttccttccga aaaaacggtt gcatttaaat cttacatatg 120 taatactttc aaagactaca tttgtaagat ttgatgtttg agtcggctga aagatcgtac 180 gtaccaatta ttgtttcgtg attgttcaag ccataacact gtagggatag tggaaagagt 240 gcttcatctg gttacgatca atcaaatatt caaacggagg gagacgattt tg atg aaa 298 Met Lys 1 tac cta ttg cct acg gca gcc gct gga ttg tta tta ctc gct gcc caa 346 Tyr Leu Leu Pro Thr Ala Ala Ala Gly Leu Leu Leu Leu Ala Ala Gln 5 10 15 cca gcc atg gcc gac atc gtg atg acc cag tct cca gac tcc ctg gct 394 Pro Ala Met Ala Asp Ile Val Met Thr Gln Ser Pro Asp Ser Leu Ala 20 25 30 gtg tct ctg ggc gag agg gcc acc atc aac tgc aag tcc agc cag agt 442 Val Ser Ser Gly Glu Arg Ala Thr Ile Asn Cys Lys Ser Ser Gln Ser 35 40 45 50 gtt tta tac agc tcc aac aat aag acc tac tta gct tgg tac cag cag 490 Val Leu Tyr Ser Ser Asn Asn Lys Asn Tyr Leu Ala Trp Tyr Gln Gln 55 60 65 aaa cca gga cag cct cct aag ctg ctc att tac tgg gca tct acc cgg 538 Lys Pro Gly Gln Pro Pro Lys Leu Leu Ile Tyr Trp Ala Ser Thr Arg 70 75 80 gaa tcc ggg gtc cct gac cga ttc agt ggc agc ggg tct ggg aca gat 586 Glu Ser Gly Val Pro Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp 85 90 95 ttc act ctc acc atc agc agc ctg cag gct gaa gat gtg gca gtt tat 634 Phe Thr Leu Thr Ile Ser Ser Leu Gln Ala Glu Asp Val Ala Val Tyr 100 105 110 tac tgt cag caa tat tat agt tat cct ctc act ttc ggc gga ggg acc 682 Tyr Cys Gln Gln Tyr Tyr Ser Tyr Pro Leu Thr Phe Gly Gly Gly Thr 115 120 125 130 aag gtg gtg atc aag ctt agt gcg gac gat gcg aaa aag gat gct gcg 730 Lys Val Val Ile Lys Leu Ser Ala Asp Asp Ala Lys Lys Asp Ala Ala 135 140 145 aag aag gat gac gct aag aaa gac gat gct aaa aag gac ctc cag gtt 778 Lys Lys Asp Asp Ala Lys Lys Asp Asp Ala Lys Lys Asp Leu Gln Val 150 155 160 cag ttg cag cag tct gct gag ttg gtg aaa cct ggg gct tca gtg aag 826 Gln Leu Gln Gln Ser Ala Glu Leu Val Lys Pro Gly Ala Ser Val Lys 165 170 175 att tcc tgc aag gct tct ggc tac acc ttc act gac cat gca att cac 874 Ile Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Asp His Ala Ile His 180 185 190 tgg gtg aaa cag aac cct gaa cag ggc ctg gaa tgg att gga tat ttt 922 Trp Val Lys Gln Asn Pro Glu Gln Gly Leu Glu Trp Ile Gly Tyr Phe 195 200 205 210 tct ccc gga aat gat gat ttt aaa tac aat gag agg ttc aag ggc aag 970 Ser Pro Gly Asn Asp Asp Phe Lys Tyr Asn Glu Arg Phe Lys Gly Lys 215 220 225 gcc aca ctg act gca gac aaa tcc tcc agc act gcc tac gtg cag ctc 1018 Ala Thr Leu Thr Ala Asp Lys Ser Ser Ser Thr Ala Tyr Val Gln Leu 230 235 240 aac agc ctg aca tct gag gat tct gca gtg tat ttc tgt aca aga tcc 1066 Asn Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Phe Cys Thr Arg Ser 245 250 255 ctg aat atg gcc tac tgg ggt caa gga acc tca gtc acc gtc tcc tca 1114 Leu Asn Met Ala Tyr Trp Gly Gln Gly Thr Ser Val Thr Val Ser Ser 260 265 270 gct agc gac tac aag gac gat gat gac aaa taaaaaccta gcgatgaatc 1164 Ala Ser Asp Tyr Lys Asp Asp Asp Asp Lys 275 280 cgtcaaaaca tcatcttaca taaagtcact tggtgatcaa gctcatatca ttgtccggca 1224 atggtgtggg ctttttttgt tttcatcttt aaagatcatg tgaaggaaaa aacgggaaaa 1284 tcggtctgcg ggaaaggacc gggtttttgt cgaaatcata ggcgaatggg ttggattgtg 1344 acaaaattcg gatcc 1359 70 284 PRT Artificial Sequence pSC49FLAG SCFV 1..284 The Hum4 VL-linker-CC49 VH single chain anti-TAG-72 antibody expressed from plasmid pSC49FLAG 70 Met Lys Tyr Leu Leu Pro Thr Ala Ala Ala Gly Leu Leu Leu Leu Ala 1 5 10 15 Ala Gln Pro Ala Met Ala Asp Ile Val Met Thr Gln Ser Pro Asp Ser 20 25 30 Leu Ala Val Ser Ser Gly Glu Arg Ala Thr Ile Asn Cys Lys Ser Ser 35 40 45 Gln Ser Val Leu Tyr Ser Ser Asn Asn Lys Asn Tyr Leu Ala Trp Tyr 50 55 60 Gln Gln Lys Pro Gly Gln Pro Pro Lys Leu Leu Ile Tyr Trp Ala Ser 65 70 75 80 Thr Arg Glu Ser Gly Val Pro Asp Arg Phe Ser Gly Ser Gly Ser Gly 85 90 95 Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln Ala Glu Asp Val Ala 100 105 110 Val Tyr Tyr Cys Gln Gln Tyr Tyr Ser Tyr Pro Leu Thr Phe Gly Gly 115 120 125 Gly Thr Lys Val Val Ile Lys Leu Ser Ala Asp Asp Ala Lys Lys Asp 130 135 140 Ala Ala Lys Lys Asp Asp Ala Lys Lys Asp Asp Ala Lys Lys Asp Leu 145 150 155 160 Gln Val Gln Leu Gln Gln Ser Ala Glu Leu Val Lys Pro Gly Ala Ser 165 170 175 Val Lys Ile Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Asp His Ala 180 185 190 Ile His Trp Val Lys Gln Asn Pro Glu Gln Gly Leu Glu Trp Ile Gly 195 200 205 Tyr Phe Ser Pro Gly Asn Asp Asp Phe Lys Tyr Asn Glu Arg Phe Lys 210 215 220 Gly Lys Ala Thr Leu Thr Ala Asp Lys Ser Ser Ser Thr Ala Tyr Val 225 230 235 240 Gln Leu Asn Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Phe Cys Thr 245 250 255 Arg Ser Leu Asn Met Ala Tyr Trp Gly Gln Gly Thr Ser Val Thr Val 260 265 270 Ser Ser Ala Ser Asp Tyr Lys Asp Asp Asp Asp Lys 275 280 71 113 PRT Mus musculus Murine CC49 VL 1..113 71 Asp Ile Val Met Ser Gln Ser Pro Ser Ser Leu Pro Val Ser Val Gly 1 5 10 15 Glu Lys Val Thr Leu Ser Cys Lys Ser Ser Gln Ser Leu Leu Tyr Ser 20 25 30 Gly Asn Gln Lys Asn Tyr Leu Ala Trp Tyr Gln Gln Lys Pro Gly Gln 35 40 45 Ser Pro Lys Leu Leu Ile Tyr Trp Ala Ser Ala Arg Glu Ser Gly Val 50 55 60 Pro Asp Arg Phe Thr Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Ser 65 70 75 80 Ile Ser Ser Val Lys Thr Glu Asp Leu Ala Val Tyr Tyr Cys Gln Gln 85 90 95 Tyr Tyr Ser Tyr Pro Leu Thr Phe Gly Ala Gly Thr Lys Leu Val Leu 100 105 110 Lys 72 113 PRT Homo sapiens LEN VL 1..113 72 Asp Ile Val Met Thr Gln Ser Pro Asp Ser Leu Ala Val Ser Leu Gly 1 5 10 15 Glu Arg Ala Thr Ile Asn Cys Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa 20 25 30 Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Trp Tyr Gln Gln Lys Pro Gly Gln 35 40 45 Pro Pro Lys Leu Leu Ile Tyr Xaa Xaa Xaa Xaa Xaa Xaa Xaa Gly Val 50 55 60 Pro Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr 65 70 75 80 Ile Ser Ser Leu Gln Ala Glu Asp Val Ala Val Tyr Tyr Cys Xaa Xaa 85 90 95 Xaa Xaa Xaa Xaa Xaa Xaa Xaa Phe Gly Gln Gly Thr Lys Leu Glu Ile 100 105 110 Lys 73 113 PRT Artificial Sequence HuCC49 VL 1..113 Humanized CC49 light chain variable region with Hum4 VL FRs 73 Asp Ile Val Met Ser Gln Ser Pro Asp Ser Leu Ala Val Ser Leu Gly 1 5 10 15 Glu Arg Val Thr Leu Asn Cys Lys Ser Ser Gln Ser Leu Leu Tyr Ser 20 25 30 Gly Asn Gln Lys Asn Tyr Leu Ala Trp Tyr Gln Gln Lys Pro Gly Gln 35 40 45 Ser Pro Lys Leu Leu Ile Tyr Trp Ala Ser Ala Arg Glu Ser Gly Val 50 55 60 Pro Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr 65 70 75 80 Ile Ser Ser Val Gln Ala Glu Asp Val Ala Val Tyr Tyr Cys Gln Gln 85 90 95 Tyr Tyr Ser Tyr Pro Leu Thr Phe Gly Ala Gly Thr Lys Leu Glu Leu 100 105 110 Lys 74 115 PRT Mus musculus Murine CC49 VH 1..115 74 Gln Val Gln Leu Gln Gln Ser Asp Ala Glu Leu Val Lys Pro Gly Ala 1 5 10 15 Ser Val Lys Ile Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Asp His 20 25 30 Ala Ile His Trp Val Lys Gln Asn Pro Glu Gln Gly Leu Glu Trp Ile 35 40 45 Gly Tyr Phe Ser Pro Gly Asn Asp Asp Phe Lys Tyr Asn Glu Arg Phe 50 55 60 Lys Gly Lys Ala Thr Leu Thr Ala Asp Lys Ser Ser Ser Thr Ala Tyr 65 70 75 80 Val Gln Leu Asn Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Phe Cys 85 90 95 Thr Arg Ser Leu Asn Met Ala Tyr Trp Gly Gln Gly Thr Ser Val Thr 100 105 110 Val Ser Ser 115 75 115 PRT Homo sapiens Human 21/28′CL VH 1..115 75 Gln Val Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys Pro Gly Ala 1 5 10 15 Ser Val Lys Val Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Xaa Xaa 20 25 30 Xaa Xaa Xaa Trp Val Arg Gln Ala Pro Gly Gln Arg Leu Glu Trp Met 35 40 45 Gly Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa 50 55 60 Xaa Xaa Arg Val Thr Ile Thr Arg Asp Thr Ser Ala Ser Thr Ala Tyr 65 70 75 80 Met Glu Leu Ser Ser Leu Arg Ser Glu Asp Thr Ala Val Tyr Tyr Cys 85 90 95 Ala Arg Xaa Xaa Xaa Xaa Xaa Xaa Trp Gly Gln Gly Thr Leu Val Thr 100 105 110 Val Ser Ser 115 76 115 PRT Artificial Sequence HuCC49 VH 1..115 Humanized CC49 heavy chain variable region with 21/28′CL VH FRs 76 Gln Val Gln Leu Val Gln Ser Gly Ala Glu Val Val Lys Pro Gly Ala 1 5 10 15 Ser Val Lys Ile Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Asp His 20 25 30 Ala Ile His Trp Val Lys Gln Asn Pro Gly Gln Arg Leu Glu Trp Ile 35 40 45 Gly Tyr Phe Ser Pro Gly Asn Asp Asp Phe Lys Tyr Asn Glu Arg Phe 50 55 60 Lys Gly Lys Ala Thr Leu Thr Ala Asp Thr Ser Ala Ser Thr Ala Tyr 65 70 75 80 Val Glu Leu Ser Ser Leu Arg Ser Glu Asp Thr Ala Val Tyr Phe Cys 85 90 95 Thr Arg Ser Leu Asn Met Ala Tyr Trp Gly Gln Gly Thr Leu Val Thr 100 105 110 Val Ser Ser 115 77 424 DNA Artificial Sequence HuCC49 VL coding sequence 14..412 DNA encoding a humanized CC49 light chain variable region with Hum4 VL FRs 77 gcaagcttcc acc atg gat agc cag gcc cag gtg ctc atg ctc ctg ctg 49 Met Asp Ser Gln Ala Gln Val Leu Met Leu Leu Leu 1 5 10 ctg tgg gtg agc ggc aca tgc ggc gac atc gtg atg agc cag tct cca 97 Leu Trp Val Ser Gly Lys Cys Gly Asp Ile Val Met Ser Gln Ser Pro 15 20 25 gac tcc ctg gcc gtg tcc ctg ggc gag agg gtg act ctg aat tgc aag 145 Asp Ser Leu Ala Val Ser Leu Gly Glu Arg Val Thr Leu Asn Cys Lys 30 35 40 tcc agc cag tcc ctg ctc tat agc gga aat cag aag aac tat ctc gcc 193 Ser Ser Gln Ser Leu Leu Tyr Ser Gly Asn Gln Lys Asn Tyr Leu Ala 45 50 55 60 tgg tat cag cag aaa cca ggg cag agc cct aaa ctg ctg att tac tgg 241 Trp Tyr Gln Gln Lys Pro Gly Gln Ser Pro Lys Leu Leu Ile Tyr Trp 65 70 75 gca tcc gct agg gaa tcc ggc gtg cct gat cgc ttc agc ggc agc gga 289 Ala Ser Ala Arg Glu Ser Gly Val Pro Asp Arg Phe Ser Gly Ser Gly 80 85 90 tct ggg aca gac ttc act ctg aca atc agc agc gtg cag gca gaa gac 337 Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Tyr Gln Ala Glu Asp 95 100 105 gtg gca gtc tat tat tgt cag cag tat tat agc tat ccc ctc aca ttc 385 Val Ala Val Tyr Tyr Cys Gln Gln Tyr Tyr Ser Tyr Pro Leu Thr Phe 110 115 120 ggc gct ggc acc aag ctg gaa ctg aaa cgggccgcgg ct 424 Gly Ala Gly Thr Lys Leu Glu Leu Lys 125 130 78 434 DNA Artificial Sequence HuCC49 VH coding sequence 14..419 DNA encoding a humanized CC49 heavy chain variable region with 21/28′CL VH FRs 78 ctaagcttcc acc atg gag tgg tcc tgg gtc ttc ctc ttc ttc ctg tcc 49 Met Glu Trp Ser Trp Val Phe Leu Phe Phe Leu Ser 1 5 10 gtg act act gga gtg cac tcc cag gtc cag ctg gtg cag tcc ggc gct 97 Val Thr Thr Gly Val His Ser Gln Val Gln Leu Val Gln Ser Gly Ala 15 20 25 gag gtg gtg aaa cct ggg gct tcc gtg aag att tcc tgc aag gca agc 145 Glu Val Val Lys Pro Gly Ala Ser Val Lys Ile Ser Cys Lys Ala Ser 30 35 40 ggc tac acc ttc act gat cac gca atc cac tgg gtg aaa cag aat cct 193 Gly Tyr Thr Phe Thr Asp His Ala Ile His Trp Val Lys Gln Asn Pro 45 50 55 60 gga cag cgc ctg gag tgg att gga tat ttc tct ccc gga aac gat gat 241 Gly Gln Gly Leu Glu Trp Ile Gly Tyr Phe Ser Pro Gly Asn Asp Asp 65 70 75 ttt aag tac aat gag agg ttc aag ggc aag gcc aca ctg act gca gac 289 Phe Lys Tyr Asn Glu Arg Phe Lys Gly Lys Ala Thr Leu Thr Ala Asp 80 85 90 aca tct gcc agc act gcc tac gtg gag ctc tcc agc ctg aga tcc gag 337 Thr Ser Ala Ser Thr Ala Tyr Val Glu Leu Ser Ser Leu Arg Ser Glu 95 100 105 gat act gca gtg tac ttc tgc aca aga tcc ctg aat atg gcc tac tgg 385 Asp Thr Ala Val Tyr Phe Cys Thr Arg Ser Leu Asn Met Ala Tyr Trp 110 115 120 gga cag gga acc ctg gtc acc gtc tcc agc gccaaaacta cgggcccat 434 Gly Gln Gly Thr Leu Val Thr Val Ser Ser 125 130 

What is claimed is:
 1. A humanized or composite anti-TAG-72 antibody or anti-TAG-72 antibody fragment which comprises a CDR-grafted light chain having light chain CDRs of a murine anti-TAG-72 antibody grafted onto a human subgroup IV kappa light chain, wherein the murine anti-TAG-72 antibody is selected from the group consisting of CC49, CC83, CC46, CC92, CC30, and CC11.
 2. The humanized or composite anti-TAG-72 antibody or anti-TAG-72 antibody fragment of claim 1 wherein said murine anti-TAG-72 antibody is CC49.
 3. The humanized or composite anti-TAG-72 antibody or anti-TAG-72 antibody fragment of claim 2 wherein the antibody or fragment comprises a heavy chain variable region whose amino acid sequence is identical to that encoded by SEQ ID NO:78 or is SEQ ID NO:76, or a light chain variable region whose amino acid sequence is identical to that encoded by SEQ ID NO:77 or is SEQ ID NO:73, or both said heavy chain variable region and said light chain variable region.
 4. The humanized or composite anti-TAG-72 antibody or anti-TAG-72 antibody fragment of claim 3 said antibody is expressed by the cell line deposited as ATCC HB-12404 and said fragment has an amino acid sequence identical to that of a constituent, TAG-72-binding part of the antibody expressed by the cell line deposited as ATCC HB-12404.
 5. A composition suitable for the treatment or in vivo or in vitro detection of cancer comprising, respectively, a therapeutically effective or a diagnostically effective amount of a humanized or composite anti-TAG-72 antibody or anti-TAG-72 antibody fragment according to claim
 1. 6. The composition of claim 5 wherein said antibody or fragment is, directly or indirectly, associated with or linked to an effector moiety having therapeutic activity, and said composition is suitable for the treatment of cancer.
 7. The composition of claim 6 wherein said effector moiety is a radionuclide, therapeutic enzyme, anti-cancer drug, cytokine, cytotoxin, or anti-proliferative agent.
 8. The composition of claim 7 wherein the radionuclide is selected from the group consisting of ⁴⁷Sc, ⁶⁷Cu, ⁶⁷Ga, ⁹⁰Y, ^(99m)Tc, ¹⁰⁵Rh, ¹¹¹In, ¹²³I, ¹²⁵I, ¹³¹I, ¹⁵³Sm, ¹⁷⁷Lu, ¹⁸⁶Re, ¹⁸⁸Re, ²¹¹At, ²¹²Bi, and chelates thereof.
 9. The composition of claim 5 wherein said antibody or fragment is, directly or indirectly, associated with or linked to a detectable label, and the composition is suitable for detection of cancer.
 10. The composition of claim 9 wherein the detectable label is a radionuclide or an enzyme.
 11. The composition of claim 10 wherein the radionuclide is selected from the group consisting of ⁶⁷Cu, ⁶⁷Ga, ^(99m)Tc, ¹⁰⁵Rh, ¹¹¹In, ¹²³I, ¹²⁵I, ³³¹I, ¹⁴⁵⁻¹⁵⁸Eu (all Europeum isotopes), ¹⁵³Sm, ¹⁴⁸⁻¹⁶¹Tb (all Terbium isotopes), ¹⁶⁶Ho, ¹⁷⁷Lu, ¹⁸⁶Re, ¹⁸⁸Re, and chelates thereof.
 12. A method for in vivo treatment of a mammal having a AG-72-expressing cancer which comprises administering to the mammal a therapeutically effective amount of a composition according to claim
 5. 13. A method of in vitro detection of TAG-72-expressing cancer cells which comprises contacting the cancer cells with a composition according claim
 5. 14. method of in vivo detection of TAG-72-expressing cancer cells in a mammal which comprises administering to the mammal a diagnostically effective amount of a composition according to claim
 5. 15. The method of claim 14 wherein said detection is in vivo tumor imaging.
 16. A method of treatment of cancer comprising (i) intravenously administering a radionuclide-labeled antibody composition of claim 5, (ii) thereafter, during surgery, detecting tumor cells using a radionuclide activity probe, and (iii) thereafter removing the detected tumor cells by excision.
 17. A method for in vivo diagnosis of cancer which comprises the steps of (a) administering to an mammal a diagnostically effective amount of the composition of claim 5, (b) allowing sufficient time for the imaging marker-antibody conjugates of said composition to become specifically localized upon at least one cancer cell, and (c) detecting the imaging marker in vivo at a site where said conjugate has become localized.
 18. A method of intraoperative therapy which comprises the steps of (a) performing upon a mammal having at least one cancer cell the method of claim 17 and (b) excising said cancer.
 19. The method of claim 18, wherein the imaging marker is ¹²⁵I or ¹³¹I.
 20. A kit comprising a composition according to claim 5 as an active ingredient together with instructions for use thereof to treat or detect cancer wherein the composition is reconstituted prior to said use. 